Body Fluids, Ch10, Semen Analysis

A semen sample for semen analysis should generally be received at the testing site within what period of time? Please select the single best answer A)One hour B)Two hours C)Three hours D)Four hours

A

What is the optimal time to begin initial microscopic examination of a semen specimen, assuming liquefaction is complete? Please select the single best answer A)30 minutes B)2 hours C)4 hours D)8 hours

A

Which of the following are directly involved in the production of semen? More than one answer is correct. Please select all correct answers A)Prostate B)Pituitary gland C)Seminal vesicles D)Bulbourethral gland E)Urethra

A, C, D

A semen sample is received in the laboratory for evaluation of fertility. No sperm are observed upon initial microscopic examination of the sample. How should you proceed? Please select the single best answer A)Report as "no sperm observed" and cancel the remainder of the analysis. B)Mix the original specimen, centrifuge an aliquot, and evaluate again. C)Mix the original specimen and make two slides; air-dry and stain to examine for presence of sperm.

B

What is the lower reference limit for semen volume according to the World Health Organization, WHO Laboratory Manual for the Examination and Processing of Human Semen, 5th edition? Please select the single best answer A)0.5 mL B)1.5 mL C)3.5 mL D)4.5 mL

B

Which of the following represents the lower reference limit for semen volume? Please select the single best answer A)0.5 mL B)1.5 mL C)3.0 mL D)6.0 mL

B

Which of the following statements is true regarding normal viscosity of semen? Please select the single best answer A)When a sample of semen is allowed to fall freely from a disposable pipette or glass rod, the semen will fall in a water-like stream, if viscosity is normal. B)When a sample of semen is allowed to fall freely from a disposable pipette or glass rod, the semen will fall in small drops with very little trailing thread if viscosity is normal. C)When a sample of semen is allowed to fall freely from a disposable pipette or glass rod, the semen will form a thread more than 2 cm long, if viscosity is normal.

B

Which of the following statements regarding semen analysis are TRUE? More than one answer is correct. Please select all correct answers A)Evaluation of a single semen sample will provide enough information to determine male fertility status. B)Examination of two or three samples is recommended to better evaluate male fertility status. C)Semen analysis can be used to evaluate the success of vasectomy and vasectomy reversal.

B, C

Which of the following statements are TRUE for semen specimen collection and transport? More than one answer is correct. Please select all correct answers A)Specimen should be transported to the testing site within three hours. B)Specimen should be maintained between 20 - 37oC after collection prior to delivery to the laboratory. C)Specimen should be collected after seven days of sexual abstinence. D)Onsite collection is optimal for obtaining the most accurate results.

B, D

What is the recommended MINIMUM number of spermatozoa that should be counted on each side of the hemocytometer when a manual sperm count is performed using an improved Neubauer hemocytometer? Please select the single best answer A)50 B)100 C)200 D)400

C

Which of the following describes normal semen pH ? Please select the single best answer A)Strongly acidic B)Slightly acidic C)Slightly alkaline D)Strongly alkaline

C

"Round cells" in a semen sample refer collectively to which of the following types of cells? More than one answer is correct. Please select all correct answers A)Epithelial cells B)Red blood cells (RBCs) C)White blood cells (WBCs) D)Spermatids

C, D

What is the MINIMUM number of sperm that should be counted to achieve the greatest accuracy when performing a sperm count using an improved Neubauer hemocytometer? Please select the single best answer A)20 on each side of the hemocytometer for a total of 40. B)50 on each side of the hemocytometer for a total of 100. C)75 on each side of the hemocytometer for a total of 150. D)200 on each side of the hemocytometer for a total of 400.

D

A sperm concentration of 25 x 106 spermatozoa/ mL would be considered an abnormally low concentration, according to the WHO 5th edition. Select true or false

false

Condoms are a good way to collect semen specimens for fertility analyses. Select true or false

false

Liquefaction of a semen specimen always occurs immediately after ejaculation. Select true or false

false

Semen viscosity can be estimated by aspirating the sample into a wide-bore plastic disposable pipette, allowing the semen to drop by gravity and observing the length of the thread that is formed. A semen sample that has normal viscosity will produce a thread more than 2 cm long. Select true or false

false