Media lab

After experiencing extreme fatigue and polyuria, a patient's basic metabolic panel is analyzed in the laboratory. The result of the glucose is too high for the instrument to read. The laboratorian performs a dilution using 0.25 mL of patient sample to 750 microliters of diluent. The result now reads 325 mg/dL. How should the techologist report this patient's glucose result? Please select the single best answer 325 mg/dL 1300 mg/dL 975 mg/dL 1625 mg/dL

1300 mg/dL

Approximately what percent of the overall population is Rh negative: Please select the single best answer 10 % 15 % 25 % 35 %

15 %

Which of the following characteristics should be observed for and noted in the macroscopic examination of a stool sample for parasites? More than one answer is correct. Please select all correct answers Color Consistency Time that last meal occurred Gross appearance

Color Consistency Gross appearance

The radioactive method used to measure red cell survival uses which of the following isotopes: Please select the single best answer I125 Cr51 P31 C14

Cr51

Which of the following blood components contain the most factor VIII concentration relative to the unit volume? Please select the single best answer Plasma Cryoprecipitated AHF Fresh Frozen Plasma Platelet Concentrate Whole blood

Cryoprecipitated AHF

he image on the right contains the fluorescence data from CD45 from a flow cytometry analysis. Note that virtually all cells in the gated population have CD45 surface antigens, indicating they are which type of cells? The correct answer is highlighted below Leukocytes Erythrocytes Platelets

Leukocytes

A patient with a Platelet count of 230 x 109/L, and a Bleeding time of 15 minutes, could be suffering from which condition? Please select the single best answer Defective platelet function Decreased platelet production Increased platelet production Increased platelet destruction

Defective platelet function

Which of the following tests has been recommended by the FDA to replace the HIV-1 p24 antigen test in the screening of donated blood for infectious diseases? The correct answer is highlighted below HIV-1 NAT Anti-HIV-1 Anti-HIV-2 HCV NAT

HIV-1 NAT

Patients with Cushing syndrome exhibit: Please select the single best answer Decreased plasma 17-hydroxysteroid concentration Decreased urinary 17-hydroxysteroid excretion Serum cortisol concentrations greater than 15 mg/dL Decreased cortisol secretion rate Decreased ACTH levels

Serum cortisol concentrations greater than 15 mg/dL

Plasmodium falciparum gametocytes

Serum sickness Arthus reaction Tuberculin reaction

What type of follow-up testing is recommended for a Speckled ANA pattern? Please select the single best answer None Testing for antibodies to the extractable nuclear antigens (ENA) Testing for antibodies to dsDNA

Testing for antibodies to the extractable nuclear antigens (ENA)

This parasite was recovered in a veterinary stool sample. Which of the following human parasites does it most closely resemble. Please select the single best answer Trichinella spiralis encysted larva Ascaris lumbricoides egg Enterobius vermicularis egg Trichuris trichiura egg

Trichuris trichiura egg

A rosette test to screen for FMH is contraindicated if the newborn is weak D. The correct answer is highlighted below True False

True

All of the species listed below produce microconidia that aggregate in clusters: Trichoderma species Acremonium species Fusarium species Gliocladium species The correct answer is highlighted below True False

True

HbA1C measurements are NOT ordinarily used to monitor long-term diabetic control in a diabetic with sickle cell anemia. Select true or false True False

True

Hydrogen ion concentration (pH) in blood is usually determined by means of which of the following electrodes? Please select the single best answer silver glass platinum gold

glass

A patient has the following test results: ANA = positive 1:320 RA = positive Complement = decreased The above results would be seen in patients with: Please select the single best answer rheumatic fever rheumatoid arthritis lupus erythematosus glomerulonephritis lyme disease

lupus erythematosus

A specimen drawn from an indwelling catheter that was contaminated by heparin would be indicated by: Please select the single best answer normal PT and aPTT results abnormal PT and normal PTT results prolonged aPTT and prolonged reptilase time test prolonged aPTT and normal reptilase time test

