Final

100uL is equivalent to

0.1

What voltage is gel electrophoresis run at

100V

What volume of liquid would a p200 micropipette at this setting measure

155 ul

Match the phase

1st phase - light reaction 2nd phase- dark reactions

What is the optimal temp for most enzymes

20 to 40 degree Celsius

In order to separate PCR product bands produced by plant and GMO primers, a ____ agarose gel is required.

3%

How long is gel electrophoresis typically run for?

30 min

How much atp is generated by the complete oxidation of pyruvate into cow and h2o via aerobic respiration

36-38

What is the chemical equation for photosynthesis

6CO2 + 6H2O + light energy = C6H12O6 + 6O2.

What is the enzyme will will be using in lab

Alp

The linear relationship between the concentration solution and its light absorb acne is known as the

Beer-lambert law

The process by which organism chemically oxidize organic compounds as a mean of generating atp is

Cellular respiration

What is used to compare observed ratios for a given cross compare with predicted ratios

Chi-square analysis (χ2)

In prokaryotic cells catabolism of pyruvate takes place in the blank. Whereas in eukaryotes cells it occurs in the blank

Cytoplasm, mitochondria

What will happen in the experiment when light his the chloroplast

DPIP WILL BE REDUCED, dpip will change from blue to colorless, there will be an increase in light transmittance

What is the correct order for the steps of our amplification

Denaturation, annealing, extension

What is the test that can be used to detect proteins that are produced in gm crops

ELISA

Etc to

Electrons

A denatured enzyme can catalyze a chemical reaction

False

A molecular weight ruler aka ladder is used as a reference to determine the charge of your products

False

Enzyme activity typically occurs within a very broad ph range

False

If the results of a patient's two "random" glucose readings are 196 mg/dL and 200mg/dL, this person meets the criteria of diabetes mellitus.

False

Only glucose can be broken down to generate ATP.

False

Photosynthesis is a catabolic process

False

Plant cells are like red blood cells and can partially burst when placed in solutions of varying toxicity

False

simple diffusion requires energy input

False

During the process of gel electrophoresis combs are used to

Form the wells that samples will be placed in

What is the genetic composition of an organism called

Genotype

Name two types of peas that we will be working on

Germinated peas and dormant peas

CO2 is converted into a stable organic compound through a process known as

Glucose

Glycolysis to

Glucose

Who is known as the founder of genetics

Gregor Mendel

Can dominant alleles be masked by recessive alleles?

No

Toxicity is the measure of what

Osmotic pressure

What micropipette(s) could be used to measure out 175μL of liquid?

P200 and P1000

What does homozygous mean?

Same pair of alleles

during the dark reactions atmospheric c02 is brought into the leaf through tiny pores called

Stomata

What is added to gel that allows us to visualize dna

SyberSafe dye

What will happen when a red blood cell is placed in a hypertonic solution

The cell becomes shriveled

What is the P generation?

The first set of parents crossed

Fermentation generates energy

True

In the U.S., genetically modified foods do not have to labeled as such

True

Moving molecules against a concentration gradient requires energy

True

Potential allergic reactions to novel proteins and Antibiotic resistance from selectable markers are major arguments against/concerns associated with the GMO industry

True

What an enzyme reaction rate doubles for every 10 c increase in temp it's called the q10 effect

True

You must heat up the agarose to dissolve it.

True

aerobic respiration yields more atp then anaerobic respiration

True

What color will the product of todays experiment turn

Yelloq

What color-producing chemical will be used in today's lab to determine the concentration of glucose solutions through absorbance readings?

chromogen

What are autosomes?

chromosomes 1-22

What number do chromosomes of somatic cells contain?

diploid number (2n)

The act of placing genes for selected traits directly into crop plants is a form of genetic modification known as______________

genetic transformation

What is the end product of photosynthesis

glucose

At which stages of aerobic cellular respiration are ATP produced?

glycolysis, krebs cycle, chemiosmosis

Many species are unable to utilize oxygen as a final electron acceptor during cellular respiration due to:

lack of ETC proteins, lack of oxygen in the environment, lack of enzymes needed to inactivate free radicals

The movement of water molecules from a region of high free water concentration to a region of low free water concentration is known as

osmosis

What is the in vitro substrate for alkaline phosphatase (ALP) that we will be using in lab?

pNPP

DNA from Bacillus thuringiensis is incorporated into the genomes of crops to make them

pest resistant

ALP catalyzes the hydrolysis of _______________ attached to proteins or lipids.

phosphate groups

Large complexes of proteins and pigments (light-absorbing molecules) that are optimized to harvest light are called:

photosystems

What macromolecule is an enzyme?

protien

The difference between dialysis tubing and real cell membranes is that

real membranes are more selective

Blue-colored objects absorb which wavelength of light the most?

red

When leaf pigments absorb light, electrons are boosted to a higher energy level, providing energy to:

reduce NADP+ and produce ATP

Which of the following molecules can diffuse through a cell membrane

small molecules

What factors determine how fast water moves across a membrane

solute concentration difference & hydrostatic pressure

What is the name of the machine we will use in todays lab to measure the amount of light that is absorbed by a solution

spectrophotometer

Primers serve as _________ for amplifying the target region of the DNA to be copied.

start and stop points

What type of buffer is used to run gel electrophoresis?

tae

What happens if either the enzyme or substrate is limited

the enzyme's reaction rate will remain constant

DNA polymerase is derived from a bacterium that lives in

High-temp steam vents

There is a blank relationship between the color of a solution and the wavelength of light it best absorbs

Inverse

What will happen when a red blood cell is placed in distilled water

Lysis will occur

Match the photosystem to the specialized pigment molecules.

PSI: P700 PSII: P680

What is the appearance of a particular trait

Phenotype

Karen's cycle to

Pyruvic acid