Enzymes
What are some examples of biological enzyme?
-Anhydrase -Catalase -Amylase -Protease
What are the factors that affect enzyme actions?
-Temperature -pH -enzyme concentration -substrate concentration
What is the definition of substrate?
A molecule that attaches to the enzymes active site
What is the definition of active site?
A region on an enzyme that binds to a protein or other substance during a reaction
The enzyme in pineapple that dissolves protein is called bromelain. Write a word "equation" to explain the chemical reaction that took place in one of your petri-dish. Hint: Reactants Product
Bromelain + Jell-O = Amino Acids
Which type of pineapple in your experiment has active enzymes? How do you know?
Fresh pineapple, because it broke down the Jell-O
Is this chemical reaction an example of SYNTHESIS (building) or HYDROLYSIS (breaking down)?
HYDROLYSIS, because the pineapple juice breaks down the Jell-O
What is and induced fit?
Once the enzymes -substrate complex is formed, the enzyme changes its shape slightly to create a more secure bond.
What enzyme is found in pineapple?
Protease
Chemically speaking, what is gelatin made of (is it a carbohydrate, a protein, or a lipid)?
Protein
Knowing the results of your experiment, why does your tongue get sore when you eat too much raw pineapple? (HINT- what is your tongue made of?)
The bromelain breaks down the proteins in your tongue.
A substrate is the chemical that enzymes act on. What is the substrate in your experiment?
The substrate is the Jell-O.
What is the definition of Enzyme?
They are proteins that catalyze the rate of a chemical reaction
What are some effects on chemical bonds?
They bring reactants closer, chemical bonds are weakened, and reactions proceed faster
What are some effects on activation energy?
They speed up chemical reactions by decreasing the amount of activation energy required for a reaction
Are enzymes specific or Not specific?
enzymes and substrates are specific to each other
Are enzymes reusable or not reusable?
reusable
