Module 7 quiz

Which of the following might complicate the interpretation of ChIP-Seq data?

Because histones can be moved around or ejected from the chromatin, DNA that is only sometimes wrapped around a histone might not be sequenced.

Which of the following is the most likely location of an insulator sequence?

Between two genes

How are eukaryotic transcription factors regulated? (Check all that apply.)

Covalent modification Binding of an effector molecule Protein-protein interaction

Into which gene of the CRISPR-Cas system are fragments of bacteriophage DNA inserted?

Crispr

Select the four most common types of molecules to which ncRNAs bind.

DNA RNA Proteins Small Molecules

CpG islands are associated with which of the following?

DNA methylation

A repressor protein would enhance the ability of TFIID to bind to the TATA box of the promoter.

False

Activator proteins bind to silencer sequences and repressor proteins bind to enhancer sequences.

False

Prior to hormone binding, receptors for steroid hormones are usually found in the nucleus of the cell.

False

Which of the following is a region in DNA that binds to a steroid receptor?

GRE

The ncRNA HOTAIR recruits what type of proteins to target genes?

Histone-modifying complexes

The CRISPR-Cas system in bacteria is most similar to what system in vertebrates?

Immune System

Which of the following is a major finding from the ENCODE project?

More than 80% of the human genome sequence is linked to a biological function.

Abnormal levels of miRNAs are associated with nearly all forms of what type of human disease?

Neurological disorders

In order of their occurrence what are the steps in RNA interference?

Processing of RNA transcript by Drosha and DGCR8, transport to cytoplasm, processing by Dicer, binding to proteins to form a RISC, binding to target mRNA

piRNA sequences are complementary to __________.

RNA from a transposable element

You are working in a lab that studies snoRNAs. You mutate the antisense sequences in a C/D box snoRNA to completely different sequences. What results do you expect compared to wild type snoRNA? Check all that apply.

Reduced binding of the snoRNA to the rRNA Reduced methylated ribose in the rRNA

Select functions that ncRNAs can perform. Choose all that apply.

Scaffold Guide Ribozyme Blocker Decoy Alteration of Protein function or stability

What general transcription factor is most often affected by regulatory transcription factors?

TFIID

A particular cell contains all of the standard histones but lacks several histone variants. Which of the following MAY be true of this cell?

The cell will express different sets of genes than other cells in the same organism.

What might happen if the insulator between two genes is missing?

Transcription factors influencing one gene's expression may abnormally influence a neighboring gene's expression.

If a portion of a transcription factor's domain is structurally similar in a variety of organisms, it is called a motif.

True

Nucleosome location may be changed by a process called ATP-dependent chromatin remodeling.

True

A mutation in the iron response protein (IRP) that made it incapable of binding iron would result in __________.

consituitve transferrin receptor and no ability to stimulate ferritin expression

Which ncRNAs work to inhibit the epithelial-mesenchymal transition in adult cells, thus acting as tumor suppressors?

miR-200 family