Pre-Lab Quiz 5 (Properties of Enzymes)

What color will the product of today's experiment (pNP) turn?

Yellow

What is the enzyme we will be using in lab today?

ALP

What is the in vitro substrate for alkaline phosphatase (ALP) that we will be using in lab?

pNPP

T/F When an enzyme's reaction rate doubles for every 10 degree Celsius increase in temperature it's called the Q10 effect.

True

What happens if either the enzyme or substrate is limited?

the enzyme's reaction rate will remain constant

What macromolecule is an enzyme classified as?

protein

What is the optimal temperature for most enzymes?

20-40 degrees Celsius

T/F A denatured enzyme can catalyze a chemical reaction.

False

T/F Enzyme activity typically occurs within a very broad pH range.

False

ALP catalyzes the hydrolysis of _______________ attached to proteins or lipids.

Phosphate groups