Lab 7 Photosynthesis
what wavelength of light did the colors yellow and blue abosrb the best?
yellow- 400-420 blue- 620
At what wavelength was transmittance the greatest for each dye?
yellow- 580-620 blue- 460
what colors does the pigment Xanthophyll represent?
yellow- dull
what colors does the pigment carotene represent?
yellow- orange
What colors does the pigment Chlorophyll B represent?
yellow-green
what is the number spectrum for the VIBGYOR
380-760nm
Which pigment traveled the fastest? why did it travel faster than other pigments?
Carotene was the fastest, because it was the most non-polar
what are the types of pigments we learned in lab?
Chlorophyll B Chlorophyll A Xanthophyll Carotene
Which pigments are the most polar and the least polar (make list greatest to least)
Chlorophyll B +++ Chlorophyll A++ Xanthophyll + Carotene -
Which pigment traveled the slowest? why did it travel slower than the other pigments?
Chlorophyll B , was the slowest because it was the most polar and was attacted to the polar paper.
what is the relationship between color and absorbance? what color will blue and yellow not absorb?
If you see somthing that is blue or yellow that means it will NOT absorb those colors only relfect them, thats why we see them!
What is in the solvents? and what is the proportion?
Petroleum ether Acetone 90/10
which color on the VIBGHOR scale has the most energy and which one has the lowest?
V has the most, R has the least
What colors does the pigment Chlorophyll A represent?
blue- green
Which of the pigments was most soluble in the solvent? How do you know this?
carotene, it traveled the farthest.
What color pigments are actually present in the leaf?
cholorphyll A, chlorophyll B, xanthophyll, caerotene
what is a pigment?
color substance, any substance that can absorb, transmit, and reflect light.
When you look at the leaf that was used for the chromatography experiment, what color pigment can you see?
green
What is the reason for calculating an Rf value for each pigment?
if someone asks you to identify the pigments in an unknown solution, you would be able to identify the pigments. Due to the fact that the polarity and how fare it moved will give away which on is which by looking and comparing the distance traveled.
how does light travel?
in waves
What is the Rf value?
it is defined as the ration of the distance moved by the solute ( ie the dye or pigment under test) and the distance moved by the solvent ( know as the Solvent Front) along the paper.
why is chlorophyll important in photosynthesis?
it is important because it is the reaction center
is petroleum ether acitone polar or non polar?
it is non- polar
what is a trough?
it is the lowest point the wave sinks too
If you had a greater concentration of dye in the tube did it increase the light abosrption or decrease it?
it would increase it, the greater the concentration of dye in the tube, the greater the light absorption
when it comes to polar or non- polar which combination dissolves each other if put together?
like dissolves like
Which molecules will be carried up the paper futher than others and why?
molecules with low polarity, due to the fact that the paper is polar and the molecules are also polar they will come together and star dissolving. And there for it will stay at the certain place and not move up. BUT its something does not have a high polarity it will not bond with the paper and will be able to freely climb up the paper to the other non-polar substance.Basically the "like" polar or non-polars want to be together.
are all wavelengths the same size?
no some are shorter or longer than others
what causes the separation of colors on the paper?
paper chromatography
is paper polar or non-polar?
polar
What do you need to make energy from glucose
the green pigment chlorophyll
what is a crest?
the highest point the wave gets too
what is wavelength?
the length of space from one crest to another crest (from B to F)
which wavelength has the most energy? a long one or a short one?
the shorter the wavelength the higher the energy
