MicroBiology Lab Final questions

Antibiotics work against which types of microbes? Answers: a. bacteria b. viruses c. fungi d. all of the above

A

If a person has only the A-antigen on their red blood cells, what blood type will they have? Answers: a. Type A- b. Type A+ c. Type B+ d. Type O-

A

If the negative control wells change color then what probably happened? a. there was cross contamination from a positive well or micropipette b. the reagents are defective c. the 1st antibody is expired d. the second antibody is expired

A

If you spread the plates with the cell spreader in the order 10-6, 10-7 and 10-8, then you would: Answers: a. need to flame it with burning alcohol between each plate b. only need to flame it one time with burning alcohol

A

In order to get exactly 1 ml with an Eppendorf style pipette you push the plunger down to the: Answers: a. first stopping position b. second stopping position

A

In which type of person will the first antibody be present that will stick to the sides of the well? a HIV+ b. HIV-

A

Pipetting 0.1 ml from a dilution tube to an agar plate ___________ change the dilution factor. Answers: a. will b. will not

A

Potable water means that the water is: Answers: a. safe enough to drink b. safe enough to swim in but not safe to drink

A

What color will the negative control wells be after performing the test? a. colorless b. brown

A

What happens to Brilliant green pH indicator when acid is produced? Answers: a. it yellows b. it turns purple c. it turns pink

A

What is the MPN standard for drinking water? Answers: a. zero b. 20 c. 40 d. 1100

A

What should you do if the negative controls turn color? a. repeat the experiment and be more careful b. diagnose the patient as HIV+ c. report the patient as being HIV-

A

Which ELISA strategy detects the presence of the HIV virus in the patient's serum? a. direct ELISA b. indirect ELISA

A

Which group of organisms should Penicillin work best against? Answers: a. Gram positives b. Gram negatives

A

Which of the following blood types is a dominant trait? Answers: a. Type A b. Type O

A

Which set of tubes has dsBGLB broth in them? Answers: a. 10 ml tubes b. 1 ml tubes c. 0.1 ml tubes d. all of the above

A

How many antigen binding sites does each antibody have? Answers: a. only one b. two for each Y-shaped molecule

B

If a person is has a genotype of IBi, what blood type will they have? Answers: a. Type A b. Type B c. Type O

B

If a plate hypothetically has 750 colonies on it you would: Answers: a. count it and record it as 750 b. not count it and record it as TNTC c. not count it and record it as TFTC

B

If the disinfectant killed the bacteria then the TSB tube should be. Answers: a. positive b. negative

B

If you had 250 colonies on a plate with a 10-4 dilution factor then how would you record the final answer for the concentration in the original culture tube? Answers: a. 0.25 X 10^4 b. 2.5 X 10^6 c. 25 X 10^6 d. 250 X 10^5

B

If you perform a blood typing test and there is clumping in the B well but not in the A well or the Rh well then the person is. Answers: a. A+ b. B- c. AB- d. O+

B

Perform a Punnett square to see what percentage of the offspring will be type O if one parent has a genotype of IAi and the other parent is IBi. Answers: a. 0% b. 25% c. 50% d. 75% e. 100%

B

Pipetting 1 ml into a 9 ml dilution blank results in a ___________ fold dilution. Answers: a. 1 b. 10 c. 100 d. 1000

B

To have type O blood you must be: Answers: a. homozygous dominant b. homozygous recessive c. heterozygous

B

What are disinfectants used on? Answers: a. living tissue b. inanimate objects only

B

What color will the positive control wells be after performing test? Answers: a. colorless b. brown

B

What difficult sugar is being fermented during this test? Answers: a. glucose b. lactose c. mannitol d. arabinose

B

What does the acronym MPN mean? Answers: a. many practical numerals b. most probable number c. mostly potable numerically

B

What range of colonies constitutes a countable plate? Answers: a. 0-30 b. 30-300 c. 100-1000 d. 0-10,000

B

Which ELISA strategy is usually the first step in diagnosing a patient as HIV positive? a. direct ELISA b. indirect ELISA

B

Which antibody will have the enzyme linked to it? a. 1st antibody from patient serum b. 2nd antibody (anti-human)

B

Each sample is run how many times? a. it only needs to be run once b. duplicate c. triplicate

C

If a person has type B+ blood, which of the following blood types would they not be able to receive in a blood transfusion? Answers: a. B- b. B+ c. AB+ d. O-

C

If all nine test tubes turn positive then what would the combination of positives be? Answers: a. 0-0-0 b. 1-1-1 c. 3-3-3 d. 9-9-9

C

If an ELISA test is negative then what happened to the enzyme? a. it was denatured by the incubation temperature b. it was on the wrong antibody and did not stick to the side of the well c. there was not patient antibody present so there was nothing for the linked antibody to stick to

C

If you pipette 0.1 ml from a dilution tube with a dilution factor of 10-3 onto an agar plate then what will the dilution factor on the agar plate be? Answers: a. 10-2 b. 10-3 c. 10-4 d. 10-5

C

Pipetting 1 ml into a 99 ml dilution blank results in a ___________ fold dilution. Answers: a. 1 b. 10 c. 100 d. 1000

C

The clumping reaction seen during a blood typing test is called: Answers: a. a hematocrit b. a cross reaction c. agglutination

C

The presence of which antigen determines that the blood is positive? Answers: a. A-antigen b. B-antigen c. Rh-antigen

C

What does UV light damage in living cells? Answers: a. cell walls b. cell membranes c. DNA d. mitochondria

C

What group of bacteria is the MPN test targeting? Answers: a. Gram positive rods b. Gram positive cocci c. Coliforms d. Mycobacteria

C

What is the solid contaminated surface we are using in this lab? Answers: a. test tubes b. Petri dishes c. glass beads

C

What is the target of the cell if an antibiotic inhibits protein synthesis? Answers: a. cell wall b. cell membrane c. ribosomes d. PABA

C

What must be present in order to consider the tube to be a positive reaction? Answers: a. it must turn pink b. it must turn yellow c. it must have a gas bubble

C

If you get a combination of positives of 3-1-0 then what would the MPN index be? Answers: a. 7 b. 11 c. 23 d. 43

D

The size of zones of inhibitions are expressed as what? Answers: a. area in cm2 b. circumference in cm c. radius in inches d. diameter in mm

D

What drawback or limitation does UV light have as a microbial control method? Answers: a. it does not kill but only slows growth b. it is very expensive c. it is highly toxic and requires evacuation strategies for safety d. it does not penetrate well so is limited to surfaces

D

What is the exposure time for the disinfectants? Answers: a. 30 seconds b. 1 minute c. 3 minutes d. 10 minutes

D

If a tube has a dilution factor of 10-6 then how many times more dilute is it than the original sample? Answers: a. 2 times b. 10 times c. 100 times d. 1000 times e. one million times

E