Diagnostic Microbiology Exam 2- Enteric Pathogens & more tests

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LDC

+,V,+

trehalose

-,+,+

arabinose

-,+,-

Name the 4 species of Shigella grouped by major O antigens

1) A- Shigella dysenteriae 2) B- Shigella flexneri 3) C- Shigella boydii 4) D- Shigella sonneii

List the three common forms of Yersinia and their diseases,

1) Yersinia pestis- plague 2) Yersinia enterocolitica-Enlarged mesenteric lymph nodes 3)Yersinia pseudotuberculosis- Septicemia

What three results can an amino acid medium detect?

1) decarboxylation 2) deamination 3) no interaction

The pH indicator for XLD is

phenol red

The amino acids all use bromocresol purple as the pH indicator EXCEPT _________; it requires the addition of ________ to test for deamination

phenylalanine (PDA); FeCl3

Salmonella is LDC

positive

Uninoculated amino acid tests and alkaline amino acid tests are both

purple (acidic is yellow)

Describe a decarboxylation tube result

purple, alkaline butt, K/K

the pH indicator of LIA is

Bromocrescol purple

_________________________ is the pH indicator in LIA

Bromocresol purple

The pH indicator for Hektoen agar is

Bromthymol blue

media used to distinguish Yersinia from other Enterobacteriaceae

CIN

The sulfur source in XLD is

sodium thiosulfate

(T/F) Y. pestis can be motile

False

The primary plate for stool cultures are

Hektoen and XLD

Describe a test in which there is no interaction with the amino acid (but fermentation of glucose)

K/A

ate three tests that can be used to distinguish S. typhi from S. cholerasuis and S. paratyphi.

LDC, Trehalose, Arabinose

What does LIA stand for

Lysine Iron Agar

Group C Shigella

S. boydii

Group B Shigella

S. flexneri

Group D Shigella

S. sonnei

What pathogen did typhoid Mary have

Salmonella typhi (Typhoid fever)

Group a Shigella

Shigella dysenteriae

-Most common isolate in US of Shigella

Sonnei (D)

(T/F) Y. pseudo and Y. enters can be motile

True

What test would you use to distinguish Citrobacter and Salmonella?

Urea OR LDC

Describe a deamination tube result

acidic, yellow butt, red slant, R/A

Decarboxylation is (anaerobic/aerobic)

anaerobic

Yersinia enterocolitica mimics _____________

appendicits

Amino acid decarboxylation results in a change of pH to

basic

Safety pin staining is also called ________ staining and is characteristic of which species?

bipolar; Y. pestis

Salmonella colonies are ______ on HE

black

Yersinia colonies look like a ________ on blood agar

bullseye

The element of a CIN plate are

cefsulodin, irgasan, novobioicn, hbile salts and crystal violet

The malonate test is similar to which other biochemical test?

citrate

What does it mean if the results are K/K? K/A? R/A?

decarboxylation; no interaction w amino acids; deamination

The sulfur source in HE is

ferric salts

Shigella colonies are ______ on HE

green

Citrobacter is LDC

negative

Proteus mirabilis is indole _________

negative

Salmonella is urease

negative

Yersiniz pests is urease

negative

Morganella is citrate __________, Providencia is citrate ___________

negative, positive

(T/F) Yersinia is motile at 25 degrees Celsius

true

E. coli colonies are _______ on HE

yellow

Virulence factors of Salmonella

•Fimbriae •Ability to traverse intestinal mucosa •Enterotoxin production


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