Test 1 Micro bio Lab
The coarse and fine focus knobs adjust the distance between: the stage and the condenser lens the stage and the objective lens the objective and ocular lenses the ocular lenses and your eyes the ocular lenses
the stage and the objective lens
Which items can be discarded in a regular trash can? (Check all that apply) paper towels used to wipe up bacterial spills uncontaminated waste paper wrappers from swabs or from disposable pipetttes used glass slides
uncontaminated waste paper wrappers from swabs or from disposable pipetttes
The total magnification achieved when using a 100X oil immersion lens with 10X binocular eyepieces is ________. 100X 200X 2000X 10X 1000X
1000x
Which of the following objective magnifications is best for observing bacteria? 4x objective. 10x objective. 40x objective. 100X objective.
100X objective
Which of the following objectives should be used with immersion oil? 4x objective 40x objective 100x objective 10x objective
100x objective
What biosafety levels do most introductory microbiology students work with? BSL-2 BSL-1 BSL-3 All of the above levels
BSL-1
Place the following steps of the hand washing procedure in the correct order. 1 Correct: 1 Thoroughly wet hands in running water 4 Correct: 4 Rinse thoroughly in running water. 5 Correct: 5 Dry hands thoroughly and use a towel to turn off the faucet. 2 Correct: 2 Apply soap 3 Correct: 3 Lather for at least 20 seconds by rubbing hands together, paying attention to the back of hands, nails, and between fingers.
Correct: 1 Thoroughly wet hands in running water 4 Correct: 4 Rinse thoroughly in running water. 5 Correct: 5 Dry hands thoroughly and use a towel to turn off the faucet. 2 Correct: 2 Apply soap 3 Correct: 3 Lather for at least 20 seconds by rubbing hands together, paying attention to the back of hands, nails, and between fingers.
Beverages are allowed in the laboratory as long as the containers are covered. True False
False
In science experiments if my hypothesis is not proven as I expected, it's Ok to change it after I see the results obtained. True False
False
The coarse focus knob can be used to adjust the focus when using any of the objective lenses. True False
False
Using a cell phone in lab is permissible since it can be used for timing experiments. T/F
False
You may eat a snack in lab as long as there are no bacterial cultures on your bench and you have thoroughly washed your hands. True False
False
In which circumstance is food and/or beverage allowed in the laboratory? if containers are sealed if containers are covered never if containers are kept out of sight
NEVER
Only lint-free, optically safe tissue should be used to wipe off microscope lenses. True False
True
Watches, bracelets, and rings should be removed prior to washing hands. True False
True
A disinfectant is used on your work surface: before the beginning of laboratory procedures. after all work is complete. after any spill of live microorganisms. all of the above are correct.
all of the above is correct
Infectious laboratory waste is sterilized prior to disposal by autoclaving (steam autoclave) gassing with ethylene oxide soaking in disinfectant incineration
autoclaving (steam autoclave)
Maria wants to know if the amount of sunlight in a garden affects the tomato size?. To answer this question, Maria has 2 plants growing at the same time. Plant # 1 is exposed to more light than plant #2. In this example, the measurement of size of the tomatoes is called __________________? positive control independent variable negative control dependent variable
dependent variable
In the lab, you are using a binocular compound light microscope with 10x oculars. Match the objective used for the total magnification given. 1. Total magnification of 40x. 2. Total magnification of 400x. 3. Total magnification of 100x. 4. Total magnification of 1000x
1 Correct: 1 4x objective 2 Correct: 2 40x objective. 3 Correct: 3 10x objective 4 Correct: 4 100x objective with immersion oil.
Put these lenses in the order they should be used when viewing a specimen with a brightfield microscope. 1 Correct: 1 Low-power lens 2 Correct: 2 High-dry lens 3 Correct: 3 Oil immersion lens
1 Correct: 1 Low-power lens 2 Correct: 2 High-dry lens 3 Correct: 3 Oil immersion lens
How long should hands be lathered while washing? 20 seconds 10 seconds 10 minutes 5 seconds
20 seconds
While using a laboratory photo atlas, a photo is listed as having a total magnification of 400x. Which of the following objectives was used in order to take the photo with a microscope that had 10x oculars? The 20x objective The 400x objective The 4x objective The 40x objective
40x objective
When a hypothesis has been thoroughly supported by long-term study and data, it is considered. a speculation. proved. a theory. a law.
A theory
Maria wants to know if the amount of sunlight in a garden affects the tomato size?. To answer this question, Maria has 2 plants growing at the same time. Plant # 1 is exposed to more light than plant #2. In this example, the amount of light is called __________________? positive control dependent variable independent variable negative control
Independent Variable
Jackie went to the sink at the end of lab to wash her hands. After removing her watch and rings, she turned on the water, wet her hands, added soap and thoroughly rubbed and lathered her hands for 30 seconds, cleaning the backs of her hands, her fingernails, and between her fingers. She then turned off the water, and reached for the paper towels to dry her hands. Identify the correct statement below. Jackie used the correct protocol for washing her hands. Jackie should have applied the soap to dry hands, and then added the water for lathering. Jackie should not have removed her watch and rings prior to washing her hands. Jackie should have used paper towels to turn off the faucet.
Jackie should have used paper towels to turn off the faucet.
Jacob had just finished his lab work and went to the sink to wash his hands. He removed his watch, and added liquid soap to his hands. He then turned on the water and began to rub his hands together, lathering for 20 seconds and cleaning fronts and backs of hands, fingernails, and between his fingers. He then rinsed his hands in running water, dried his hands with paper towels, and used an additional paper towel to turn off the faucet. Which statement is correct? Jacob did everything correctly. Jacob should have wet his hands in running water before applying the soap. Jacob should have washed his hands for at least a minute. Jacob wasted paper towels by using one to turn off the faucet.
Jacob should have wet his hands in running water before applying the soap.
The best source of information regarding the hazards posed by any chemical and specific handling instructions is the lab manual GOOGLE lab partner Material Safety Data Sheet (MSDS)
Material Safety Data Sheet (MSDS)
Where should glass slides, pipettes, and capillary tubes be disposed? sharps container regular trash container biohazard bag recyclable trash container
Sharp container
In order to be called a theory, a scientific idea has to undergo a great deal of testing. True False
True
Negative and positive controls are used during experiments to validate results obtained. True False
True
Once focus is achieved at one magnification, a higher-power objective lens can be rotated into position without fear of striking the slide. True False
True
Which of the following would be most appropriate for disposal of cultures on agar plates? biohazard bag or bin sharps container normal trash receptacle
biohazard bag or bin
Which of the following lenses does NOT affect the magnification of the specimen? high-dry lens condenser lens ocular lens oil immersion lens
condenser lens
Which of the following should be worn at ALL times in the lab? (Check all that apply) lab coat closed-toed shoes face mask protective goggles
lab coat closed toe shoes
