BSC 118 Chapter 20 MyLabsPlus

This is a DNA fingerprint exhibiting samples from a victim, two suspects, and the crime scene. Which of these DNA fragments is common to both the victim and Suspect 2?

C

the sticky end of the DNA restriction fragment shown here will pair with a DNA restriction fragment with the sticky end _____

-ACGT

This is a DNA fingerprint exhibiting samples from a victim, two suspects, and the crime scene. Which of these DNA fragments is common to both the victim and Suspect 1?

B

This is an animation of gel electrophoresis. Which of these DNA molecules is the shortest?

E

which of the following is the correct order for one cycle of polymerase chain reaction (PCR)?

denature DNA; anneal primers; extend primers

the reason for using Taq polymerase for PCR is that _______

it is heat stable and can withstand the heating step of PCR

gel electrophoresis separates DNA fragments on the basis of what characteristic

length

in gel electrophoresis DNA molecules migrate from _______ to _______ ends of the gel

negative ... positive

the unpaired nucleotides produced by the action of restriction enzymes are referred to as _________

sticky ends

Why is Suspect 1 considered more likely to have committed the crime than Suspect 2?

the crime scene sample contains DNA fragments from both the victim and suspect 1

what is the enzymatic function of restriction enzymes?

to cleave nucleic acids at specific sites