AP BIO EXAM

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Intravenous (IV) solutions administered to patients are normally isotonic. Which of the following is most likely if an IV of distilled water is administered to a patient?

The cells that are exposed to hypotonic solutions will expand as water moves osmotically into the cells from the blood.

Individuals with an inherited autosomal recessive disorder called primary ciliary dyskinesia (PCD) often have severe respiratory problems due to defective cilia. Males with PCD are often sterile because they produce sperm with defective flagella. Which of the following most likely explains the effect of the recessive allele?

The cells do not produce functional motor proteins in flagella and cilia.

The following questions refer to an experiment that is set up to determine the relative volume of O2 consumed by germinating and nongerminating (dry) pea seeds at two different temperatures. The change in volume is detected by using a respirometer over a given period of time. The data are given below. The rate of oxygen consumption in germinating pea seeds at 26ºC is

0.05 mL / min

Based on the information presented, which of the following genetic changes in an individual without diabetes is most likely to result in a disrupted cellular response to insulin signaling similar to that of an individual with type 2 diabetes?

A deletion in the gene encoding the insulin receptor that removes only the cytoplasmic domain of the protein

The figure above shows a model of a ligand precursor being cleaved to produce an active ligand that binds to a specific receptor. Which of the following is most likely to reduce the binding of the active ligand to its receptor?

A mutation in the receptor gene that causes a substitution of a charged amino acid for a nonpolar amino acid in the ligand binding site of the receptor

Gelatin is a protein that is derived from collagen which is found in the bones, skin, and connective tissue of animals. To investigate the ability of various enzymes to digest gelatin, a group of students set up an assay involving camera film. Camera film contains gelatin and appears black when exposed to light but turns clear as the gelatin gets broken down. The students incubated pieces of exposed camera film in test tubes, each containing one of three different enzyme solutions (trypsin, lipase, or amylase) as indicated in Figure 1. The students recorded the time it took for the enzymes to digest the gelatin in each test tube, turning the film from black to clear. Which of the following would be the most appropriate control for this experiment?

A test tube containing a piece of exposed camera film submerged in water

A tissue culture of vertebrate muscle was provided with a constant excess supply of glucose under anaerobic conditions starting at time zero and the amounts of pyruvic acid and ATP produced were measured. The solid line in the graph above represents the pyruvic acid produced in moles per liter per minute. ATP levels were also found to be highest at points A and C, lowest at B and D. A second culture was set up under the same conditions, except that substance X was added, and the results are indicated by the dotted line. Which of the following best accounts for the shape of the solid line between points A and D?

ATP acted as an allosteric inhibitor on one or more of the enzymes.

A tissue culture of vertebrate muscle was provided with a constant excess supply of glucose under anaerobic conditions starting at time zero and the amounts of pyruvic acid and ATP produced were measured. The solid line in the graph above represents the pyruvic acid produced in moles per liter per minute. ATP levels were also found to be highest at points A and C, lowest at B and D. A second culture was set up under the same conditions, except that substance X was added, and the results are indicated by the dotted line. Which of the following best accounts for the shape of the solid line between points A and D?

ATP acted as an allosteric inhibitor on one or more of the enzymes.

the energy required to run the Calvin cycle reactions of photosynthesis comes from which two substances produced during the light-dependent reactions?

ATP and NADPH

Butterflies of the genus Colias live in the Rocky Mountains, where they experience a wide range of temperatures. Different variants of a particular glycolytic enzyme in the flight muscles are optimally active at different temperatures. Within the same population, some individual butterflies fly most effectively at 29°C , while others fly most effectively at 40°C. Still others can be equally active at both temperatures. Which of the following claims is most consistent with the observed butterfly behavior?

Butterflies that express two variants of the enzyme are active over a greater range of temperature.

