CH4: Prokaryotic & Eukaryotic Cells
Prokaryotic
"Naked" DNA Prokaryotic, Eukaryotic or both
Many pathogenic (disease-producing) bacteria have _______ that protect them from phagocytosis by host cells.
capsules
Compared to other bacteria, mycoplasmas are somewhat atypical because they do not have a _______.
cell wall
Which of the following is not a structure of prokaryotic cells? A. chromosome B. chloroplast C. capsule D. ribosome E. flagella
chloroplast
Sequence the path of a solute from the external environment to the cytoplasm of a prokaryotic cell.
external environment --> capsule --> cell wall --> plasma membrane --> cytoplasm
Which of the following processes uses membrane proteins that act as channels or carriers allowing ions or large molecules to move across the plasma membrane without using energy? A. active transport B. simple diffusion C. facilitated diffusion D. osmosis
facilitated diffusion
Which of the following bacterial structures is necessary for chemotaxis? A. flagella B. plasmids C. metachromatic granules D. capsules
flagella
Prokaryotic and Eukaryotic
Cell membrance Prokaryotic, Eukaryotic or both
The cell walls of bacteria and eukaryotes are very similar.
False
The motility of bacteria with flagella occurs through a series of "runs" and _____.
tumbles
Three basic bacterial shapes?
1. coccus 2. bacillus 3. spiral
Prokaryotic and Eukaryotic
Cells Prokaryotic, Eukaryotic or both
Acid-fast bacteria demonstrate unique staining properties because of a special protein layer found in their cell walls.
False
Which of the following is a likely outcome, based on the unique cell structure of members of the genus Mycoplasma? A. Mycoplasma species are susceptible to disruption by lysozymes. B. Mycoplasma species can release endotoxins when they lyse. C. Mycoplasma species have very flexible cells that can pass through bacteriologic filters. D. Mycoplasma species are susceptible to penicillin. E. Mycoplasma species usually stain gram-positive.
Mycoplasma species have very flexible cells that can pass through bacteriologic filters.
Which statement best describes what happens when a gram-positive bacterium is placed in an aqueous solution of lysozyme and 10% sucrose? A. No change will result; the solution is isotonic B. Water will move into the cell C. Water will move out of the cell D. The cell will undergo osmotic lysis E. Sucrose will move into the cell from an area of higher concentration to one of lower concentration
No change will result; the solution is isotonic
Eukaryotic
Nucleus Prokaryotic, Eukaryotic or both
Prokaryotic
Only bacteria cells Prokaryotic or Eukaryotic
Which of the following is a difference between gram-positive and gram-negative cells? A. Only gram-negative cells have a lipopolysaccharide (LPS) layer. B. Only gram-positive cells have peptidoglycan that is made of a carbohydrate backbone. C. Gram-negative cell walls contain teichoic acids. D. Gram-positive cells contain less peptidoglycan than do gram-negative cells.
Only gram-negative cells have a lipopolysaccharide (LPS) layer.
Eukaryotic
Plants, animals, fungi, protists Prokaryotic or Eukaryotic
Which of the following eukaryotic cells do NOT contain a cell wall? A. animals B. plants C. algae D. fungi
animals
Which of the following is NOT characteristic of the glycocalyx found in bacteria? A. creates a slimy, slippery coating that prevents bacteria form attaching to surfaces B. if firmly attached, contributes to bacterial virulence C. a structure that can be visualized by negative staining D. a viscous coating surrounding the cell made of polysaccharide, polypeptide, or both
creates a slimy, slippery coating that prevents bacteria form attaching to surfaces
The structural framework in a cell is the A. cytoskeleton. B. endoplasmic reticulum (ER). C. extracellular matrix. D. endomembrane system. E. plasma membrane.
cytoskeleton.
Which of the following membrane transport mechanisms takes place in eukaryotic cells but NOT in prokaryotic cells? A. endocytosis B. active transport C. facilitated diffusion D. osmosis
endocytosis
When essential nutrients are depleted, some strains of bacteria form ______, dormant structures that form within cells.
