Gel Electrophoresis

targeting a gene with a mutation

Gel electrophoresis can be used for

visualization

Glycerol is added to bromophenol blue dye to make the DNA solution denser, so DNA can sink into the wells of the gel.

negative

The cathode charge the wells and DNA samples are placed at. (starting point)

positive

The cathode the DNA migrates towards. The smaller DNA fragments move faster and farther than larger DNA segments. "Run to Red"

buffer solution

The gel floats in a __________ within a chamber between two electrodes. Ensures that the electric current goes through the whole tank and maintains the movement of the ions in the solution.

wells

The spaces that are loaded with DNA samples. using a Micropipette.

agarose gel

a jelly-like slab used to track movement of DNA based on their size and weight.

Gel electrophoresis

a technique used to separate segments of DNA based on their size using an electric current applied to a porous gel.

ethidium bromide or methylene blue

the chemical used to stain the bands, allowing them to fluoresce. DNA is stained using Sybr Green dye to track them.

DNA paternity testing

the process of using electrophoresis to compare DNA in a paternity case.

DNA fingerprinting forensics

the process of using electrophoresis to find a murder suspect using DNA.