(A) Glysolysis (B) Krebs cycle (citric acid cycle) (C) Calvin cycle (light-independent reactions of photosynthesis) (D) Light-dependent reactions of photosynthesis (E) Chemiosmosis Process in which carbon from CO2 is incorporated into organic molecules:

C

A student studied the effects of light intensity on oxygen production in green algae. The algae were suspended in water inside a sealed glass jar, and the jar was placed into a constant-temperature, lightproof box containing a light source. A probe was inserted into the jar to record the concentration of oxygen. The probe was connected to a recording device. The setup is shown below. The student decreased the intensity of the light hourly and recorded the corresponding changes in oxygen concentration. The graph below shows the results from the recording device. An increase in the rate of oxygen production by algae would be accompanied by a comparable increase in the rate of production of which of the following substances?

C6H12O6

Aquaporins are channel proteins that facilitate the transport of water across the cell membrane. One group of researchers hypothesizes that without functional aquaporins, no water will be able to enter the cell. A different group proposes an alternative hypothesis, stating that even with nonfunctional aquaporins, a small amount of water will still cross the cell membrane. An experiment is set up in which plant cells with mutated (nonfunctional) aquaporins and plant cells with normally functioning aquaporins are both placed in distilled water. Which of the following data would support the alternative hypothesis?

Cells with mutated aquaporins exhibit moderate turgor pressure and are hypertonic.

Alcohol dehydrogenase (ADH) is an enzyme that aids in the decomposition of ethyl alcohol (C2H5OH) into nontoxic substances. Methyl alcohol acts as a competitive inhibitor of ethyl alcohol by competing for the same active site on ADH. When attached to ADH, methyl alcohol is converted to formaldehyde, which is toxic in the body. Which of the following statements best predicts the effect of increasing the concentration of substrate (ethyl alcohol), while keeping the concentration of the inhibitor (methyl alcohol) constant?

Competitive inhibition will decrease because the proportion of the active sites occupied by substrate will increase.

Alcohol dehydrogenase (ADH) is an enzyme that aids in the decomposition of ethyl alcohol (C2H5OH) into nontoxic substances. Methyl alcohol acts as a competitive inhibitor of ethyl alcohol by competing for the same active site on ADH. When attached to ADH, methyl alcohol is converted to formaldehyde, which is toxic in the body. Which of the following statements best predicts the effect of increasing the concentration of substrate (ethyl alcohol), while keeping the concentration of the inhibitor (methyl alcohol) constant?

Competitive inhibition will decrease because the proportion of the active sites occupied by substrate will increase.

(A) Glysolysis (B) Krebs cycle (citric acid cycle) (C) Calvin cycle (light-independent reactions of photosynthesis) (D) Light-dependent reactions of photosynthesis (E) Chemiosmosis Process in which O2 is released as a by-product of oxidation-reduction reactions

D

The diagram below shows energy changes in a specific chemical reaction with and without the addition of an enzyme to the reaction. Which of the following questions can best be answered by the diagram?

Does the addition of an enzyme reduce the activation energy required for a reaction?

For the following questions: Graphs I-IV depict the effect of pH on the activity of four different hydrolytic enzymes. Graphs representing enzymes sensitive to changes in pH include which of the following?

I, II, and III only

The chemical reaction for photosynthesis is 6 CO2 + 12 H2O + light energy → C6H12O6 + 6 O2 + 6 H2O If the input water is labeled with a radioactive isotope of oxygen, 18O, then the oxygen gas released as the reaction proceeds is also labeled with 18O. Which of the following is the most likely explanation?

During the light reactions of photosynthesis, water is split, removing electrons and protons, and oxygen gas is released.

All eukaryotic cells contain at least one Golgi complex, typically located in the cytoplasm and near the endoplasmic reticulum. Which of the following best describes a process that occurs within the Golgi complex?

Enzymatic modification of newly synthesized integral membrane proteins

Epinephrine is a protein hormone found in many animals. Epinephrine stimulates a signaling pathway that results in the breakdown of glycogen to glucose in the liver cells. Which of the following describes the initial steps in the process whereby epinephrine stimulates glycogen breakdown?

Epinephrine binds to a cell-surface receptor; the activated receptor stimulates production of the second messenger, cAMP.

Which of the following claims is scientifically accurate and consistent with an observation that a decrease in lysosome production within a cell leads to a decline in mitochondrial activity?

Fewer lysosomes will be available to break down macromolecules to provide the necessary nutrients for cellular respiration.

