MLT 111 microscope
What is the maximum resolution achievable with the light microscope? 2 microns 2 millimeters 0.2 mm 0.2 microns 0.2 nm
0.2 microns
Which of the following parameters is most critical for image quality? Specimen slide size Field diaphragm setting Inter-ocular distance Ocular magnification Coverslip thickness and quality
Coverslip thickness and quality
Which of the following contributes the most information about high spatial frequency features in the specimen? Diffraction Refraction Absorption Reflection Dispersion
Diffraction
An objective lens with a high NA guarantees good resolution. True False
False
What is the ultimate criterion by which you should evaluate your selection of optics and accuracy of alignment? Image quality The back focal plane diffraction pattern Condenser NA Objective NA The resolution equation
Image quality
Which of the following describe the two sets of conjugate planes that exist in the microscope? Illuminating and diffracting Imaging and illuminating Imaging and diffracting Diffracting and refracting Real and virtual
Imaging and illuminating
Which of the following is the best method for aligning the microscope and illuminating the specimens? Abbé illumination Aperture illumiation Aperture matching Quartz-halogen illumination Köhler illumination
Köhler illumination
In what three ways does a microscope increase the information you obtain from a specimen? Choose an anwer in the list below. Illumination, magnification, and imaging. Contrast, illumination, and magnification. Definition, resolution, and contrast. Magnification, resolution, and contrast. Discrimination, definition, and detection.
Magnification, resolution, and contrast.
The most highly corrected objective is which of the following? Achromat (25/0.45, 160/0.17) Plan apochromat (40/1.0 oil, 160/0.17, Plan Apo) Plan achromat (100/1.25, 160/0.17, Plan) Apochromat (40/0.75, 160/0.17, Apo) Plan achromat, NA = 1.3 (100/1.3 oil, 160/0.17, Plan)
Plan apochromat (40/1.0 oil, 160/0.17, Plan Apo)
Which of the following are directly affected by adjusting Köhler illumination? Magnification Resolution Contrast Resolution and contrast All of the above
Resolution and contrast
Which of the following components is involved in the most steps when aligning Köhler illumination? The condenser The stage The objective The oculars The field diaphragm
The condenser
As the refractive index of a material increases, what happens to the speed of light? The speed of light decreases The speed of light is constant The speed of light increases The speed of light varies as n2 The speed of light varies as √n
The speed of light decreases
Which of the following is the correct equation for calculating the resolution limit of the light microscope?
resolution = λ/ NA (objective + NA (Condenser)
