Lab 9: Bacteria Flagella & Motility Testing

Amphitrichous

flagella at both ends of the cell

peritrichous

flagella that cover the surface of a cell

why do we study the different arrangements of flagella

knowing the presence of flagella and their arrangements helps identify and classify bacteria

What is atrichous?

no flagella

Lophotrichous

several flagella at one end

Monotrichous

single flagellum

motility test medium method

-uses Tri Tetrazolium chloride (TTC) a colorless dye -it is converted to purple only by live bacteria -if the purple is seen to diffuse away from the stab line than it is motile

How do we study the different arrangements of flagella

1. Stain bacteria for flagella 2. Hanging drop technique 3. Motility test medium method

What are the 3 units bacterial flagella is composed of

Filament, Hook, Basal body

What are the different arrangements of Flagellla

Monotrichious, Amphitrichous, Lophotrichous and Peritrichous

Brownian movement

erratic movement of any microscope particles

hanging drop technique

examines live, unstained , small organisms shows Brownian movement