endospores
A Gram stain of a wound reveals purple, spherical-shaped bacteria that divide and remain attached in grapelike clusters. These are referred to as __________. A. gram-positive staphylococci B. gram-positive tetrads C. gram-positive streptococci D. gram-negative spirochetes
gram-positive staphylococci
You are observing a Gram stain of rectangular-shaped microorganisms that are linked in a chain and stain purple. How would you describe these bacteria using the correct terminology for the cell shape and arrangement? A. gram-positive coccobacilli B. gram-positive streptobacilli C. gram-positive tetrads D. gram-negative staphylococci
gram-positive streptobacilli
Within the cytoplasm of bacterial cells are reserve deposits where certain nutrients accumulate. These are called __________. A. vacuoles B. inclusions C. ribosomes D. lysosomes
inclusions
The plasma membrane is considered a barrier to the environment because ________. A. only water and small molecules may penetrate the membrane B. the membrane is the outermost structure of the cell C. it controls the passage of molecules into the cell D. it is a rigid structure E. no molecules may pass through it
it controls the passage of molecules into the cell
The antibiotic polymyxin B combines with phospholipids. This will cause ________. A. leakage of cell contents B. sporulation C. stoppage of protein synthesis D. binary fission E. loss of motility
leakage of cell contents
All of the following are found in the cell walls of gram-positive bacteria except ________. A. lipid A B. teichoic acid C. peptidoglycan D. N-acetylglucosamine E. lipoteichoic acid
lipid A
Which of the following organelles breaks down worn-out organelles? A. rough endoplasmic reticulum (ER) B. lysosomes C. smooth endoplasmic reticulum (ER) D. mitochondria E. Golgi apparatus
lysosomes
What carries instructions for making proteins from the nucleus into the cytoplasm? A. ATP B. Rough ER C. ribosomes D. mRNA E. DNA
mRNA
Where in a cell is ATP made? A. nucleus B. chloroplasts C. mitochondria D. lysosomes E. ribosomes
mitochondria
With a light microscope, which of the following would allow discrimination between eukaryotes and prokaryotes? A. observation of the plasma membrane B. observation of photosynthetic pigment C. observation of nuclei D. observation of the ribosomes E. observation of flagella
observation of nuclei
The term describing bacteria with flagella distributed over the entire surface of the cell is _____ .
peritrichous
A eukaryotic cell can ingest a prokaryotic cell by ________. A. digestive vacuolization B. active transport C. pinocytosis D. phagocytosis E. osmosis
phagocytosis
Which of the following pairs is mismatched? A. glycocalyx - adherence B. pili - reproduction C. cell wall - toxin D. cell wall - protection E. plasma membrane - transport
pili - reproduction
Which of the following is not a functionally analogous pair? A. circular bacterial chromosome; -linear eukaryotic chromosomes B. pili; centrioles C. peptidoglycan; cellulose D. bacterial flagella; 9 + 2 flagella E. 70S ribosomes; 80S ribosomes
pili; centrioles
What structure acts as a selective barrier, regulating the traffic of materials into and out of the cell? A. cytoskeleton B. nuclear envelope C. extracellular matrix D. endomembrane system E. plasma membrane
plasma membrane
In a phospholipid bilayer, water interacts with the ______ of the phospholipid molecules.
polar heads, hydrophilic heads
You have isolated a motile, gram-positive cell with no visible nucleus. You can assume this cell has A. ribosomes B. mitochondria C. an endoplasmic reticulum D. a Golgi complex E. all of the above
ribosomes
Indicate the bacterial structures that are likely to be antigens, to which host antibodies bind, marking the invader for phagocytosis (more than one). A. fimbriae B. ribosomes C. cell wall D. apsule E. nucleoid F. flagella G. plasmids
A. fimbriae C. cell wall D. apsule F. flagella
Identify antibacterial strategies that would likely be selectively toxic for bacteria (more than one). A. inhibition of peptidoglycan synthesis B. inhibition of microtubule function C. inhibition of fimbriae synthesis D. interfering with lysosomal function E. interfering with translation at 70s ribosomes
A. inhibition of peptidoglycan synthesis C. inhibition of fimbriae synthesis E. interfering with translation at 70s ribosomes
Which of the following processes occurs in bacterial plasma membranes, but not in eukaryotic plasma membranes? A. ATP synthesis B. protein synthesis C. facilitated diffusion D. RNA synthesis E. active transport
ATP synthesis
Which of the following statements about ribosomes is FALSE? A. Cells with a high rate of protein synthesis have large numbers of ribosomes. B. All eukaryotic and prokaryotic cells contain ribosomes. C. Antibiotics that interfere with protein synthesis harm both prokaryotic and eukaryotic ribosomes. D. Eukaryotic ribosomes are larger (80S) than prokaryotic ribosomes (70S).
Antibiotics that interfere with protein synthesis harm both prokaryotic and eukaryotic ribosomes.