prolonged aPTT and normal reptilase time test

Given the following results, what is the immune status of the patient? HbsAg: negative HbeAg: negative Anti-Hbc: negative anti-HBs: positive anti-HBe: positive The correct answer is highlighted below acute infection chronic infection recovery immunization

recovery

Which of the following crystals will NOT normally be found in a urine specimen with a pH of 8.0? Please select the single best answer ammonium biurates triple phosphates calcium carbonates uric acid no crystals appear at that high pH level

uric acid

Match the descriptions with the cells. Select the correct match for each item from the drop-down box Pale blue cytoplasm. Nucleus may be oval or indented. Blue-gray opaque cytoplasm. Nucleus may have convolutions. Light pink cytoplasm with fine granules. Nucleus with 2-5 lobes. Colorless cytoplasm with large red refractile granules.

Lymphocyte: Pale blue cytoplasm. Nucleus may be oval or indented. Monocyte : Blue-gray opaque cytoplasm. Nucleus may have convolutions. Segmented Neutrophil: Light pink cytoplasm with fine granules. Nucleus with 2-5 lobes. Eosinophil: Colorless cytoplasm with large red refractile granules.

The recommended storage temperature for the preservation of activity for MOST enzymes is: Please select the single best answer 0 ºC 4 ºC 25 ºC 37 ºC

4 ºC

What percentage of glycerol is generally used when freezing red cells of rare phenotypes: Please select the single best answer 70 % 40 % 10 % 5 %

40 %

In order to prevent a loss of viability in platelet concentrates during storage the pH must be maintained above: The correct answer is highlighted below 7.5 6.2 5.0 4.5

6.2

A 1:100 dilution of a patient peripheral blood was made and 136 Platelets were counted in 5 small RBC counting blocks of a Neubauer-ruled hemocytometer. What is the Platelet count? Please select the single best answer 27 x 103/µL 68 x 103/µL 136 x 103/µL 680 x 103/µL 13.6 x 103/µL

680 x 103/µL

If 100% of the cells in the gated lymphocyte population are positive for CD45 (i.e., they are leukocytes) and 7% are B cells (represented by CD19 and CD20 positivity), approximately what percentage of T cells would be expected? Please select the single best answer 10% 50% 93% It's impossible to predict

93%

Which of the following terms may be defined as the extent to which a measurement agrees with the true value of the analyte being measured? Please select the single best answer Reproducibility Accuracy Precision Linearity

Accuracy

Glycoproteins

Albumin

The most important bloodborne pathogens are: Please select the single best answer Hepatitis B Hepatitis C HIV All of the above

All of the above

Which listed transfusion reaction is MOST OFTEN associated with transfused patient's lacking IgA? Please select the single best answer Hemolytic Anaphylaxis Febrile Transfusion-associated circulatory overload (TACO)

Anaphylaxis

A technologist performing an antibody identification in the blood bank has recorded the following results found in the worksheet below. Which antibody(ies) listed below cannot be ruled out? The correct answers are highlighted below Anti-N Anti-C Anti-k Anti-e Anti-E Anti-c Anti-K

Anti-C Anti-E Anti-K

What is the MOST likely explanation for a patient with sickle cell anemia whose hemoglobin has dropped to 4 g/dL and has a reticulocyte count of 0.1% Please select the single best answer Increased hemolysis due to hypersplenism Aplastic crisis Thrombotic crisis Occult blood loss

Aplastic crisis

All of the following are benefits of autologous donation except: Please select the single best answer Reduces exposure to infectious agents Are always on hand in case of an unexpected emergency Reduces demand for homologous blood Eliminates sensitization to cellular blood components

Are always on hand in case of an unexpected emergency

Match the organism to the disease produced outside a bioterrorism event. Select the correct match for each item from the drop-down box Anthrax Pneumonic plague Melioidosis Brucellosis Tularemia Glanders

Bacillus anthracis Anthrax Yersinia pestis Pneumonic plague Burkholderia pseudomallei Melioidosis Brucella species Brucellosis Francisella tularensis Tularemia Burkholderia mallei Glanders

Which of the following is not a component of a basic single beam spectrophotometer: Please select the single best answer Beam splitter Cuvette Prism Light source