In a mesophyll cell of a leaf, the synthesis of ATP occurs in which of the following? I. Ribosomes II. Mitochondria III. Chloroplasts

II and III only

The enzyme hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, which is an important step in glycolysis. The reaction involves the transfer of a phosphate group from ATP to glucose. Either a glucose molecule or a water molecule can fit in the active site of hexokinase. The presence of a water molecule in hexokinase's active site would result in the hydrolysis of ATP to ADP instead of the conversion of glucose to glucose-6-phosphate. Which of the following statements best helps explain the reaction specificity of hexokinase?

Glucose has the right shape and charge to cause hexokinase to undergo a structural change needed for catalysis, whereas water does not.

Which of the following statements best helps explain the reaction specificity of hexokinase?

Glucose has the right shape and charge to cause hexokinase to undergo a structural change needed for catalysis, whereas water does not.

A researcher claims that the epinephrine signaling pathway controls a catabolic process in muscle cells. Which of the following statements best helps justify the researcher's claim?

Glycogen phosphorylase catalyzes the conversion of glycogen to glucose-1-phosphate.

Phosphofructokinase (PFK) is an enzyme that catalyzes the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate during glycolysis, as represented in Figure 1. PFK can be allosterically inhibited by ATP at high concentrations. Which of the following is the benefit of regulating glycolysis by the concentration of ATP?

Glycolysis proceeds when the intracellular concentration of ATP is low, which provides ATP to drive cellular reactions.

Phosphofructokinase (PFK) is an enzyme that catalyzes the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate during glycolysis, as represented in Figure 1. PFK can be allosterically inhibited by ATP at high concentrations. Which of the following is the benefit of regulating glycolysis by the concentration of ATP?

Glycolysis proceeds when the intracellular concentration of ATP is low, which provides ATP to drive cellular reactions. B

For following group of questions first study the description of the situation or data and then choose the one best answer to each question following it and fill in the corresponding oval on the answer sheet. In the first step of an experiment, rat liver cells were exposed for 5 minutes to amino acids labeled with a radioactive isotope. The cells were then washed to stop any further incorporation of radioactive amino acids. The cells were sampled periodically thereafter, and the radioactivity of a certain protein (protein X) was measured in various cell components, as shown below. The same procedure was repeated with a second protein (protein Y). If the function of Y is protein processing and packaging, the most radioactivity would be found in the

Golgi apparatus

Liver cells manufacture glycoproteins, while adipose cells store fat. Which of the following subcellular structures is likely to be more prominent in liver cells than in adipose cells?

Golgi apparatus

A cell is treated with a drug that prevents the formation of new lysosomes. The cell continues to transcribe the genes that code for the hydrolytic enzymes that are normally found in lysosomes and continues to translate the mRNAs for those proteins on membrane-bound ribosomes. The hydrolytic enzymes are most likely to accumulate in which of the following cellular structures?

Golgi complex

Which of the following best predicts the effect of not having ATP available to supply energy to this process?

H+H+ ions will stop moving through the protein.

The O2 released during photosynthesis comes from

H2O

Which of the following questions is most relevant to understanding the Calvin cycle?

How is ATP used in the formation of 3-carbon carbohydrates?

For the following questions: Graphs I-IV depict the effect of pH on the activity of four different hydrolytic enzymes. Enzymes with their highest activity at an alkaline (basic) pH are represented by which of the following graphs?

II only

Based on the experimental results, which of the following describes the most likely defect in muscle cells of patients with type 2 diabetes?

IRS-1 activation is reduced at high insulin concentrations.

Based on Figure 1, which of the following statements best describes the epinephrine signaling pathway?

In involves enzymes activating other enzymes.

The following questions refer to an experiment that is set up to determine the relative volume of O2 consumed by germinating and nongerminating (dry) pea seeds at two different temperatures. The change in volume is detected by using a respirometer over a given period of time. The data are given below. Which of the following conclusions is best supported by the data?

In pea seeds an increase in temperature results in an increase in oxygen consumption.

Which of the following statements is true regarding the movement of substances across cell membranes?

Ions are unable to move through the phospholipid bilayer because the nonpolar tail regions of the phospholipids are hydrophobic.

Which of the following statements best describes the role of adenylyl cyclase in the epinephrine signaling pathway?

