College Biology Lab Midterm Exam

What do you do during an analysis phase? Select all that apply. manipulate the hypothesis to fit the data graph the results judge whether the hypothesis is supported by the results make calculations based on the raw measurements

graph the results judge whether the hypothesis is supported by the results make calculations based on the raw measurements

Hydrogen peroxide is broken down to produce water and oxygen with the help of the enzyme catalase. Which of the following can be measured to determine the enzymatic activity? Select all that apply. how much hydrogen peroxide is used how much oxygen is produced how much catalase is used how much water is produced

how much hydrogen peroxide is used how much oxygen is produced how much water is produced

Laboratory safety protocols are established to protect (select all that apply) immunocompromised individuals in the biohazardous lab lab equipment and materials visitors to the lab that do not understand the experiment lab workers directly involved with the exercise

immunocompromised individuals in the biohazardous lab lab equipment and materials visitors to the lab that do not understand the experiment lab workers directly involved with the exercise

An enzyme reduces the energy necessary to start a chemical reaction. This means that the rate of the reaction will __. *increase *remain the same *decrease

increase

Which of the following does a lab coat protect against? Select all that apply. infectious microbes chemical fumes sharp insturments corrosive chemicals

infectious microbes corrosive chemicals

__ is used to detect the presence of starch.

iodine

The pH of an enzyme solution can alter the shape of the enzyme and its active site by affecting __ bonding.

ionic

In the enzyme experiment, what was the purpose of preparing a tube with water and hydrogen peroxide? it is a positive control to observe bubbling indicating the activity of hydrogen peroxide it is a negative control

it is a negative control

How does the pillbug react when confronted with a noxious substance? it does not move it moves away it moves toward the substance it rolls up

it moves away

When a pillbug is placed in a choice chapter, which of the following behaviors indicates a preference for a particular substance? it moves back and forth between the two chambers it moves away from the chamber with the test substance it rolls into a defensive position it spends more time in the chamber with the test substance

it spends more time in the chamber with the test substance

If you have a constant amount of substrate and add an increasing concentration of enzyme, what will happend to the rate of reaction? it will decrease it will increase it will stay the same

it will increase

Which of the following is the major difference between proteins, polypeptides and peptides? length site of synthesis type of monomer used type of bonds holding amino acids together

length

Which category of macromolecules is classified as a group based upon insolubility in water? carbohydrates lipids proteins nucleic acids

lipids

When measuring volume, which system of measurement is most accurate? metric system all should be exactly equal household system No answer text provided.

metric system

Which of the following approaches to the study of organisms enables direct viewing of the cells? biochemical genetic macroscopic microscopic

microscopic

Which distance is greater, a kilometer or a mile?

mile

Increasing the concentration of enzyme results in __ substrate interacting with the enzyme per unit volume. the same amount more less

more

Information about hazards posed by chemicals used in the lab can consistently be found in the lab manual on the Material Safety Data Sheet posted on the wall

on the Material Safety Data Sheet

When preparing your experiment, how many substances or conditions should you test at a time? as many as you like, as long as you control them all one two

one

Which of the following is the definition of the prefix "milli"? one tenth one thousandth one hundred one hundredth

one thousandth

Select all of the necessary items for making a wet mount. pipette clean slide cover slip timer bunsen burner

pipette clean slide cover slip

When should hands be washed? Select all that apply. prior to the lab exercise anytime hands are potentially contaminated right after you apply gloves and right before you remove them. after the lab exercise

prior to the lab exercise anytime hands are potentially contaminated after the lab exercise

Which topics belong in a scientific paper? Select all that apply. procedures introduction results core concepts

procedures introduction results

Which type of eukaryotic unicellular organisms are more animal like in that they lack a cell wall and obtain nutrients by engulfing food particles? fungi cyanobacteria protozoa algae

protozoa

What items should be disposed of in the sharps container? Select all that apply. razor blades broken glass pipettes used paper towels hazardous chemcials used gloves scalpel blades

razor blades broken glass pipettes scalpel blades

Which type of container would you dispose of a paper towel? biohazard container sharps container regular trash

regular trash

What do you do in each of the four phases report- experiment- analysis- hypothesis-

report- document your results and compose a report experiment- carry out the strategy and make measurements analysis- draw graphs; evaluate hypothesis hypothesis- create strategy; formulate hypothesis

Which of the following determines the quality and clarity of the microscope image? Select all that apply. resolving power magnification type of slide used contrast

resolving power magnification contrast

Of the following, what comes first in the hand washing process? A. rubbing hands with paper towel B. adding soap to the palm of the hand C. cleaning under nails with stick or brush D. running hands under water

running hands under water

Which of the following techniques can be used to view living specimens? Select all that apply. simple stain gram stain wet mount acid fast stain dry mount

simple stain wet mount

How do pillbugs detect the presence of food? sight smell hearing touch

smell

Suppose your hypothesis is "The braking distance grows linearly with speed". Which factor will you vary in order to test the hypothesis? weight of car road type brakes speed

speed

Suppose your hypothesis is "braking distance grows linearly with speed". What is the independent variable? speed car weight braking distance

speed

Plants store glucose as __.

