Bio Ch. 3 Lab Questions

Select the specimen that could be viewed using a light microscope.

Bacteria Cells Tissues

Select all of the following that can be studied with bright-field microscopy.

Bacteria Tissues Cells

Staining cells such that their color is different from the background enhances which quality of the cells?

Contrast

Which image quality factor is defined as the ability to make an object appear larger?

Magnification

Which of these challenges are microscopes used to overcome?

Many biological specimens are too small to see with the unaided eye.

Select the benefits of wet mount sample preparation for microscopy.

Motility of specimens can be observed. Living organisms can be viewed. The preparation is relatively simple and fast.

Which of these will further magnify the image produced by the objective lens?

Ocular lenses

Which part of the bright-field microscope is a lens that is located closest to the eye during use?

The ocular lenses

Resolution is an image quality factor that involves being able to see fine _____, which is essential for image clarity.

detail

Changing the magnification of an image with a microscope changes the portion of the slide that is visible, or the

field of view

Bright-field microscopes use a combination of lenses and _____ to view a specimen.

light

If you are unable to see something with your eyes, you can increase the _____ to make it look bigger.

magnification

The lenses of a bright-field microscope are responsible for the _____ of the object you are viewing.

magnification

The total magnification of an image is determined by ______ the magnification power of the ocular lens and the magnification power of the objective lens used.

multiplying

The goal of microscopy is to create a magnified image of

objects that are too small to be seen with the unaided eye.

The portion of a slide that is visible through the eyepiece is called the field of

view

If a specimen is viewed using a 100× objective and the ocular lenses, what is the total magnification?

1000x

Oil immersion microscopy uses which objective lens?

100x

Which objective lens is used with a sample that has been prepared with a wet mount

10× low-power lens

Select the components used by a bright-field microscope to view a specimen.

A light source Glass lenses

Which lens has a magnification of 40×?

High-power objective

A benefit of using the oil immersion lens is a reduction in scatter, which

Increases resolution

Which lens has a magnification of 10×?

Low-power objective

Which image quality factor is defined as the ability to distinguish fine detail?

Resolution

What is enhanced when a stain is used to make a sample, or parts of a sample, darker than the background in a slide?

contrast