It accelerates the production of a second messenger.

Which of the following statements best describes the role of cyclic AMP in the signal transduction pathway.

It acts as a second messenger that helps relay and amplify the signal within the cell.

Amylase is a protein that catalyzes the conversion of starch to simple sugars. Amylase activity in an aqueous solution can be measured by using iodine as a starch indicator. A solution containing iodine and starch will have a dark-blue color, whereas a solution containing iodine but no starch will have a light-brown color. The color change of an iodine solution from dark blue to light brown can be used to measure the rate at which starch is converted to simple sugars. A student designs an experiment to investigate the effect of environmental pH on amylase function. The design of the experiment is presented in Table 1. Which of the following statements best justifies the inclusion of test tube V as a control in the experiment?

It will show the color change that occurs in the absence of enzyme activity.

The following questions refer to an experiment in which a dialysis-tubing bag is filled with a mixture of 3% starch and 3% glucose and placed in a beaker of distilled water, as shown below. After 3 hours, glucose can be detected in the water outside the dialysis-tubing bag, but starch cannot. If, instead of the bag, a potato slice were placed in the beaker of distilled water, which of the following would be true of the potato slice?

It would gain mass.

The function of which of the following organelles directly requires oxygen?

Mitochondrion

The manner in which several different ions and molecules move through a cell membrane is shown in the diagram above. For each ion or molecule, the relative concentration on each side of the membrane is indicated. Which of the following accurately describes one of the movements taking place?

Na+ transport out of the cell requires ATP hydrolysis.

In chloroplasts, ATP is synthesized from ADP plus inorganic phosphate (Pi) in a reaction catalyzed by ATP synthase molecules that are embedded in the thylakoid membrane. Which of the following statements provides evidence to support the claim that no ATP will be synthesized in the absence of a proton gradient across the thylakoid membrane?

No ATPATP is synthesized when channel proteins that allow the free passage of protons are inserted into the thylakoid membrane.

If ATP breakdown (hydrolysis) is inhibited, which of the following types of movement across cell membranes is also inhibited?

Passage of a solute against its concentration gradient

Which of the following statements best explains the processes of passive and active transport?

Passive transport is the net movement of substances down a concentration gradient that does not require metabolic energy. Active transport is the movement of substances up a concentration gradient that requires energy.

Which of the following best predicts how the structure and function of pepsin will change as it enters the small intestine?

Pepsin will change in shape because of the basic environment of the small intestine; therefore, its enzymatic activity will decrease.

Contains hydrolytic enzymes associated with the intracellular digestion of macromolecules

Peroxisome

A student studied the effects of light intensity on oxygen production in green algae. The algae were suspended in water inside a sealed glass jar, and the jar was placed into a constant-temperature, lightproof box containing a light source. A probe was inserted into the jar to record the concentration of oxygen. The probe was connected to a recording device. The setup is shown below.The student decreased the intensity of the light hourly and recorded the corresponding changes in oxygen concentration. The graph below shows the results from the recording device.Based on the data shown, changes in the light intensity resulted in changes in the rate of which of the following processes?

Photosynthesis

Which of the following experimental designs should the students use as a control for the experiment?

Place a catalase-soaked disk in a beaker of water.

Based on the information presented, which of the following will also occur in response to steroid signaling?

Production of a specific mRNA will increase as a result of the binding of the hormone-receptor complex to the DNA.

Which of the following components of the cell membrane is responsible for active transport?

Protein

Directions: This group of questions consists of five lettered headings followed by a list of phrases or sentences. For each phrase or sentence, select the one heading to which it is most closely related. Each heading may be used once, more than once, or not at all. This group of questions refers to the following groups of biological compounds. (A) Proteins (B) Carbohydrates (C) Nucleic acids (D) Lipids (E) Steroids Synthesized at the ribosome

Proteins

Which of the following can be used to determine the rate of enzyme-catalyzed reactions?

Rate of disappearance of the substrate

A researcher claims that different enzymes exhibit maximal function over different pH ranges. To test the claim, the researcher carries out an experiment that includes three different enzymes: pepsin, salivary amylase, and arginase. The results of the experiment are represented in Figure 1 Which of the following actions will provide the most appropriate negative control for the experiment?

