Lab #1 Terminology, Microscopy, and Cells: Exercise Two-Microscopy

True

Grasp the plug (not the cord) and unplug the microscope; holding the plug, wind the cord loosely around your hand, loop the plug through the cord once, and place the cord on the stage of the microscope. True or False?

Parfocal

Having corresponding focal points all in the same plane: having sets of objectives or eyepieces so mounted that they may be interchanged without varying the focus of the instrument (as a microscope) with which they are used.

Objective Lens (4, 10, 40, 100) X Eyepiece Lens (10)

How do you calculate the Overall Magnification?

Field-of-view

The diameter of the field of an optical light microscope is the field number, which is the diameter of the field view in mm measured at the intermediate image plane. The field number is typically listed on the microscope eyepiece.

How much bigger a sample appears to be under the microscope than it is in real life

What is magnification?

Compound Light Microscope: an optical instrument for forming magnified images of small objects, consisting of an objective lens with a very short focal length and an eyepiece with a longer focal length, both lenses mounted in the same tube. Origin of compound microscope.

What type of microscope is this?

Working distance

Working Distance and Parfocal Length. Microscope objectives are generally designed with a short free working distance, which is defined as the distance from the front lens element of the objective to the closest surface of the coverslip when the specimen is in sharp focus.

True

You should carry the microscope with one hand under the base and the other hand grasping the frame; place the microscope in the storage cubicle with the corresponding letter. True or False?

False

You should leave the slide specimen on the stage of the microscope when you have finished. You should not store the slide back in its proper slide box. True or False

False

You should use the fine focus knob only with the low power objective (4x)

False

You should wrap the electrical cord around the frame of the microscope when you store, not the stage of the microscope. True or False?

True

You will need to set up and focus several specimens on the midterm and final practical exam. True or False?

Dissecting microscope or Stereo or Stereoscopic microscope

is an optical microscope variant designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it.

Total magnification

is determined by multiplying the eyepiece power (usually 10x) by the objective lens in place. For example, a 10x eyepiece and a 4x objective yields a total magnification of 40x (40 power)

Depth of focus

refers to the depth of the specimen layer which is in sharp focus at the same time, even if the distance between the objective lens and the specimen plane is changed when observing and shooting the specimen plane by microscope.