starch

Scientific results that have undergone tests are (select all that apply) automated systematic reproducible certified

systematic reproducible

In order to be of scientific value, it is important that a hypothesis be ____. untrue factual correct testable

testable

In the enzyme experiment, how did you know the reaction was occurring? the consumption of the hydrogen peroxide decreased the contents of the test tube the production of water increased the volume in the test tube the production of oxygen caused bubbling in the test tube

the production of oxygen caused bubbling in the test tube

What is more important, to choose the correct hypothesis or to test the hypothesis well? neither are essential the right test is more important the right hypothesis is more important they are equally important

the right test is more important

In the enzyme experiment, what will happen if the test tubes are left an hour instead of 20 seconds? Select all that apply. the bubbles will continue to be produced until there is nor more solution left in the tube the tubes will all have the same height bubbles the bubbles will no longer be formed the bubbles will burst

the tubes will all have the same height bubbles the bubbles will no longer be formed the bubbles will burst

Which of the following are functions of proteins? Select all that apply. they can be for signaling they are a main storage molecule for carbon they act as catalysts they act to slow down chemical reactions they can be structural they are a main storage molecule for energy they can play a role in the immune system

they can be for signaling they act as catalysts they can be structural they can play a role in the immune system

At higher temperatures, molecules move more rapidly. True False

true

Not boiling the Benedict's test long enough can result in a false negative. True False

true

The concentration of an enzyme does not change. Therefore, enzymes can be reused and convert more substrate to product. True False

true

When measuring the length of irregular objects like bones, which of the following is important to do? use a straight edge or caliper to line up the ends of the object along a ruler use your eye to judge how the object lines up with the markings on a ruler measure along the length of the objet in short increments and then add them up

use a straight edge or caliper to line up the ends of the object along a ruler

In the chemical composition experiment, the negative control tube contained __.

water

The __ __ preparation allows one to observe the behavior and movement of microorganisms with a light microscope. (two words)

wet mount

When is protective eyewear crucial? when working with corrosive chemicals when using a bunsen burner when working with sharp objects when using the microscope

when working with corrosive chemicals

Why is it important to perform an experiment with a control? because in that way you assure the substances in the experiment are not harmful to lab personel without a control, a negative test could have been explained by some other factor causing a false positive without a control, a positive test could have been explained by some other factor causing a false positive

without a control, a negative test could have been explained by some other factor causing a false positive without a control, a positive test could have been explained by some other factor causing a false positive

When is it important to wear gloves in the lab? Select all that apply. working with sterile mammalian cells using the microscope dissections working with toxic chemicals

working with sterile mammalian cells dissections working with toxic chemicals

After viewing a slide under oil immersion, what could happen if you rotated the nosepiece the wrong direction before removing the slide? you could drag the 40x objective lens through the oil making it dirty you could mess up the focus, requiring further adjustment with the coarse focus knob you can break the slide

you could drag the 40x objective lens through the oil making it dirty

14 millimeters corresponds to __ centimeters.

1.4

Which of the following is the correct meaning of the prefix "milli"? 1/10000 1/10 1/1000 1/100

1/1000

Which lens could get dirty with oil if you move the revolving nosepiece in the wrong direction after viewing under oil immersion? 4x 100x 10x 40x

10x

In the brown paper bag test for fats, what is the minimun amount of drying time that must pass before determining the test results? 30 minutes 5 minutes 60 minutes 15 minutes

15 minutes

1 cup corresponds to approximately __ mL. 30 240 960

240

At what temperature on the Fahrenheit scale does water freeze? 4 degrees F 0 degrees F 25 degrees F 32 degrees F

32 degrees F

Normal body temperature when taken orally is about 98.6 degrees Fahrenheit. If you used a Celsius thermometer, what would the reading be? 25 degrees C 37 degrees C 100 degrees C 10 degrees C

37 degrees C

Room temperature water is often described as tepid or lukewarm. A Celsius thermometer placed in room temperature water should record approximately which temperature? 25 degrees C 0 degrees C 37 degrees C 100 degrees C

37 degrees C

58.6 centimeters corresponds to __ millimeters.