Repeating the experiment with denatured enzymes

A researcher claims that different enzymes exhibit maximal function over different pH ranges. To test the claim, the researcher carries out an experiment that includes three different enzymes: pepsin, salivary amylase, and arginase. The results of the experiment are represented in Figure 1. Which of the following actions will provide the most appropriate negative control for the experiment?

Repeating the experiment with denatured enzymes

Prokaryotic and eukaryotic cells generally have which of the following features in common?

Ribosomes

Cells contain smaller components called organelles that are necessary for a cell's survival. Organelle functions have often been compared to components of larger systems. Which of the following functional differences between the rough and smooth endoplasmic reticulum (ER) is explained by the structural differences between them?

Rough ERER can synthesize and package proteins for export, and smooth ERER cannot.

Cell communication is critical for the function of both unicellular and multicellular eukaryotes. Which of the following is likely true of cell signaling?

Similar cell signaling pathways in diverse eukaryotes are evidence of conserved evolutionary processes.

The salinity of a small inland lake has recently started to increase. Researchers are planning to study the lake over several decades to investigate how freshwater organisms survive significant changes in their natural habitat. Which of the following physiological mechanisms will the researchers most likely observe among the surviving organisms in the lake?

Single-celled organisms will use various mechanisms to counteract the increased flow of water from cells to the environment.

The figure above represents a rough endoplasmic reticulum. Which of the following best describes the role of the structure labeled Y?

Structure Y is the location where proteins are synthesized.

Which of the following organelles modifies and packages for secretion the materials produced by the ribosomes?

The Golgi apparatus

Researchers have proposed a model of the process by which a newly synthesized protein is transported to the plasma membrane and secreted into the extracellular space. The model is represented in Figure 1. Figure 1. A model of the intracellular transport of a newly synthesized secreted protein Based on the model, the newly synthesized protein is transported directly from the endoplasmic reticulum to which of the following?

The Golgi complex

The following questions refer to an experiment in which a dialysis-tubing bag is filled with a mixture of 3% starch and 3% glucose and placed in a beaker of distilled water, as shown below. After 3 hours, glucose can be detected in the water outside the dialysis-tubing bag, but starch cannot. Which of the following best describes the condition expected after 24 hours?

The bag will contain more water than it did in the original condition.

A team of biologists develop a new drug, and one team member hypothesizes that the drug is incapable of freely passing across the plasma membrane and requires the help of membrane proteins to enter cells. Alternatively, another biologist on the team hypothesizes that the drug can diffuse passively across the plasma membrane like O2 and CO2 can. Which of the following, if true about the drug, best supports the alternative hypothesis that the new drug will exhibit simple diffusion across plasma membranes?

The drug is a small nonpolar molecule.

Which of the following best explains how treatment with a drug that stimulates the production of insulin receptors on target cells will affect the insulin signaling pathway in an individual with type 1 diabetes?

The drug will have little or no effect on the signaling pathway because the receptors will not be activated in the absence of insulin.

A researcher proposes a model of an enzyme-catalyzed reaction in which a reactant is converted to a product. The model is based on the idea that the reactant passes through a transition state within the enzyme-substrate complex before the reactant is converted to the product. Which of the following statements best helps explain how the enzyme speeds up the reaction?

The enzyme's active site binds to and stabilizes the transition state, which decreases the activation energy of the reaction.

A student formulated a hypothesis that water-soluble pollutants damage living organisms by increasing the permeability of cellular membranes. To test the hypothesis, the student investigated the effect of isopropanol and acetone on beet root cells. The vacuoles of beet root cells contain large amounts of betacyanin, a water-soluble pigment that is released into the extracellular environment as a result of increased membrane permeability. The student prepared identical samples of beet root tissue and incubated each sample for 15 minutes in the specific solution for that group. At the end of the incubation period, the student measured the absorbance of 460 nm light for each sample. A greater concentration of betacyanin in the solution surrounding the beet root cells results in a greater absorbance of 460 nm light. The results of the experiment are shown in the table above. The illustration above is a model of a typical beet root cell. Based on the experimental results, which of the following best represents the effect of acetone on the permeability of cellular membranes?

