Bio-Lab final

what is 100 uL equivalent close to?

0.1mL

Ocular lenses found in most compound light microscopes a magnification of..?

10X

What volume of liquid would a P200 micropipette at this setting (pictured below) measure out? 115

155Ul

Optimum temperature for most enzymes

20-40 degrees Celsius

How much ATP is generated by the complete oxidization of pyruvate into CO2 and H2O via aerobic respiration?

36-38

You are using a compound microscope and you have the objective lens set to 40x. What is the total magnification you are using to view the specimen?

400x

What is the chemical equation for photosynthesis?

6CO2 + 6H2O ------> C6H12O6 + 6O2

What is the enzyme that will be used in todays lab?

ALP

The linear relationship between the concentration of a colored solution and its light absorbance is known as the ______.

Beer-Lambert Law

CO2 is converted into a stable organic compound through a process known as

Carbon fixation

The process by which organisms chemically oxidize organic compounds as a means of generating ATP is?

Cellular Respiration

What is used to compare observed ratios for a given cross compare with predicted ratios?

Chi-square analysis (X2)

What color-producing chemical will be used in today's lab to determine the concentration of glucose solutions through absorbance readings?

Chromagen

What are autosomes?

Chromsomes 1-22

If you were given a sample of bacteria to analyze, which microscope would be best for quickly identifying the specimen?

Compound

In prokaryotic cells, catabolism of pyruvate takes place in the __________________. Whereas, in eukaryotic cells, it occurs in the _________________________.

Cytoplasm, mitochondria

What will happen in the experiment when light hits the chloroplasts?

DPIP will be reduced, DPIP will change from blue to colorless, there will be an increase in light transmittance

What number do chromosomes of somatic cells contain?

Diploid number (2n)

Which has a smaller wavelength: electrons or visible light?

Electrons

The first offspring produced from a monohybrid cross is called the...

F1 generation

Dissecting microscopes allow higher magnification than compound microscopes. T/F?

False

Enzyme activity typically occurs within a very broad pH range. T/F?

False

If the results of a patient's two "random" glucose readings are 196 mg/dL and 200mg/dL, this person meets the criteria of diabetes mellitus. T/F?

False

Only glucose can be broken down to generate ATP?

False

Plant cells are like red blood cells and can potentially burst when placed in solutions of varying tonicity.

False

Name the two types of peas that we will be working with in the cellular respiration lab.

Format, germinating

Genetic composition of an organism is the?

Genotype

What is the end product of photosynthesis (for the plant)?

Gluclose

What is the substrate for Glycolysis, Krebs, ETC

Glucose, pyruvate, electrons

At which stages of aerobic cellular respiration are ATP produced?

Glycolysis, Krebs cycle, Chemiosmosis

What factors determine how fast water moves across a membrane? Select all that apply.

Hydrostatic pressure, Solute concentration difference

The variable that is manipulated or varied by the researcher in an experiment is the:

Independent variable

There is a(n) __________ relationship between the color of a solution and the wavelength of light it best absorbs.

Inverse

Your resting heart rate is___________

Is when your heart is pumping the least amount of blood When your heart rate is at its slowest

1st and 2nd phase of photosynthesis

Light Reactions, Dark reactions

What will happen when a red blood cell is placed in distilled water?

Lysis

What are the general steps in order of the scientific method?

Making observations, developing hypothesis's, designing experiments, analyzing results, repeat

Is photosynthesis a catabolic process?

No

Tonicity is the measurement of what?

Osmotic pressure

What micropipette(s) could be used to measure out 175μL of liquid?

P200 and P1000

PSI and PSII photosystem

P700 and P680

Appearance of a particular trait is the?

Phenotype

ALP catalyzes the hydrolysis of _______________ attached to proteins or lipids.

Phosphate groups

Large complexes of proteins and pigments (light-absorbing molecules) that are optimized to harvest light are called:

Photosystems

A scanning electron microscope (SEM) does what of the following?

Produce 3-D images of the surfaces of objects

The difference between dialysis tubing and real cell membranes is that

Real membranes are more selective

Blue-colored objects absorb which wavelength of light the most?

Red

When leaf pigments absorb light, electrons are boosted to a higher energy level, providing energy to:

Reduce NADP+ and produce ATP

Two groups being able to reproduce the results of an experiment conducted in the exact same way follows the principles of:

Similarity of Causation

Which of the following molecules can diffuse through a cell membrane? Mark all that apply.

Small molecules

What is the name of the machine we will use in today's lab to measure the amount of light that is absorbed by a solution?

Spectrophotometer

During the dark reactions, atmospheric carbon dioxide (CO2) is brought into the leaf through tiny pores called: ___________

Stomata

What will happen when a red blood cell is placed in a hypertonic solution?

The cell becomes shriveled

What happens if either the enzyme or substrate is limited?

The enzyme reaction rate will remain constant

What is the P generation?

The first set of parents crossed

Aerobic respiration yields more ATP than anaerobic respiration.

True

Fermentation generates energy?

True

When an enzyme's reaction rate doubles for every 10 degree Celsius increase in temperature it's called the Q10 effect. T/F?

True

The cause behind the results of an experiment will stay the same if we repeat the experiment tomorrow follows the principles of:

Uniformity

What determines resolution?

Visual Acuity, Lens Quality, Lens Diameter, Magnification

What does homozygous mean?

When the same pair of alleles compress a trait

What color will the product of todays experiment turn?

Yellow

Accuracy vs. Precision

accuracy: how close experimental value is to accepted value; precision: how closely measured values agree with each other

Objective lens on microscope

enlarges the image of the specimen

Many species are unable to utilize oxygen as a final electron acceptor during cellular respiration due to:

lack of ETC proteins, lack of oxygen in the environment, lack of enzymes needed to inactivate free radicals

No difference in outcome is the ____ hypothesis

null

What is the in vitro substrate for alkaline phosphatase (ALP) that we will be using in lab?

pNPP

Coarse Focus Adjustment Knob

this part moves the stage up and down to help you get the specimen into view