Exercise 2: Basic Light Microscopy

The ___ shapes the direction of the light before it encounters the objective

Condenser

What is the relationship between the depth of focus/ field of view and magnification?

Decreasing field of view and depth of focus with increased magnification

Why is Kohler Illumination used? How is this achieved?

Kohler Illumination results in an evenly illuminated field and a bright image without glare. This is achieved by adjusting the height of the condenser and closing the field diagram

An optical microscope produces an image when light is passed through glass or other optically transparent lenses. High quality ____ ____ can magnify an object over a thousand times

Light microscope

The measure of the number of image-forming light rays captured by the objective

Numerical Aperture (NA)

A special measuring ruler located on the ocular lens holder

Reticle

Dyes that bind tightly to cellular components

Stains

What is the Abbe Equation? You want the resolving power (d) to be as small or large as possible? How can this be done?

The Abbe equation tells us about the resolving power (d) of an objective. You want the (d) to be small as possible. This can be done by either shortening the wavelength or increasing the numerical aperture d=(.612 x wavelength)/(NA)

What is the purpose of using oil immersion?

The oil immersion captures more image forming light waves to improve the resolution and prevents refraction of light

What is the total magnification?

Total magnification is the product of the magnifying lenses through which light travels after leaving the object to produce the image (M total= M objective x M ocular)

What is the maximum resolving power of light microscopes?

Up to 100 nm

Which of the three lenses DO NOT magnify an image? a) condenser b) objective c) ocular d) all of the above magnify an image

a)

The ___ collects the light after it passes through the object produced a magnified real image

objective

The ___ produces a magnified virtual image of the real image

ocular

closest distance that two distinct parallel edges in the object can be separated and still be perceived as separate edges in the image produced by the lens

resolution

The amount of detail we can see

resolving power

Magnification (M) of an individual lens is defined as:

the length of an image produced by the lens/ length of the object which is magnified to produce the image