ILE 3 post lab quiz
In a typical experiment, what is the negative control?
A sample that tests the good working order of the experimental system. You or others have experience with this sample, and you expect to see little to none of the measured effect if everything is working as anticipated.
In a typical experiment, what is the positive control?
A sample that tests the good working order of the experimental system. You or others have experience with this sample, and you expect to see the measured effect or significantly more of it if everything is working as anticipated.
To test this hypothesis, which of the following would make the best experimental group?
Cervical cancer cells + turmeric
Based on your evaluation of the "week 2" data, select the correct choice.
The mean % cell viability of the "cervical cancer cells + turmeric" sample is statistically equivalent to the mean % cell viability of the "cervical cancer cells + no treatment" sample.
To test the good working order of your experimental system (MTT assay), which of the following would make the best positive control? Hint--remember that the measured effect is a reduction in % cell viability.
Percent (%) cell viability
What is the dependent variable for this experiment?
Percent (%) cell viability
If the results of the experimental group (and control group, if applicable) are inconsistent with the prediction, you _______ the hypothesis and conclude that the hypothesis is ________
Reject, invalid
If your hypothesis is valid, then what is the prediction?
The mean % cell viability of the "cervical cancer cells + turmeric" sample will be statistically less than the mean % cell viability of the "cervical cancer cells + no treatment" sample.
Based on the "week 2" data presented in the graph, select the correct choice regarding the positive control and negative control.
The results are consistent with the prediction.
Based on the "week 1" data presented in the graph, select the correct choice regarding the positive control and negative control.
The results are inconsistent with the prediction meaning that the experimental system is not working as anticipated.
Based on the "week 2" data presented in the graph, select the correct choice regarding the experimental group and control group.
The results are inconsistent with the prediction.
What is the independent variable for this experiment?
Turmeric (presence or absence)
If the question is, "Does turmeric kill cervical cancer cells?", then which of the following would be the most reasonable hypothesis?
Turmeric kills cervical cancer cells
Based on the "week 2" data presented in the graph, select the correct choice regarding the positive control and negative control.
WRONG ANSERW -> . The mean % cell viability of the "cervical cancer cells + Moringa oleifera" sample is statistically greater than the mean % cell viability of the "cervical cancer cells + no treatment" sample.
Based on the "week 1" data presented in the graph, select the correct choice regarding the positive control and negative control.
WRONG ANSERW -> . The mean % cell viability of the "cervical cancer cells + turmeric" sample is statistically greater than the mean % cell viability of the "cervical cancer cells + no treatment" sample.
If the experimental system (MTT assay) is working as anticipated, then what is the prediction?
WRONG ANSERW -> . The mean % cell viability of the "cervical cancer cells + turmeric" sample will be statistically less than the mean % cell viability of the "cervical cancer cells + Moringa oleifera" sample.
To test the good working order of your experimental system (MTT assay), which of the following would make the best negative control? Hint--in this experimental design the negative control is the same as the control group
WRONG ANSERW -> cervical cancer cells + no treatment
Based on your evaluation of the "week 2" data, select the correct choice.
You can interpret and evaluate the results of the experimental group (and therefore, assess the validity of the hypothesis) since the experimental system (MTT assay) was working as anticipated.
Based on your evaluation of the "week 1" data, select the correct choice.
You cannot assess the validity of the hypothesis.
Based on your evaluation of the "week 1" data, select the correct choice.
You cannot interpret and evaluate the results of the experimental group (and therefore, cannot assess the validity of the hypothesis) since the experimental system (MTT assay) was not working as anticipated.
If the 95% confidence intervals of two samples overlap, then this suggests that the means of the two samples are ________ .
not statistically different
If the 95% confidence intervals of two samples DO NOT overlap, then this suggests that the means of the two samples are ________ .
statistically different
If the results of the positive control and negative control are consistent with the prediction, then you conclude that the experimental system _____ working as anticipated, and you ______ interpret and evaluate the results of the experimental group/assess the validity of the hypothesis.
was, can