Pre Class 4: Transcription

Choose all that apply: Which of the following statements correctly describes the role of transcription factors in the initiation of transcription? (A) Some transcription factors bind to both strands of the DNA, whereas others bind specifically to either the template or coding strand. (B) Some transcription factors must bind to the promoter before RNA polymerase II can bind. (C) Binding of a transcription factor to DNA requires a specific sequence of bases in the DNA binding region. (D) Binding of transcription factors to the DNA causes the DNA to unwind, exposing the template strand to RNA polymerase II.

(A) (B) and (C)

Does RNA polymerase move in a set direction along a strand of DNA during transcription? (A) Yes; the RNA polymerase moves in a direction that reads the bases of the DNA sequence from the 3' end toward the 5' end. (B) No; the RNA polymerase can move in either direction along the DNA strand because the same sequence of bases in the RNA will be produced regardless of direction. (C) Yes; the RNA polymerase moves in a direction that reads the bases of the DNA sequence from the 5' end toward the 3' end.

(A) Yes; the RNA polymerase moves in a direction that reads the bases of the DNA sequence from the 3' end toward the 5' end.

Which of the following *initially* determines which DNA strand is the template strand, and therefore in which direction RNA polymerase II moves along the DNA? (A) the specific sequence of bases along the DNA strands (B) the position of the gene's promoter on the chromosomes (C) the location of specific proteins (transcription factors) that bind to the DNA (D) the location along the chromosome where the double-stranded DNA unwinds (E) which of the two strands of DNA carries the RNA primer

(A) the specific sequence of bases along the DNA strands

Which two statements correctly describe the template and/or coding strands? (A) The four types of bases that are used in the coding strand are different from the types that are used in the template strand. (B) The template and coding strands are always antiparallel; that is, if one strand has its 3' end on the left and its 5' end on the right, the other strand has the opposite orientation. (C) During transcription, the DNA bases on the template strand are paired with their complementary RNA bases to form the RNA transcript. (D) As the RNA polymerase moved along the DNA, the DNA bases on the coding strand are copied, producing the identical sequence of bases in the RNA transcript.

(B) and (C)

True or False: Transcription factors function to help move ribosomes along the mRNA.

False