Lab #1

If the diameter of your microscope field is 1 mm at low power (10×) and your ocular lens is 10×, then what is the diameter of your field when you switch to the 50× objective lens?

0.2 mm

If your ocular lens is 10×, and you are using your 45× objective lens to view a slide, the total magnification is ________.

450x

This is the formula for calculating the diameter of an unknown microscope field.

Diameter of field A × Total Magnification of field A = Diameter of field B × Total Magnification of field B

Should you always being observations with the oil immersion lens?

No

Parfocal

The slide should be almost in focus when changing to higher magnifications.

Histopathology is the use of microscopes to view tissues to diagnose and track the progression of diseases. Why are thin slices of tissue ideal for this procedure?

Thin slices are used so that pathologists can clearly see individual cells for their assessment. Too many layers of cells makes it difficult to focus and hard to see what changes have occurred in the tissues.

How do you clean the lenses of your microscope?

With a special lens paper and cleaner.

The microscope field is the ______________

area you see through the lenses of the microscope.

You are looking at very lightly stained cells under the microscope, and you want to increase the contrast so that you can see them "better." You ________ the iris diaphragm to ________ the light.

close, dim

The __________ is a substage lens that concentrates light on the specimen.

condenser

As total magnification increases, the depth of field ________

decreases.

As you increase the total magnification of the specimen, the size of the field ________

decreases.

As you increase the total magnification of the specimen, the working distance ________

decreases.

When using the higher power objective lenses, you would use this part of the microscope to focus the specimen.

fine adjustment knob

When observing a slide under a microscope, begin focusing with the __________-power objective lens in place.

lowest

You begin your observations using the ________ lens and the ________ adjustment knob.

lowest-power, coarse

The working distance for a 10x objective lens is ________

much greater than the working distance for the 100x objective lens.

The total magnification of an object can be found by __________

multiplying the power of the objective lens by the power of the ocular lens.

The _______ of the microscope carries three or four objective lenses.

nosepiece

You have learned that the objective lens magnifies the object on the slide, producing the ________ image, and that the ocular lens magnifies that image, forming the ________ image.

real, virtual

The ability to discriminate two close objects as separate is called __________

resolution (or resolving power).

Before putting away the microscope in the storage cabinet you must observe all of the following except ________

rotate the highest power objective lens in position.

The microscope slide rests on the __________ while being viewed.

stage

When you want to study a slide under a microscope, you place it on the ____________.

stage.

To transport a microscope, hold it __________

upright, with one hand on its arm and the other hand supporting its base.

You are observing the letter "e" under a compound microscope. Which of the following indicates how the image appears as you view it through the ocular lens?

ə