The figure shows two models of a cell with a right-facing arrow between the two cells. The cell model on the left shows 4 Betacyanin Molecules inside the Vacuole. The cell model on the right shows 6 Betacyanin Molecules outside the Cell Wall three to the right and three to the left of the cell. The Vacuole membrane and the Plasma membrane are represented with dashed outlines.

Which of the following statements best predicts the effect of a mutation that results in a loss of the glucocorticoid receptor's ligand binding function?

The glucocorticoid receptor will remain associated with the accessory proteins.

Which of the following best describes why the disks rose to the surface faster in the more concentrated hydrogen peroxide solutions?

The higher substrate concentrations in the more concentrated solutions speeded the reaction.

The model below shows the structure of a portion of a plasma membrane in an animal cell. Which statement best explains the orientation of the phospholipid molecules in this model?

The hydrophilic phosphate groups of the phospholipid molecules are attracted to the aqueous internal and external environments.

A researcher designs an experiment to investigate the effect of environmental temperature on the function of an enzyme. For each trial included in the experiment, the researcher will add the enzyme and its substrate to an aqueous buffer solution and then measure the amount of product formed over 20 minutes. Which of the following must remain the same for all trials of this experiment?

The initial concentration of the substrate

A researcher designs an experiment to investigate the effect of environmental temperature on the function of an enzyme. For each trial included in the experiment, the researcher will add the enzyme and its substrate to an aqueous buffer solution and then measure the amount of product formed over 20 minutes. Which of the following must remain the same for all trials of this experiment?

The initial concentration of the substrate

Muscle cells have high ATP demands. Which of the following is a scientific claim about how the structure of the mitochondria in muscle cells should be different than it is in other cells because of the high energy demands of mitochondria?

The inner membrane of the mitochondria in muscle cells should have more folds to increase the surface area, allowing more ATPATP to be synthesized.

Which of the following outcomes will most likely result from the irreversible binding of GDP to the G protein?

The intracellular concentration of glycogen will increase.

Which of the following is an important difference between light-dependent and light-independent reactions of photosynthesis?

The light-dependent reactions produce ATP and NADPH; the light-independent reactions use energy stored in ATP and NADPH.

Which of the following best explains how small molecules move between adjacent cells in a plant shoot?

The molecules pass freely through plasmodesmata, which are cytoplasmic strands connecting two cells.

The following questions refer to an experiment in which a dialysis-tubing bag is filled with a mixture of 3% starch and 3% glucose and placed in a beaker of distilled water, as shown below. After 3 hours, glucose can be detected in the water outside the dialysis-tubing bag, but starch cannot. From the initial conditions and results described, which of the following is a logical conclusion?

The pores of the bag are larger than the glucose molecules but smaller than the starch molecules.

Which of the following is a valid interpretation of the experimental results that explains how individuals with type 2 diabetes differ from individuals without diabetes?

The relatively low levels of glucose uptake in individuals with type 2 diabetes indicate that mobilization of GLUT4 to the cell surface is reduced in muscle cells of those individuals.

Which of the following statements best helps explain the reaction specificity of an enzyme?

The shape and charge of the substrates are compatible with the active site of the enzyme.

In an experiment, cells were isolated from an aquatic plant and suspended in pond water, a sucrose sugar solution, or distilled water. All of the cells were then viewed under a microscope. Compared with the cell in the pond water, the cell in the sugar solution appeared shriveled, and the cell in the distilled water appeared inflated. The results of the experiment are represented in Figure 1. Figure 1. The results of an experiment using aquatic plant cells Which of the following statements best explains the observations represented in Figure 1 ?

There was a net movement of water out of the cell suspended in the sugar solution and a net movement of water into the cell suspended in the distilled water.

Organelles such as mitochondria and the endoplasmic reticulum have membranes that compartmentalize reactions and other metabolic processes. To function properly, the organelles must move substances across their membranes. Which of the following statements describes a feature shared by mitochondria and the endoplasmic reticulum that increases the efficiency of their basic functions?

They have highly folded membranes.

Which of the following statements best justifies the inclusion of test tubes 3 and 7 in the experiment?

