Experimental Design

What is the approximate percentage of dead cells?

30%

What is the approximate percentage of dead cells?

40%

What can you see?

All cells look the same

What do you expect to happen to the cells that are exposed to compound X?

Cells die

What would be the independent variable?

Compound conentration

What type of variable is the exposure time in this experiment?

Controlled cariable

The number of dead ells is one of the variables in our experiment. What type of variable is it?

Dependent variable

Why do we need to have experimental controls

Ensure that changes in the dependent variable depond on the independent variable

What is the most pressing scientific question to address, in order to tackle this public health problem?

Is the kidney toxicity caused by Ex-P?

What can you conclude from your initial findings?

It looks like Ex-P causes kidney injuries

There are four cell lines readily available in our lab. Which cell line would you use for the experiment?

Kidney epithelial cells

What can we conclude about the effect of Ex-P?

No conclusion can be made

Explore the cells in the microscope and interpret the results. What is your conclusion?

Our experimental setup can detect analgesic nephropathy

What would be your conclusion if most of the cells in the negative control were red?

Our experimental setup is flawed

What needs to be done to proof that Ex-P is responsible for the epidemic?

Reproduce results with replicates

What results are you expecting to see in the negative control?

The cells are not stained

What is your conclusion?

The negative control confirms the validity of the experiment

Why are the cells stained in pink?

The pink dye penetrates dead cells

Once you have performed the replicates, what are you going to do?

analyze data and draw conclusions

What is a hypothesis?

testable explanation