Micro bio exam 1 (Q&A)

Which of the following are not considered microorganisms? a) Mosquitoes b) Fungi c) Protozoa d) Viruses e) Bacteria

a) Mosquitoes

Which of the following are present in viruses? a) None of the choices are correct. b) Mitochondria c) Flagella d) Endoplasmic reticulum Pigments

a) None of the choices are correct.

Please select all of the following which are found in all cells regardless of cell type. a) cell membrane b) cell wall c) cytoplasm d) DNA e) Organelles

a) cell membrane c) cytoplasm d) DNA

Please select all of the following which are contents of the cell cytoplasm. a) chromosome b) ribosome c) water d) glycocalyx e) fimbriae

a) chromosome b) ribosome c) water

Of the photosynthetic bacteria, cyanobacteria produce __________ as a product of photosynthesis, but the green and purple sulfur bacteria do not produce this substance. a) oxygen b) chlorophyll c) ATP d) carbon dioxide

a) oxygen

Which of the following is the correct way to type the scientific name of this bacterium? a) Fusobacterium Nucleatum b) f.nucleatum c) Fusobacterium nucleatum d) Fusobacterium

c) Fusobacterium nucleatum

The Gram stain, acid-fast stain, and endospore stain a) are used on a wet mount of the specimen. b) use heat to force the dye into cell structures. c) use a negative stain technique. d) are differential stains. e) have outcomes based on cell wall differences.

d) are differential stains.

In the current taxonomic system, the level that includes the largest number of organisms would be Kingdom? (true or false)

FALSE (This system first divides all organisms into 3 domains: Archaea, bacteria, and eukarya)

How to detect noncluturable microbe?

-viable but nonculturable microbes can be detected by their DNA

These are unique to eukaryotes? A) ER, Golgi, Cilia B) ER, Golgi, cell wall C) Nucleoid, Cytosol, RNA D) Chloroplast, DNA, plasmids

A) ER, Golgi, Cilia

I am a bacteria and I have a single flagellum on each of two opposite ends. I am: -monotrichous -lophotrichous -amphitrichous -peritrichous

Amphitrichous

Which biochemical activities is used in MacConkey's agar? a) H2S formation b) Lactose fermentation c) Sulfur reduction

b) Lactose fermentation

You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? a) Colorless b) Purple c) Red/Pink

b) Purple

-The type of microscope in which you would see brightly illuminated specimens against a black background is a) phase-contrast. b) dark-field. c) electron. d) fluorescence. e) bright-field.

b) dark-field

When humans manipulate the genes of microorganisms, the process is called a) bioremediation. a) bioremediation. b) genetic engineering. c) epidemiology. d) taxonomy. e) immunology.

b) genetic engineering.

Numerous cases of microcephaly have been observed in the newborn babies of women who were infected with Zika virus during pregnancy. This led to the proposed hypothesis that Zika virus was causing the birth defects. This is an example of a) theory development. b) inductive reasoning. c) hypothesis testing. d) deductive reasoning.

b) inductive reasoning.

In lab, Isaac was given a mixed culture. His objective is to isolate single colonies. What should he use to accomplish this goal? a) Hockey stick, spread plate technique, and 4 degree Celsius incubation temperature b) Sterile swab, loop dilution, TSB c) Inoculating loop, incinerator, streak plate method, 37 degree Celsius incubator d) Loop dilution, TSA, hockey stick, 37 degree Celsius incubator

c) Inoculating loop, incinerator, streak plate method, 37 degree Celsius incubator

You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram negative bacteria, like Escherichia coli, be? a) Red/Pink b) Colorless c) Purple

c) Purple Notes: If there is a reversal of crystal violet and safranin stains, then the cross-link between iodine and crystal violet won't happen, and thus during decolorization the safranin will fade away, leaving all of the cells colorless. When the crystal violet stain is used last, it will dye all of the cells purple regardless of being gram-positive or negative and this will be the end result.

Which scientist showed that anthrax was caused by the bacterium Bacillus anthracis? a) Ignaz Semmelweis b) Louis Pasteur c) Robert Koch d) Antonie van Leeuwenhoek e) Joseph Lister

c) Robert Koch

A microbiologist inoculates Staphylococcus aureus into a culture medium. Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture. What is the most likely explanation? a) The culture medium must be selective. b) The culture medium must be differential. c) The culture is contaminated. d) The microbiologist used too much inoculum. e) The incubation temperature was incorrect.

c) The culture is contaminated.

The primary purpose of staining cells on a microscope slide is to a) kill them. b) secure them to the slide. c) add contrast in order to see them better. d) see motility. e) enlarge the cells.

c) add contrast in order to see them better.

If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying power of the objective lens if the ocular lens is 10X? a) 950X b) 850X c) 100X d) 85X e) 95X

e) 95X