Beam splitter

When should you first refer to a chemical's label? Please select the single best answer Before moving or opening the chemical Before disposing of the chemical After a spill After accidentally ingesting the chemical

Before moving or opening the chemical

Which of the following are the beta thalassemia syndrome(s) whose genotype is B0/B+? More than one answer is correct. Please select all correct answers Beta thalassemia silent carrier Beta thalassemia minor Beta thalassemia intermedia Beta thalassemia major

Beta thalassemia intermedia Beta thalassemia major

Streptococcus pnuemoniae can BEST be differentiated from the viridans group of streptococci by which characteristic? Please select the single best answer Gram stain The type of hemolysis Bile solubility Colonial morphology

Bile solubility

Bacterial contamination of a urine specimen from a normal healthy individual could originate from all of the following except: Please select the single best answer Contaminated urine container Vagina Bladder Vulva

Bladder

Serum alkaline phosphatase activity is derived from all of the following organs except: Please select the single best answer Bone Intestine Placenta Brain

Brain

Which of the following is used as an indicator of inflammation? Please select the single best answer C-Reactive Protein (CRP) RPR Complement Alpha fetoprotein

C-Reactive Protein (CRP)

An increase in CEA levels is most closely associated with which of the following organs: Please select the single best answer Heart Bone Colon Liver

Colon

All of the following concerning CK-MB (CK-2) are true EXCEPT: The correct answer is highlighted below Greater than 5% or 10 IU/L followed by an LD-1: LD-2 flip is specific evidence of myocardial infarction (MD). CK-MB levels can be normal with an elevated total CK. CK-MB and troponin levels can be elevated after an AMI. CK-MB levels are normal in cardiac ischemia.

CK-MB levels are normal in cardiac ischemia.

A stool from a two-year-old girl grew clear colonies on MacConkey agar, red colonies on XLD, and green colonies on HE without black centers. It was ornithine positive and indole negative. It should agglutinate in antisera of which serogroup? Please select the single best answer A B C D

D

When are data collected in a real-time PCR procedure? Please select the single best answer Prior to addition of fluorescent dyes or probes At a single endpoint During nucleic acid amplification

During nucleic acid amplification

Granulocytes include neutrophils, basophils and platelets. The correct answer is highlighted below True False

False

The ADA recommends performing an oral glucose tolerance test (OGTT) on all individuals who are at increased risk for diabetes. The correct answer is highlighted below True False

False

Which of the following is found in plasma but absent in serum: Please select the single best answer Proteins Fibrinogen Red blood cells White blood cells

Fibrinogen

Match each parasite listed here with its corresponding infective stage: Select the correct match for each item from the drop-down box Hookworm Taenia solium Trypanosoma cruzi Schistosoma mansoni

Filariform larva :Hookworm Egg Taenia solium Trypomastigote Trypanosoma cruzi Cercaria Schistosoma mansoni

Which of the following serum constituents is unstable if a blood specimen is left standing at room temperature for eight hours before centrifugation and processing? Please select the single best answer Cholesterol Triglyceride Creatinine Glucose

Glucose

Acute phase proteins generally fall into which category? Please select the single best answer Glycoproteins Immunoglobulins Lipoproteins Microglobulins

Glycoproteins

Which of the following is the most probable explanation for the results below? The following results were obtained: ABO/Rh Type DAT Ab. Screen and ID Mother A negative NT Positive anti-D Infant O positive 4+ IgG NT NT = not tested Please select the single best answer Hemolytic disease of the newborn due to antibody against a high frequency antigen Baby has an autoimmune syndrome Hemolytic disease of the newborn due to anti-D

Hemolytic disease of the newborn due to anti-D

Which one of the following immunoglobulins is MOST likely to be elevated in anaphylactic shock? The correct answer is highlighted below IgA IgD IgE IgG IgM

IgE

Which one of the following combinations of organisms would be appropriate positive and negative controls to verify the specific test functions listed? Please select the single best answer Beta hemolysis - Escherichia coli and Streptococcus pyogenes Catalase - Staphylococcus aureus and Staphylococcus epidermidis Hydrogen sulfide production - proteus mirabilis and Salmonella typhi Indole - Escherichia coli and Klebsiella pneumonia Carbohydrate fermentation - Alcaligenes faecalis and Acinetobacter baumannii

