Eryrhrocyte Inclusions

Normal erythrocytes are _____ µm in size. One-third of the central portion of the cell is pale, referred to as _____

7 to 8 central pallor

What are Pappenheimer bodies are caused by?

accelerated red cell division or impaired hemoglobin synthesis.

When the large reticulocytes normally found in the bone marrow are present in the peripheral blood, they are referred to as _____. These cells may be up to twice the size of normal mature red cells and are an indication of the bone marrow's response to severe anemia.

shift or stress reticulocytes

A mature erythrocyte containing siderotic granules is referred to as a _____, while an immature (nucleated) erythrocyte containing siderotic granules is known as a _____.

siderocyte sideroblast

iron-containing granules are called _____.

siderotic granules

Reticulocytes appear blue-gray on the Wright or Wright-Giemsa-stained smear and are referred to as polychromatophilic red cells. what stain is used?

supravital stain

to confirm the presence of Heinz bodies, hemoglobin H bodies, or reticulocytes, smears must be prepared after staining an aliquot of fresh whole blood with a _____

supravital stain

Cabbot rings

thin, red-violet-staining strands in the shape of rings, figure eights, or shapes of the letter B on Wright-stained smears

When do you see cabbot rings?

Cabot rings have been observed in megaloblastic anemia, lead poisoning, severe anemia, leukemia, myelodysplastic syndromes, and other cases of dyserythropoiesis

One of the functions of the spleen is _____, which refers to its ability to pluck out particles in red blood cells without destroying them.

pitting

what stain is used to detect siderosomes

prussian blue

When is coarse stippling seen?

Coarse basophilic stippling may be seen in lead poisoning (or other heavy metal poisoning), thalassemias, and anemias associated with abnormal heme synthesis such as sideroblastic anemia.

When are Howell Jolly bodies seen?

Howell-Jolly bodies may also be seen in hemolytic anemias, thalassemia, and sickle cell anemia.

Which inclusions may be observed on a Wright-stained peripheral blood smear if a patient has had a splenectomy?

Howell-Jolly bodies and Pappenheimer bodies

When a red cell precursor contains too much iron, the siderotic granules form a ring around the nucleus. If five or more siderotic granules form a ring around at least half the periphery of the nucleus of a nucleated red blood cell, the cell is referred to as a _____.

ringed sideroblast

What stain is used for Pappenheimer bodies?

Prussian blue stain

polychromatophilic RBCs

Reticulocytosis, immature RBCs

The presence of relatively evenly-distributed dark-blue particles or granules of varying size in the cytoplasm of erythrocytes is referred to as basophilic stippling which includes _____ and may indicate impaired hemoglobin synthesis, probably due to the instability of RNA in the young cell.

Ribosomes (RNA)

Howell-Jolly bodies

round, smooth, almost pyknotic, dark-purple bodies, contain DNA

The ringed sideroblast is associated with what?

The ringed sideroblast is associated with sideroblastic anemias and myelodysplasias.

How many Howell Jolly bodies do you normally see?

one

The basophilic stippling that is clinically significant is referred to as _____

coarse stippling

Pappenheimer bodies

degenerating cellular remnants that contain iron

Heinz bodies are composed of _____.

denatured hemoglobin

Heinz bodies are commonly associated with:

glucose-6-phosphate dehydrogenase (G6PD) deficiency and other red cell enzyme disorders

When are Pappenheimer bodies seen?

in certain types of anemia that are characterized by an increase in the storage of iron, such as sideroblastic anemia and thalassemia.

How do we detect shift or stress reticulocytes?

in the IRF channel