Package 16 Photosynthesis

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What was the purpose of the experiment in which Calvin and his colleagues injected algae with 14C-labeled CO2, incubated the cells for various time periods, and then separated the radiolabeled molecules with paper chromatography?

To determine the order of reactions in the Calvin cycle

In C4 plants, the first product of carbon fixation is:

a four-carbon molecule

What chemical reaction is catalyzed by the enzyme rubisco?

carbon fixation

True or false: In the reduction phase of the Calvin cycle, ATP is the source of electrons used to reduce a C-O bond to a C-H bond.

f

True or false: The term "C3" was derived from the 3-carbon sugar that represents the end product of photosynthesis.

f

The end product of phase 2 of the Calvin cycle is G3P. This molecule is used to synthesize ---- and to regenerate the starting material ----bisphosphate.

glucose RU

Photorespiration is favored when plants are exposed to a(n) --- and --- environment.

hot dry

In a cool climate, a C3 plant uses ______ a C4 plant to fix CO2.

less energy than

PEP carboxylase can attach CO2 to phosphoenolpyruvate, resulting in the synthesis of a four-carbon molecule called

oxaloacetate

In the Calvin cycle, CO2 is:

reduced

The Calvin cycle is affected by certain environmental conditions, including light intensity, ----availability, and ----

water temperature

What advantages do C4 plants have over C3 plants in warm, dry climates?

-They can keep their stomata partially closed during the day to conserve water. -They can minimize photorespiration.

The G3P produced at the end of the second phase of the Calvin cycle is directly used for which of the following?

-regenerate ribulose bisphosphate -make glucose or other carbohydrates

What are the three phases of the Calvin cycle?

1.Carbon Fixation 2.Reduction and carbohydrate production 3.Regeneration of ribulose bisphosphate

In the Calvin cycle, for every SIX carbon dioxide molecules that are incorporated into a carbohydrate such as glucose, ----molecules of ATP are hydrolyzed and ----molecules of NADPH are oxidized.

18 12

The Calvin cycle can be very energy-expensive. How many molecules of ATP and NADPH respectively are required to incorporate one carbon dioxide molecule into the six-carbon sugar being formed?

3 ATP, 2 NADPH

The Calvin cycle uses energy from ---, and high-energy ----supplied by NADPH

ATP Electrons

Which of the following are products of the light reactions that are used by the Calvin cycle to make carbohydrates?

ATP NADPH

Which of the following summarizes the events of the first phase of the Calvin cycle?

Atmospheric carbon dioxide is fixed into a five carbon sugar.

Why are C3 plants so-named?

Because 3PG is a three-carbon molecule

What molecule is reduced in the Calvin cycle?

CO2

The end product of phase 2 of the Calvin cycle is G3P. This molecule is used to synthesize ----and to regenerate the starting material---- bisphosphate.

Carbohydrates RuBP

In plants, which of the following is polymerized to form starch?

Glucose

Which of the following is the primary output of the Calvin cycle?

Glyceraldehyde-3-phosphate

What is carbon fixation?

Incorporation of carbon into an organic molecule that is not a gas

Which of the following happens to the molecule formed by the addition of carbon dioxide to ribulose bisphosphate?

It is immediately split into two more stable three carbon molecules.

What is the source of electrons used to reduce the C-O bond of 3-phosphoglycerate during the Calvin cycle?

NADPH

What two molecules can be attached to RuBP by the enzyme rubisco?

O2 CO2

Which of the following differentiates PEP carboxylase from rubisco?

PEP carboxylase does not bind oxygen

What chemical reaction is catalyzed by the enzyme PEP carboxylase?

Phosphoenolpyruvate + CO2 → oxaloacetate

In the last phase of the Calvin cycle, what molecule is regenerated so that the next cycle can occur?

Ribulose bisphosphate

What two chemical reactions are catalyzed by rubisco?

RuBP + O2 → 3PG + phosphoglycolate RuBP + CO2 → 2 3PG

What happens during photorespiration?

Rubisco attaches O2 to RuBP, and CO2 is produced.

Which of the following is the purpose of phase 3 of the Calvin cycle?

To create a new 5-carbon sugar for the "next" Calvin cycle


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