Indole - Escherichia coli and Klebsiella pneumonia

Sodium fluoride is an excellent preservative for collecting glucose specimens because: Please select the single best answer Prevents interference with other substances Inhibits glycolysis Enhances chemical reactions Inhibits certain clotting factors

Inhibits glycolysis

I measure 12 µm and am found in stool. The correct answer is highlighted below Pneumocystis carinii cyst Entamoeba coli cyst Endolimax nana cyst Iodamoeba butschlii cyst

Iodamoeba butschlii cyst

Isoenzymes of alkaline phosphatase occur in: The correct answer is highlighted below Kidney and bone Bone and brain Liver and pancreas Brain and liver

Kidney and bone

On an electronic cell counter, hemoglobin determination may be falsely elevated caused by the presence of: Please select the single best answer Lipemic or icteric plasma Leukocytopenia or Leukocytosis Rouleaux or agglutinated RBCs Anemia or Polycythemia

Lipemic or icteric plasma

A college student is treated in the hospital for suspected meningitis. Which cells should be identified in the cerebrospinal fluid report for the cytospin field that is shown? More than one answer is correct. Please select all correct answers Monocytes Blast cells Lymphocytes Tumor cells Atypical lymphocytes

Lymphocytes Atypical lymphocytes

An increase in the predominant white cell type

Lymphocytosis

Which of the following tests could be used to determine whether an abnormal screening coagulation test result (PT or aPTT) is caused by a factor deficiency or an inhibitor?. Please select the single best answer Bleeding time Mixing studies Fibrin split products (FSP) Fibrinogen assay

Mixing studies

The numeric value that occurs most frequently in a sample distribution is called the: Please select the single best answer Coefficient of Variation Mean Median Mode Standard Deviation

Mode

If 100% of the cells in the gated lymphocyte population are positive for CD45 (i.e., they are leukocytes) and 7% are B cells (represented by CD19 and CD20 positivity), approximately what percentage of T cells would be expected? Please select the single best answer 10% 50% 93% It's impossible to predict

Nephrotic syndrome

Which statement best describes the relationship between dietary iron and iron absorption in a healthy individual? Please select the single best answer Only a small percentage of dietary iron is absorbed and used. All dietary iron is needed for hemoglobin and therefore absorbed. Most people need to take dietary iron supplements to meet their needs for iron. All dietary iron is absorbed and excess is stored.

Only a small percentage of dietary iron is absorbed and used.

Which of the following might cause a false positive indirect antiglobulin test: The correct answer is highlighted below Failure to adequately wash cells Delay of addition of antiglobulin reagent. Overcentrifugation High concentration of IgG paraprotein in a patient's serum

Overcentrifugation

Which of the following parasites is/are most commonly recovered in sputum samples? Please select the single best answer Onchocerca volvulus Taenia saginata scolex Diphyllobothrium latum scolex Paragonimus westermanni

Paragonimus westermanni

A physician is evaluating a 45-year-old obese male for diabetes and orders a plasma glucose at time of evaluation and a HbA1C one week later. The patient has a family history of diabetes and currently exhibits symptoms of diabetes. What would be the best course of action if these are the blood glucose results? Casual Blood Glucose: 208 mg/dL HbA1C one week later: 7.2% Please select the single best answer Order insulin levels; if decreased, diagnose diabetes Patient meets criteria for diagnosis of diabetes Order a C-peptide for diagnosis The patient is at risk for diabetes (pre-diabetic); monitor for diabetes with yearly FPG levels

Patient meets criteria for diagnosis of diabetes

Which animal was involved in the reassortment of influenza A viruses that led to the 2009 novel influenza A H1N1 virus? Please select the single best answer Cows Deer Horses Pigs

Pigs

I reside inside red blood cells, where I grow and grow until the cells are eventually destroyed. Please select the single best answer Plasmodium falciparum gametocytes Plasmodium ovale gametocytes Plasmodium malariae gametocytes Plasmodium vivax gametocytes