They will show whether the isolated cellular contents have enzymatic activity.

Which of the following best describes why ice was used during this experiment?

To retard the breakdown of the catalase

The figure below illustrates a eukaryotic cell. Which of the following best describes how the three structures indicated by the arrows work together?

To synthesize and isolate proteins for secretion or for use in the cell

One approach to treating patients with pancreatic cancer and other cancers in which the Hedgehog protein is detected is to modify the Hedgehog signaling pathway. Which of the following is the most useful approach?

Treating patients with a membrane-soluble compound that can bind to Smo and block its activity

Which of the following scientific questions is most relevant to the model represented in the figure above?

Which molecular substance is actively transported across the plasma membrane?

A tissue culture of vertebrate muscle was provided with a constant excess supply of glucose under anaerobic conditions starting at time zero and the amounts of pyruvic acid and ATP produced were measured. The solid line in the graph above represents the pyruvic acid produced in moles per liter per minute. ATP levels were also found to be highest at points A and C, lowest at B and D. A second culture was set up under the same conditions, except that substance X was added, and the results are indicated by the dotted line. It is most reasonable to hypothesize that, in the breakdown of glucose, substance X is

an inhibitor

It is most reasonable to hypothesize that, in the breakdown of glucose, substance X is

an inhibitor

Simple diffusion and facilitated diffusion are related in that both

are endergonic processes and thus require the hydrolysis of ATP

A researcher claims that increased atmospheric carbon dioxide levels cause increased growth rates in plants. Which of the following statements best supports the researcher's claim?

atmospheric carbon dioxide is the raw material for photosynthesis, which plants rely on for producing sugars and other organic compounds.

The carbon 'that makes up organic molecules in plants is derived directly from

carbon fixed in photosynthesis

The figure above shows a series of microscope images taken over time of ovalbumin production in chick oviduct cells in response to stimulation with estrogen. The ovalbumin protein was detected using a fluorescent dye as shown by the white areas in the time-lapse sequence. The microscope images indicate that ovalbumin

crosses the endoplasmic reticulum membrane as it is translated, then moves to the Golgi apparatus, then to vesicles from which it is secreted

When a substance moves across the plasma membrane along a concentration gradient at a rate faster than would be expected by simple diffusion alone but without the expenditure of metabolic energy, the process is best described as

facilitated diffusion

The enzyme peroxidase is found in many organisms. It catalyzes the breakdown of hydrogen peroxide into water and oxygen gas. The rate of peroxidase activity at different pH values was assessed by students in the lab. The students' results are shown in graph 1. If the experiment is repeated at pH 11, the observed activity level of the enzyme will most likely be

lower than the level at pHpH 9

The organelle that is a major producer of ATP and is found in both heterotrophs and autotrophs is the

mitochondrion

If the potato solution was boiled for 10 minutes and cooled for 10 minutes before being tested, the average time for the disks to float to the surface of the hydrogen peroxide solution would be

more than 30 seconds

For the following questions: Graphs I-IV depict the effect of pH on the activity of four different hydrolytic enzymes. The most likely explanation for the results shown in Graph I is that

pH affects the shape of the active site of the enzyme

Carbohydrate-synthesizing reactions of photosynthesis directly require

products of the light reactions

Precise regulation of specific hormone levels is required for optimal sperm production in mammals, as summarized in the figure above. Anabolic-androgenic steroids (AAS) are synthetic variants of testosterone that are sometimes abused by persons who desire to enhance their athletic performance or alter their physique. Assuming that AAS function in the same way as naturally occurring testosterone, it is most likely that long-term abuse of AAS would

reduce sperm production

For following group of questions first study the description of the situation or data and then choose the one best answer to each question following it and fill in the corresponding oval on the answer sheet. In the first step of an experiment, rat liver cells were exposed for 5 minutes to amino acids labeled with a radioactive isotope. The cells were then washed to stop any further incorporation of radioactive amino acids. The cells were sampled periodically thereafter, and the radioactivity of a certain protein (protein X) was measured in various cell components, as shown below. In graphing the data from this experiment, the sampling time would be shown on the x-axis because

sampling time is the independent variable


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