Which of the following is true of bacteria that have atypical cell walls, such as those in the genus Mycoplasma? A. These atypical bacteria usually stain gram-negative. B. Although small, these atypical bacteria cannot pass through most filters. C. Bacteria with atypical cell walls are generally among the smallest bacteria that can reproduce outside a host cell. D. These atypical bacteria have the same plasma membrane typical of bacteria.
Bacteria with atypical cell walls are generally among the smallest bacteria that can reproduce outside a host cell.
Prokaryotic and Eukaryotic
DNA Prokaryotic, Eukaryotic or both
Eukaryotic
DNA is in a nucleus Prokaryotic, Eukaryotic or both
Which of the following statements is true? A. One bacterial cell produces many endospores. B. Endospores allow a cell to attach to solid surfaces and to surfaces within the host. C. Endospores are extremely durable structures that can survive high temperatures. D. Endospores are reproductive structures.
Endospores are extremely durable structures that can survive high temperatures.
There are some structures that are common to all prokaryotic cells, and others that are only found in some bacterial species or strains. Select the structures below that are found in all bacterial cells. (more than one) A. cell wall B. flagella C. capsule D. pilus (pili) E. fimbriae F. ribosomes G. plasma membrane H. cytoplasm I. nucleoid
F. ribosomes G. plasma membrane H. cytoplasm I. nucleoid
Bacteria do not have chloroplasts and therefore cannot perform photosynthesis.
False
Epulopiscium fishelsoni is 0.5 mm in diameter. It contains no nucleus and has peptidoglycan. Due to the size of this cell, it should be classified as a eukaryote.
False
Penicillin is more effective against gram-negative bacteria than gram-positive bacteria because it specifically interferes with the synthesis of lipopolysaccharide.
False
Which of the following is part of the endomembrane system? A. flagellum B. cytoskeleton C. ribosomes D. Golgi apparatus E. mitochondria
Golgi apparatus
Which one of the following pairs is correctly matched? A. Golgi complex--membrane formation and secretion of proteins B. lysosomes--secretion C. centrioles--cellular respiration D. mitochondrion--cell division
Golgi complex--membrane formation and secretion of proteins
Which of the following statements is true regarding the principles of osmosis? A. The relative concentrations of solutes inside and outside the cell have no influence on the direction of osmosis. B. If a cell is a hypotonic solution, water leaves the cell in an attempt to balance to concentration of solutes and reach equilibrium. C. If a cell is a hypertonic solution, water leaves the cell in an attempt to balance to concentration of solutes and reach equilibrium. D. If a cell is in an isotonic solution, no water moves across the membrane because equilibrium has been reached.
If a cell is a hypertonic solution, water leaves the cell in an attempt to balance to concentration of solutes and reach equilibrium.
Which of the following statements best describes what happens to a cell exposed to polymyxins that destroyed phospholipids? A. In an isotonic solution, nothing will happen B. In a hypotonic solution, the cell will lyse C. Water will move into the cell D. Intracellular contents will leak from the cell E. Any of the above might happen
Intracellular contents will leak from the cell
All of the following are true of the gram-negative outer membrane except: A. It has polysaccharide antigens that are useful in bacterial identification. B. It contains lipopolysaccharide. C. It contains lipids also known as endotoxin. D. It contains enzymes for energy synthesis. E. It is a part of the gram-negative cell wall
It contains enzymes for energy synthesis.
Which of the following statements about a gram-negative cell wall is FALSE? A. It contains teichoic acid. B. It maintains the shape of the cell. C. It functions as an endotoxin. D. It includes a thin layer of peptidoglycan.
It contains teichoic acid.
A strain of Streptococcus pneumoniae is no longer able to synthesize its capsular polysaccharide. What is a likely outcome? A. It will no longer be motile B. It will be readily phagocytized upon entering the host C. It will produce more potent toxins which damage the host D. It will no longer be able to carry out fermentation E. It will not be able to appropriately transport ions across its plasma membrane
It will be readily phagocytized upon entering the host
A strain of Neisseria gonorrhoeae has been genetically altered and can no longer produce fimbriae. What is a likely outcome? A. It will no longer be able to secrete exotoxins. B. It will be unable to adhere to host tissue and establish infection C. It will be unable to carry out aerobic respiration D. It will be readily phagocytized upon entering the host E. It will demonstrate increased resistance to antibiotics
It will be unable to adhere to host tissue and establish infection
Eukaryotic
Larger Prokaryotic or Eukaryotic
Prokaryotic and Eukaryotic
Microscopic Prokaryotic, Eukaryotic or both
Why is penicillin selectively toxic to bacterial cells but harmless to human cells? A. The glycocalyx of an animal cell provides a barrier through which penicillin cannot pass. B. Penicillin is rapidly metabolized and destroyed by human cells. C. Human cells actively transport penicillin out of the cell. D. Penicillin is inactivated within lysosomes of human cells. E. Penicillin specifically weakens peptidoglycan, which is found only in bacterial cells.
