Molecular Genetics Chapter 17
Which of the following is not key to performing the Polymerase Chain Reaction® for the amplification of a piece of DNA. a. A single stranded denatured DNA "template" b. 2 specific DNA primers c. A mixture of four deoxynucleotides and one dideoxynucleotide d. A DNA polymerase e. Repeating the temperature cycles many times
A mixture of four deoxynucleotides and one dideoxynucleotide
Southern blots probe
DNA
________ seals a nick in the DNA by creating a sugar phosphate bond between the adjacent disjointed nucleotides.
DNA ligase or topoisomerase
In each cycle of PCR what is the order of the steps (i.e. first, second and third)
Denaturation, primer annealing, extension
The methodology to clone animals such as sheep outlined in this article is essentially the same as the technology to clone DNA described earlier in the chapter.
False
Which of the following is not an essential feature or function of a plasmid cloning vector?
Origin of transfer (oriT)
cDNA can be synthesized from mRNA by using the following enzyme
Reverse transriptase
Which of the following is not an essential feature or function of a bacterial plasmid cloning vector?
Screenable marker (e.g. lacZ)
Restriction enzymes that cut between different nucleotide pairs within a palindrome on complementary DNA stands produce _______ ended DNA fragments
Sticky
Automated sequencing differs from manual sequencing in that automated sequencing:
Uses dideoxynucleotides labeled with fluorescent dyes
The technique that uses antibodies to detect proteins on a blotted gel is called a....
Western Blot
A "probe" in molecular biology is __________.
a DNA or an RNA molecule used in hybridization reactions
An E. coli plasmid used as a cloning vector must have
a bacterial origin of replication, unique restriction sites, and a selectable marker
Yeast Artificial chromosomes must have ____ to permit them to replicate in an eukaryotic host cell:
a centromere
Which is the most specific recombinant DNA library?
cDNA
In nature, the function of restriction enzymes is to
destroy phage DNA
At the end of each cycle the temperature of the PCR reaction is raised to 72°C in order to _____
elongate the primer
In the manual Sanger sequencing (chain termination), _______ sequencing reaction(s) is(are) performed.
four
For PCR, DNA can be isolated from the following source: ___.
frozen tissue, spermatic cells, fossil specimens, and preserved tissue
Recombinant DNA research is dependent on
hosts, cloning, restriction endonucleases, and vectors
Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally
hybridizes filter-bound DNA with a DNA probe.
You want to clone a fragment of DNA into a plasmid vector such as pUC18. The plasmid is 4.36 kb long and has a single restriction site for the restriction enzyme BamHI. If you digest pUC18 with BamHI, how many linear fragments will remain?
one
Western blots detect specific ____________ in a sample
proteins
Restriction enzymes cut DNA at specific palindromic segments of DNA referred to as
recognition sites
The polymerase chain reaction is a technique that __________.
selectively replicates DNA
Restriction endonucleases are especially useful if they generate "sticky" ends. What makes an end "sticky"?
single-stranded complementary tails
Which of the following DNA restriction fragments would travel the farthest distance through an agarose gel ? (if run on the same gel at the same time)
smallest: 0.5 kb
You want to close a fragment of DNA into a plasmid vector such as pUC18. The plasmid is 5 kb long and has two restriction sits for the restriction enzyme BamHI. If you digest pUC18 with BamHI, how many linear fragments will result?
two
A "YAC" is a useful
vector