Microbiology Mycoplasma and Ureaplasma

Colonization of M. hominis and U. urealyticum is directly related to?

# of sexual partners and immune status.

Mycoplasma and Ureaplasma can be frozen at what temperature?

-70

How long does it take for M. pneumoniae to grow in culture?

21 days

M. fermentens strain incognitus is referred to as?

AIDS related mycoplasma

Special transport media (PPLO agar) is needed for Mycoplasma and Ureaplasma specimens which includes what protective additives?

Albumin and sucrose

M. pnuemoniae causes what clinical infection that is associated with a dry cough that persists for weeks?

Atypical pnuemonia (walking)

Mycoplasma is resistant to what type of antibiotics due to not having a call wall?

Beta lactams

Lab diagnosis of Mycolplama and Ureaplasma is difficult because they are all susceptible to drying & heat and do not stain due to not having what?

Cell wall

Mycoplasma adhere to epithelial cells of mucosal surfaces in the respiratory & genital tracts and is NOT eliminated by?

Cilial action or urination

Serology is the tradition method of diagnosis for M. pneumoniae. What serology test is used?

Cold agglutinin antibody titer

What antibiotics are used to treat Mycoplasma and Ureaplasma infections?

Erythromycin, tetracycline, or cipro (reported emergence of high level sensitivity)

What is needed for Mycoplasma to grow in culture which requires it to be grown on complex enriched media?

Fatty acids & cholesterol

When looking at a culture of Mycoplasma through a dissecting microscope, what distinct morphology will you see?

Fried egg appearance

Mycoplasma is susceptible to what environmental factors?

Heat & drying

What organism is considered an emerging respiratory pathogen that may be involved in the pathology of T cells, and results in infection that disseminates in HIV patients?

M. fermentens strain incognitus

What organism infects the lower and upper genital tracts of women and causes PID, postpartum fevers and sepsis, pyelonephritis, and neonatal infections?

M. hominis

What genus of Mycoplasma is never NF so it is always significant when isolated and is only associated with respiratory infections?

M. pnuemoniae

List the 3 human pathogens associated with Mycoplasma and Ureaplasma.

M. pnuemoniae M. hominis U. urealyticum (controversial)

What bacteria was first isolated in the late 1800's from cows?

Mycoplasma

_________ are the smallest, free living organisms in nature and are common contaminants of cell cultures.

Mycoplasma and Ureaplasma

Mycoplasma and Ureaplasma are emerging __________ pathogens for certain patient populations.

Opportunistic

M. pnuemoniae causes a large percentage of community-acquired pneumonia leading to epidemics in what types of settings?

Schools, prisons, and military barracks

A combination of clinical and serologic findings are the best way to diagnose M. pnuemoniae but when PCR is not available what is the best method to use?

Serology

M. hominis and U. urealyticum are both opportunistic pathogens that are transmitted how?

Sexually (especially women)

What organism infects the upper genital tracts and in women causes reproductive disorders and in men causes non-gonococcal urethritis in about 10% of infected men, and can also cause neonatal infections in utero?

U. urealyticum

Mycoplasma and Ureaplasma should NOT be collected on what kind of swabs?

Wooden or cotton