Penicillin specifically weakens peptidoglycan, which is found only in bacterial cells.
Which of the following statements is false? A. Plant cells have a cell wall, while animal cells do not have a cell wall. B. Plant cells do not have mitochondria. C. Both plant and animal cells have nuclei, which house just about all of the eukaryotic cell's DNA. D. Plant cells contain the organelles to carry out photosynthesis.
Plant cells do not have mitochondria.
Which of the following statements does NOT provide evidence for the endosymbiotic theory? A. The ribosomes contained within mitochondria and chloroplasts are very similar to prokaryotic ribosomes. B. Prokaryotes contain peptidoglycan in their cell walls. C. The same antibiotics that inhibit protein synthesis in prokaryotes also inhibit protein synthesis within mitochondria and chloroplasts. D. Mitochondria and chloroplasts contain circular DNA, similar to the DNA in prokaryotes.
Prokaryotes contain peptidoglycan in their cell walls.
Prokaryotic
Simple Prokaryotic or Eukaryotic
What will occur if a bacterial cell with a weakened or damaged cell wall is placed in pure water? A. The cell will form endospores. B. The cell will form a new cell wall. C. The cell will remain the same size. D. The cell will shrink. E. The cell will swell and burst
The cell will swell and burst
Which statement best describes what happens when a gram-positive bacterium is placed in distilled water and penicillin? A. No change will result; the solution is isotonic B. Water will move into the cell C. Water will move out of the cell D. The cell will undergo osmotic lysis E. Sucrose will move into the cell from an area of higher concentration to one of lower concentration
The cell will undergo osmotic lysis
What statement most accurately describes the structure of the plasma membrane? A. Peripheral proteins span the membrane. B. The plasma membrane is a phospholipid bilayer with the hydrophilic phosphate groups oriented toward the inside and outside of the cell. C. The lipid portion of the bilayer is hydrophilic. D. The plasma membrane is a phospholipid bilayer with the hydrophilic phosphate groups oriented toward one another.
The plasma membrane is a phospholipid bilayer with the hydrophilic phosphate groups oriented toward the inside and outside of the cell
The nucleus of a eukaryotic cell differs from the nucleoid of a prokaryotic cell in all of the following ways EXCEPT which one? A. The prokaryotic nucleoid usually contains a single circular chromosome, whereas the eukaryotic nucleus contains multiple linear chromosomes. B. The eukaryotic nucleus is surrounded by a nuclear envelope. There is no envelope surrounding the prokaryotic nucleoid. C. The prokaryotic nucleoid contains DNA combined with histones. Histones are lacking in the eukaryotic nucleus. D. The eukaryotic nucleus contains nucleoli. There are no nucleoli in the prokaryotic nucleoid.
The prokaryotic nucleoid contains DNA combined with histones. Histones are lacking in the eukaryotic nucleus.
Which of the following is not a distinguishing characteristic of prokaryotic cells? A. They usually have a single, circular chromosome. B. They lack membrane-enclosed organelles C. They have cell walls containing peptidoglycan D. Their DNA is not associated with histones E. They lack a plasma membrane
They lack a plasma membrane
Which of the following is false about fimbriae? A. They are composed of protein B. They maybe used for attachment C. They are found on gram-negative cells D. They are composed of pilin E. They may be used for motility
They may be used for motility
Endospores can remain dormant for thousands of years. Once this dormant structure is provided appropriate nutrients and growth conditions, it will germinate to form a viable bacterial cell.
True
The cell membrane is a fluid structure that allows membrane proteins to move freely.
True
The endosymbiotic theory states that eukaryotic organelles evolved from symbiotic prokaryotes living within other prokaryotes.
True
The following bacterial structures increase the likelihood that bacteria will be associated with disease: capsules, fimbriae, and LPS.
True
The plane in which a bacterial cell divides determines the arrangement of cells.
