Ch 14 Vaccines. Microbiology

Of the following components, which ones are required for the amplification of a specific section of DNA using the polymerase chain reaction?

- DNA Primers - DNA polymerase that is heat stable

Which plasmid characteristics would be required for optimal expression of a DNA fragment to yield a peptide?

-a region with multiple sites for restriction digestion with different enzymes - a DNA sequence that signals for trafficking of the plasmid to the nucleus - a strong promoter for expression of the inserted DNA fragment

Sort the following restriction enzymes into what type of end cut they will result in. Choose either "Sticky Ends" or "Blunt Ends" based on information from this table.

Sticky Ends: -BamHI -EcoRI -HinDIIII Blunt Ends: -EcoRII -MspI -Hpal

In a laboratory setting, which of the following scenarios would benefit from utilizing a technique such as PCR?

You would like to make copies of a bacterial gene of interest for sequencing and characterization. You have a fastidious organism that is hard to grow in large numbers and you need to isolate an operon for transferring to a bacterium for expression. You want to study the effects of a protein on different cell types, but the bacterial species that the protein originates from is considered a pathogen.

In an effort to yield a high percentage of transformed bacteria, you may choose to utilize electroporation to increase the probability of what occurring?

that nearly all of the cells present in the field will take up at least one copy of the plasmid