586

A wet mount is time consuming. True False

False

What are the advantages of using a wet mount? Select all that apply. the wet mount is safter to view pathogenic organisms The specimen can be viewed as living cells the contrast of the specimen is enhanced with the addition of water the motility of a specimen can be viewed under the microscope

The specimen can be viewed as living cells The motility of a specimen can be viewed under the microscope

During which of the following lab activities is it most important to tie back long hair? using a hotplate analysis of a urine sample Using a bunsen burner using the microscope

Using a bunsen burner

Which type of appendage is used by the pillbug to explore its environment? mouth parts antennae legs uropods

antennae

After they are used, reusable instruments or glassware that require sterilization should be placed in the sharps container autoclave biohazard bin sink

autoclave

Reusable dissection instruments or glass tube that requires sterilization should be placed in the sink autoclave trash biohazard bin

autoclave

A positive reaction for the iodine test for starch is indicated by a(n) __ color. green black red blue

black

Which of the follow shoes would be acceptable in the lab? Select all that apply. boots sneakers flip flops sandals

boots sneakers

Which of the following are phases in LearnSmart Labs' 4-phase model of the scientific method? Select all that apply experiment science evaluation hypothesis

experiment hypothesis

An enzyme at its optimum pH will produce a column of bubbles that is higher than when it is not at its optimum pH because it is less active. True False

false

To determine the activity of an enzyme, you can compare the concentration of the enzyme before and after the chemical reaction. True False

false

When the enzyme catalase is mixed with the substrate water, oxygen bubbles are produced. True False

false

Which of the following is a possible negative outcome in an experiment when contaminated glassware or spatulas are used? no effect false negatives false positives

false positives

Proteins are composes of building blocks called (two words) __ __.

amino acids

Which of the following unexpected results could occur if you used the catalase before it had a chance to reach your experimental temperature? Select all that apply. *in the experiment with the cooled enzyme, you would get no enzyme activity *in the experiment with the cooled enzyme, you would get a higher bubble column that expected *in the experiment with the over heated enzyme, you would get a higher bubble column than expected *in the experiment with the over heated enzyme, you would get no enzyme activity

*in the experiment with the cooled enzyme, you would get a higher bubble column that expected *in the experiment with the over heated enzyme, you would get a higher bubble column than expected

At very high temperatures enzymes can exhibit less activity or none at all. Which of the following could explain this? Select all that apply. * the active site has changed shape so that the substrate can not fit * the enzyme vaporizes and the solution now only contains substrate * the enzyme has denatured and cannot interact with the substrate

*the active site has changed shape so that the substrate can not fit *the enzyme has denatured and cannot interact with the substrate

What is the freezing point of water on the Celsius temperature scale? 0 degrees C 212 degrees C 100 degrees C 32 degrees C

0 degrees C

How many pounds are in 1 gram? 0.0022 lbs 2.2 lbs 1 lb 22 lbs

0.0022 lbs

Which is heavier, 1 gram or 1 pound?

1 pound

Which of the following statements is correct? A. food may be consumed in lab as long as the bench is wiped down and hands are washed B. Make up and contact lenses should not be applied in lab C. It is ok to modify lab procedures if you have carefully read it and have a way to do it more quickly D. It is fine to use your cell phone for calculations in lab as long as you are wearing gloves

Make up and contact lenses should not be applied in lab

When can food or beverages be brought into the lab? when the items are sealed Never food is not allowed but a water bottle is acceptable after the lab has been cleaned

Never

John places one drop of a sample solution onto a piece of brown paper. After a period of two minutes, he observes that the spot remains on the paper and concludes the sample contains oil. Has John drawn a valid conclusion? Why? Yes. All non-lipid substances will evaporate in 1 or 2 minutes. No. Water and other non-lipid substances may take up to 15 minutes to evaporate from the paper. No. Lipids will evaporate from the paper in 1 or 2 minutes. Only non-lipids will remain.

No. Water and other non-lipid substances may take up to 15 minutes to evaporate from the paper.

Which of the following are found in both plant and animal cells? chloroplasts cell walls Nuclei Centrioles

Nuclei

Match the steps of performing a cheek cell smear. Step 1 Step 2 Step 3 Step 4

Step 1- gently scrape inside of cheek Step 2- place scrapings on clean, dry slide Step 3- add one drop of stain Step 4- cover with coverslip

Put the steps of wet mount preparation in order. Step 1 Step 2 Step 3 Step 4 Step 5

Step 1- obtain a clean slide and cover slip Step 2- using a transfer pipette, obtain a drop of specimen and place it in the center of the slide Step 3- carefully place the cover slip over the drop of specimen Step 4- observe the preparation under the 10x objective lens Step 5- observe the preparation under 40x objective lens

Which of the following structures is present in animal cells but not plant cells? nuclei mitochondria centrioles cell walls

centrioles

In addition to storing carbon and energy, what else are lipids used for in cells? constructing membranes building cell walls in plants catalyzing reactions storing genetic information

constructing membranes

As you view a specimen on higher magnification, the amount of the slide seen in the field of view __, increases decreases remains the same

decreases

What is the role of a negative control in the test for protein? demonstrate the result of the test in the presence of proteins demonstrate the result of the test in absence of proteins make sure the reagent is active

demonstrate the result of the test in absence of proteins

The weight to volume ratio of a substance describes which physical property? mass size density temperature

density

The dispersal of fat molecules in water is called __. elixer emulsion compound solution

emulsion