Plasmodium falciparum gametocytes

Which of the following parasites may be recovered in the peripheral blood? The correct answers are highlighted below Plasmodium vivax Leishmania donovani Trichinella spiralis Wuchereria bancrofti

Plasmodium vivax Leishmania donovani Wuchereria bancrofti

A urine specimen which was collected in a dark container, stored in the dark, has a pH of 7.5, and whose sample aliquot is wrapped in foil, is most likely being sent for: Please select the single best answer Aldosterone Porphyrins Catecholamines Metanephrines

Porphyrins

Given the following commonly used nomenclature systems, which one of the Rh genotypes listed below is heterozygous for the C antigen? WienerFisher-Race (haplotype) RoDce R1 DCe R2DcE RzDCE rdce r'dCe r"dcE rydCE The correct answer is highlighted below R1r R1R1 R2r" r"r

R1r

For a substance to be immunogenic it must be: Please select the single best answer A linear molecule A lipid Less than 5,000 molecular weight Recognized as non-self A haptene

Recognized as non-self

In the Coombs phase of a crossmatch, what is the proper procedure to follow if the Check Cells give a negative reaction? Please select the single best answer Repeat procedure with new AHG reagent and check the cell washer Add additional check cells and dilute with 100% distilled water Add additional AHG reagent plus proteolytic enzymes to enhance reaction Record all results as negative and hope no one catches the mistake Accept the cross match results since nothing is wrong.

Repeat procedure with new AHG reagent and check the cell washer

According to the National Diabetes Data Group recommendations, which of the following statements is TRUE with regards to the following findings? Fasting glucose = 130 mg/dl 2-hour post prandial glucose = 210 mg/dl Please select the single best answer The patient may be diagnosed as having diabetes mellitus The patient is diagnosed as having impaired glucose tolerance The patient is diagnosed as having diabetes insipidus The patient is normal

The patient may be diagnosed as having diabetes mellitus

The preparation of a Levey-Jennings quality control chart for any constituent of serum requires: Please select the single best answer a minimum of 20-30 analyses of control serum over a period of 20-30 days. 20-30 analyses of control serum on one day, one batch. daily analyses for 1 month with the use of aliquots from more than one lot control serum. 20-30 analyses performed by one person. weekly analysis of the control serum for one month.

a minimum of 20-30 analyses of control serum over a period of 20-30 days.

When the body compensates for a respiratory or metabolic disorder, the MAIN goal is to achieve: Please select the single best answer correct carbonic acid level, since it is the most important system in the body a normal pH normal p02 and pCo2 for normal respiration correct bicarbonate level, since it is the most important system in the body

a normal pH

The type of health-care occupational exposure with the greatest risk of HIV transmission is: Please select the single best answer a percutaneous injury mucous membrane exposure abraded skin exposure

a percutaneous injury

B-hydroxybutyric acid is formed as the result of an accumulation of which of the following? Please select the single best answer acetyl Co A oxaloacetic acid uric acid alpha-hydroxybutyric acid

acetyl Co A

Which of the following cells are considered abnormal and clinically significant on a CSF differential? More than one answer is correct. Please select all correct answers ependymal blast pia arachnoid mesothelial malignant

blast malignant

Match the technique to the adverse effect it has on a capillary specimen Select the correct match for each item from the drop-down box Failure to mix a specimen containing an anticoagulant by inverting the sealed specimen container several times after collection of the specimen Puncture of the cleansed site before alcohol is completely dry Not wiping away the first drop of blood prior to specimen collection Holding the hand from which blood is being collected in an upward position during collection of the specimen.

clots in specimen: Failure to mix a specimen containing an anticoagulant by inverting the sealed specimen container several times after collection of the specimen hemolysis: Puncture of the cleansed site before alcohol is completely dry Specimen contaminated or diluted with tissue fluid: Not wiping away the first drop of blood prior to specimen collection decreased blood flow: Holding the hand from which blood is being collected in an upward position during collection of the specimen.