LabCE Lab Ops

An aqueous 2.00 M HCl solution is prepared with a total volume of 0.475 L. The molecular weight of HCl is 36.46 g/mol. What mass of hydrochloric acid (in grams) is needed for the solution?

34.6 g

A completed package for a category B substance must be able to survive a drop test from what height?

4 feet

What do you need to make up 450 mL of a 3.8 M solution of NaOH (gmw=39.997 g/mol)?

68.4 g NaOH

What is the coefficient of variation for this creatinine testing method? Use (Mean = .97 mg/dL, standard deviation = .07 mg/dL)

7.22%

Your laboratory has 17,264 hours of total paid labor time. Of this amount, 1458 hours are nonproductive. If your employees work 40-hour work weeks each year, what is the total number of FTEs (full-time equivalents) needed to run the laboratory?

8.3

What is the Coefficient of Variation for a set of numbers when: ± 1 SD = 6.8, mean = 80, and the range for ± 1 SD = 73.2 - 86.8?

8.5%

What is the normality of 32.1% HCl? (HCl has a positive valence of 1)

8.8

Which of the following items must be labeled with a biohazard label or be colored red or orange to indicate it is a biohazard?

A container that is used for disposal of needles in the blood collection area.

Which of the following best defines "specificity":

A test's ability to correctly exclude individuals who do not have the given disease or disorder.

All of the following are directly responsible for setting and monitoring competency requirements for laboratory personnel, EXCEPT:

CLSI

EDTA and Sodium citrate combine with _________ to inhibit coagulation in blood samples.

Calcium

In the clinical laboratory, after correctly matching the blood collection tube(s) with the appropriate test requisition and patient identification labels, the laboratorian will ascertain if the sample is acceptable for further processing. What is NOT part of this criteria?

Checking for hemolysis.

What type of policy is required by OSHA for every laboratory that uses hazardous chemicals?

Chemical hygiene plan

All outpatient tests are reimbursed according to a group of code numbers which are called:

Current Procedural Terminology (CPT) Codes

A common condition among clinical laboratorians, all of the following are signs or symptoms of eyestrain, EXCEPT?

Increased ability to focus between images on and off the screen

The risk of laboratory-acquired infections with viruses, such as HIV-1, is a critical consideration. All of the following routes are considered modes of transmission with infected blood or other body fluids, EXCEPT?

Inhalation

A nucleic acid used to identify a hybridization target is termed:

Oligonucleotide

Which of the following describes the Westgard multi-rule R4S?

One data point is +2SD and a consecutive data point is -2SD

An electrode has a silver/silver chloride anode and a platinum wire cathode. It is suspended in KCl solution and separated from the blood to be analyzed by a selectively permeable membrane. Such an electrode is used to measure which of the following?

PO2

All of the following are examples of Protected Health Information (PHI), EXCEPT?

Patient education level

Which integral member of the laboratory team plays a vital role in minimizing pre-analytical variability associated with the clinical laboratory testing process?

Phlebotomist

Which type of error is associated with the precision of the method?

Random error

Which of the following evaluation tools for documenting direct observations is the least subjective?

Rating scale with detailed descriptions of traits

The Lean approach seeks to streamline the process. What is the major measurement for Lean?

Time

In the steps involved in performing a bone marrow differential what objective is used and how many cells must be counted?

100x (oil), 200 cells

A 24-hour urine collection requires HCl as a preservative. The procedure manual states the required concentration of HCl in percent rather than in normality. The laboratory only has 4N HCl available. What is the percent concentration? (HCl has a positive valence of 1)

14.6%

Your community hospital is considering the addition of a new hematology screening test. The laboratory is asked to calculate the total cost of quality control per new test. Quality control must be performed 3 times per day (every 8 hours). The labor cost per quality control test is $8.95 each. A day's worth of quality control reagent costs $23.62. What is the total quality control cost per new hematology test if 6,700 of these new tests are performed each year?

2.75

A technologist put five (5) tubes in a test tube rack and put 3 mL of diluent in each tube. The tech then put 0.3 mL serum in the first tube and mixed it. Next, the tech then transferred 1 mL of the mixture from the first tube to the second tube. This process was carried on through tube number five (5). What is the dilution factor in tube number five?

2816

Which of the following analytes would be increased due to a delay in centrifugation?

ALT

The traditional Polymerase Chain Reaction (PCR) procedure has three important functions and include all of the following EXCEPT:

Amplifies RNA

Which of the following statements best describes the mean and median as they relate to a normal "Gaussian" distribution curve?

Both are equal

Which of the following identifies a major mechanism of electricity-induced injury?

Direct trauma resulting from falls or violent muscle spasms

Which of the following is considered an indirect hazard of electrical energy?

Fire

When quantifying glucose using an amperometric glucose electrode system, all of the following are components of the system, EXCEPT?

Hexokinase reacts with glucose

An effective voluntary accreditation agency for a clinical laboratory has a role in all of the following areas EXCEPT:

Hiring and dismissal of employees.

Which of the following is the main function of the ASCP Board of Certification?

Maintain a registry of certified personnel

All of the following are considered repetitive strain disorders, EXCEPT?

Rheumatoid arthritis

A spill of a chemical listed as a potential carcinogen has occurred in the laboratory. What document should the laboratory personnel use to find information on how to handle the spill?

Safety Manual

Mercury covered by a layer of mercurous chloride in contact with saturated potassium chloride solution describes which of the following types of electrodes?

Saturated Calomel

To prevent or inhibit coagulation of blood in collected samples, the use of the following anticoagulants and/or additives are recommended EXCEPT:

Sodium polyanethol sulfonate

What does the equation in parenthesis measure? (TN/TN+FP)

Specificity

If a commercial lyophilized reagent is diluted using slightly more distilled water than indicated, what type of error will occur?

Systematic error

All of the following test principles are matched with the most appropriate response, EXCEPT:

TMA - Target amplification using reverse transcriptase. Thermal cycling is needed.

What are the two general categories of nucleic acid amplification (NAA) techniques?

Target amplification and signal amplification

The use of objective tests for evaluating clinical performance is limited because they:

Tend to measure only cognitive abilities

In which section of the safety data sheet (SDS) would you find information about whether a specific chemical is a carcinogen?

Toxicological information section

Which of the following best defines "effective clinical laboratory services?"

Using evidence to identify the best test to detect or diagnose a condition

The majority of laboratory errors occur during the preanalytic phase of testing. Which of the following would be a preanalytic error related to laboratory testing?

Wrong test ordered

What is the basis for many types of analyses used in the clinical laboratory, such as potentiometry, amperometry, coulometry, and polarography?

electrochemistry

Which of the following is a direct nucleic acid test?

fish

Which of the following analytes would be decreased due to delay in centrifugation?

folate

What is a workload reporting system, and why is it an important part of laboratory management?

helps in planning, developing, and maintaining efficient laboratory services with administrative and budget controls

A CLIA waived test requires that operators:

must follow manufacturer's instructions

A tool used to show chain of command by defining responsibility and authority is a/an:

organizational chart

The degree to which a measure is consistent in producing the same results when measuring the same analyte is called:

reliability

Which of the following must be included as part of a PCR in order to quantify unknown samples?

standards