Quiz 4 - Microscope

100x = 1.8 mm = ___________ um

1,800

Oil Immersion

100x = 1,000x

Low

10x = 100x

High

40x = 400x

Scanning lense

4x = 40x

Field of vision

All that is seen under a particular magnification. Decreases as magnification increases

Depth of field

All the total possible planes of focus

Course focus knob

Allows for a large degree of focusing; used only with scanning objective

Fine focus knob

Allows for a small degree of focusing for fine detail

Iris

Controls how much light you allow to go into your eye

When you increase magnification, you ___________ field of vision and light intensity

Decrease

How to: length of each sample

Length of field over # of samples

Stage Orrafice

Light that goes through sample

Stage manipulators

Move sample up, down, left and right

Total magnification

Multiplying the power of the ocular lens by power of the objective lens

Resolution

The ability to distinguish two separate points as being separate and distinct

Objective lense

The lens closest to the specimen. (small in front)

Ocular lens

The lens you look through. 10x

Why is oil used for the oil immersion lense?

The oil refracts light less than air directing more light into the objective lens for a clearer image.

What happens to the field of vision as the magnification increases? Decreases?

There is an inverse relationship between the field of vision and the magnification, which means, as magnification increases, the field of vision decreases vise versa.

The diameter of the field of vision

This was done by actually measuring the diameter of the field of vision using a stage micrometer

Mechanical stage

Where the slide is placed and can be moved

Magnification

an enlarged image resulting from the product of the ocular and objective lense

Parfocal

set of objective lens so mounted that they may be interchanged without varying the focus of microscope