True
Prokaryotic
Very small, much smaller Prokaryotic or Eukaryotic
Which statement best describes what happens when a gram-negative bacterium is placed in distilled water and penicillin? A. No change will result; the solution is isotonic B. Water will move into the cell C. Water will move out of the cell D. The cell will undergo osmotic lysis E. Sucrose will move into the cell from an area of higher concentration to one of lower concentration
Water will move into the cell
You have isolated a cell with a peptidoglycan cell wall. What other structure can you safely assume the cell has? A. a mitochondrion B. a chloroplast C. a nucleus D. a plasma membrane
a plasma membrane
During endospore formation, ________. A. the only DNA that is copied contains the genes necessary for germination B. the cell is experiencing a great deal of metabolic activity C. a thick spore coat forms around the forespore before the endospore is released into the environment D. the population of bacterial cells doubles
a thick spore coat forms around the forespore before the endospore is released into the environment
A population of bacterial cells has been placed in a very nutrient-poor environment with extremely low concentrations of sugars and amino acids. Which kind of membrane transport becomes crucial in this environment? A. osmosis B. facilitated diffusion C. active transport D. simple diffusion E. pinocytosis
active transport
Bacillus anthracis causes the deadly disease anthrax. Organisms of the genus Bacillus may form endospores. This bacterium would be suitable for biological warfare because endospores ________. A. have a high concentration of calcium ions B. are difficult to stain C. are centrally located within the bacterial cells D. are multilayered structures E. are resistant to high temperatures, UV light, and desiccation
are resistant to high temperatures, UV light, and desiccation
Short, prokaryotic appendages made up of pilin are called pili. Special pili involved in the transfer of DNA from one cell to another are called ______.
conjugation pili
The nuclear area of the bacterial cell ________. A. has abundant histone proteins B. is connected to the endoplasmic reticulum C. contains many linear chromosomes D. is enclosed in a nuclear membrane E. contains the bacterial chromosome
contains the bacterial chromosome
The antibiotic amphothericin B disrupts plasma membranes by combining with sterols; it will affect all of the following cells except A. animal cells B. gram-negative bacterial cells C. fungal cells D. Mycoplasma cells E. plant cells
gram-negative bacterial cells
Penicillin specifically interferes with peptidoglycan synthesis. Which of the following cells is most likely to be damaged by penicillin? A. human cells B. yeast cells C. cells of archaea D. gram-positive bacterial cells
gram-positive bacterial cells
Chemotaxis refers to the ability of microorganisms to ________. A. move in a wavelike motion B. escape phagocytosis C. move toward or away from chemical stimuli D. attach to solid surfaces E. survive under adverse conditions
move toward or away from chemical stimuli
In a prokaryotic cell, all of the following are functions of either the fimbriae or pili EXCEPT __________. A. transfer of DNA B. movement C. formation of biofilms D. attachment to a surface
movement
Condensed regions of chromosomes in eukaryotes where rRNA is synthesized are called ______ .
nucleoli
Where is the genetic information of the cell stored? A. rough endoplasmic reticulum (ER) B. nucleus C. Golgi apparatus D. lysosomes E. smooth endoplasmic reticulum (ER
nucleus
Which of the following pairs is mismatched? A. metachromatic granules - stored phosphates B. polysaccharide granules - stored starch C. lipid inclusions - poly - b - hydroxybutyric acid D. sulfur granules - energy reserve E. ribosomes - protein storage
ribosomes - protein storage
One of the ways smooth endoplasmic reticulum (ER) differs from rough endoplasmic reticulum is that rough ER is covered by A. the extracellular matrix. B. the Golgi apparatus. C. mitochondria. D. the cytoskeleton. E. ribosomes.
ribosomes.
Which of the following pairs is mismatched? A. lysosome; digestive enzymes B. ribosomes; storage C. Golgi complex; secretion D. mitochondria; ATP production E. endoplasmic reticulum; internal transport
ribosomes; storage
Gram-negative cells contain a periplasmic space that is ________. A. filled with lysozyme B. a site of endocytosis C. rich in degradative enzymes D. abundant in photosynthetic pigments E. the site of protein synthesis
rich in degradative enzymes
Endoplasmic reticulum that has ribosomes attached to its outer surface is referred to as ______ .
rough ER
Consider a gram-positive cell in a hypertonic medium. If the peptidoglycan were damaged, the cell would _____.
shrink, plasmolyze, collapse
Where are lipids made in the cell? A. Golgi apparatus B. rough endoplasmic reticulum (ER) C. ribosomes D. smooth endoplasmic reticulum (ER) E. mitochondria
smooth endoplasmic reticulum (ER)
Assume you are growing a bacterial culture in a glucose medium. Your lab partner adds more glucose in hopes of speeding up the experiment. The bacteria suddenly die. The best explanation for this result is that ________. A. the bacteria could not use glucose B. the cells plasmolyzed C. osmotic lysis occurred D. glucose is toxic to bacteria E. the cells underwent sporulation
the cells plasmolyzed