Final review bio lab

100μL is equivalent to:

0.1mL

Ocular lenses found in most compound light microscopes a magnification of..?

10x

What is the optimal temperature for most enzymes?

20 to 40 degrees Celsius

You are using a compound microscope and you have the objective lens set to 40x. What is the total magnification you are using to view the specimen?

400x

What is the enzyme did we use in lab?

ALP (Alkaline phosphatase)

If you get stuck while researching papers/topics or citing a paper what is the best solution?

Ask an NSU librarian

Your heart rate, or pulse, is the number of times your heart ______

Beats per minute

The linear relationship between the concentration of a colored solution and its light absorbance is known as the ______.

Beer-Lambert Law

What color-producing chemical was used in lab to determine the concentration of glucose solutions through absorbance readings?

Chromogen

Which has a smaller wavelength: electrons or visible light?

Electrons

If the results of a patient's two "random" glucose readings are 196 mg/dL and 200mg/dL, this person meets the criteria of diabetes mellitus. true or false

FALSE

TRUE OR FALSE: Enzyme activity typically occurs within a very broad pH range.

FALSE

TRUE OR FALSE: Plant cells are like red blood cells and can potentially burst when placed in solutions of varying tonicity.

FALSE

TRUE OR FALSE: Simple diffusion requires energy input.

FALSE

To view a specimen under an electron microscope, does it have to be living.

False

The variable that is manipulated or varied by the researcher in an experiment is the:

Independent Variable

There is a(n) __________ relationship between the color of a solution and the wavelength of light it best absorbs.

Inverse

Your resting heart rate is___________

Is when your heart is pumping the least amount of blood When your heart rate is at its slowest

If you were to attend lab in person, what is the proper attire you must wear before entering the lab? (Select all that apply)

Long Pants Closed-Toe Shoes Lab-Coat

What will happen when a red blood cell is placed in distilled water?

Lysis will occur

In an experiment designed to determine the rate of reaction based on the content of each beaker, the hypothesis that states there will be no difference in the outcome of the experiment is:

Null Hypothesis

The movement of water molecules from a region of high free water concentration to a region of low free water concentration is known as ____________________.

Osmosis

Tonicity is the measure of what?

Osmotic presssure

What micropipette(s) could be used to measure out 175μL of liquid?

P200 P1000

ALP catalyzes the hydrolysis of _______________ attached to proteins or lipids.

Phosphate groups

A scanning electron microscope (SEM) does what?

Produce 3-D images of the surfaces of objects.

What macromolecule is an enzyme classified as?

Protein

Two groups being able to reproduce the results of an experiment conducted in the exact same way follows the principles of:

Similarity of Causation

what molecules can diffuse through a cell membrane?

Small molecules

What factors determine how fast water moves across a membrane?

Solute concentration difference Hydrostatic pressure

What is the name of the machine we used in lab to measure the amount of light that is absorbed by a solution?

Spectrophotometer

TRUE OR FALSE: Moving molecules against their concentration gradient requires energy.

TRUE

TRUE OR FALSE: When an enzyme's reaction rate doubles for every 10 degree Celsius increase in temperature it's called the Q10 effect.

TRUE

What will happen when a red blood cell is placed in a hypertonic solution?

The cell becomes shriveled

Independent variables are usually plotted on the X-axis of a graph, whereas dependent variables are usually plotted on the Y-axis

True

The cause behind the results of an experiment will stay the same if we repeat the experiment tomorrow follows the principles of:

Uniformity

What is precision?

a measure of how close a series of measurements are to one another

If you were given a sample of bacteria to analyze, which microscope would be best for quickly identifying the specimen?

compound

Parts of a microscope

eyepiece, objective lens, fine adjustment know, course adjustment knob, stage, stage clips, diaphragm

Do dissecting microscopes allow higher magnification than compound microscopes?

false

TRUE OR FALSE: A denatured enzyme can catalyze a chemical reaction.

false

What is accuracy?

how close a measurement is to the true value

What determines resolution?

magnification, visual acuity, lens diameter, lens quality

Steps of the Scientific Method

observation, hypothesis, experiment, analyze, repeat

What is the in vitro substrate for alkaline phosphatase (ALP) that we used in lab?

pNPP

The difference between dialysis tubing and real cell membranes is that

real membranes are more selective

Blue-colored objects absorb which wavelength of light the most?

red

What happens if either the enzyme or substrate is limited?

the enzyme's reaction rate will remain constant

What color did the product of the experiment (pNP) turn in the enzyme lab?

yellow