Basic Microscopy: Microbiology Curriculum
To safely move a microscope a short distance, unplug and secure the cord, turn the head in if it is rotatable, and: a) Pick-up the microscope arm with one hand and carry it with a straight arm alongside your body b) Use two hands to grab each side of the microscope base, and carry it close to your body c) Pick-up the microscope arm with one hand and support the base with your other hand d) Use either a one handed or two handed method to carry the microscope, depending on the distance
C
f you see a blurred image, even after following appropriate processes to focus the microscope, some possible causes could be: (select all that apply) a) Condenser height setting b) Need to set Kohler illumination c) Oil has leaked into the objective d) Slide is upside down
C and D
When cleaning a microscope, it is good practice to do the following: (select all that apply) a) Begin at the oculars and work down to the base b) Clean ocular and objective lenses in a circular motion c) Lower the stage to clean the lens of the condenser diaphragm d) Use 10% bleach as the cleaning solution
Not D
To accommodate the difference in an individual's interpupillary distance, one should adjust the a) Condenser diaphragm b) Objectives c) Oculars d) Stage
ocular
What is the total magnification of an object viewed under the microscope using a 10x ocular and a 20x objective? a) 20x b) 100x c) 120x d) 200x
200x
13. The image contains an organism seen under the microscope, using an ocular micrometer, which has been calibrated to 5 µm= 1 OD. Using the red lines in the picture as a guide, what is the actual measurement of this organism? a) 10-20 µm b) 5-10 µm c) 100-200 µm d) 5 -75 µ
A
Which of the following is not a part of focusing the microscope: a) Begin with the 100x objective b) Use coarse adjustment to bring the object into view c) Use the fine adjustment to obtain a clearer view of the object d) Adjust the light source
A
If your microscope has power, but you see a black field when you look into the oculars (as shown in the following picture below), which of the following should be checked as for potential issues? (select all that apply) a) Bulb condition b) Condenser height setting c) Kohler illumination d) Objectives
All but C
Kohler illumination allows optimal viewing of a specimen by: a) Providing the highest magnification b) Setting the proper light path c) Providing the most light
B
The fine and coarse adjustment knobs are used to perform what action? a) Adjust the intensity of the light b) Focus the microscope c) Move the stage back and forth d) Open and close the condenser diaphragm
B
The most critical step in setting Kohler illumination is the correct adjustment of the: a) Condenser diaphragm b) Field diaphragm c) Light source d) Oculars
Condenser diaphragm
It is acceptable to use your fingers when replacing a bulb in your microscope. True False
False
If you are going to move from 40x to 100x and your microscope is parfocal, which component should you use to focus, once you add oil to the slide and click the 100x objective into place? a) Coarse adjustment knob b) Condenser diaphragm c) Fine adjustment knob d) Stage control knob
Fine adjustment knob
Which of the following does not apply to an ocular micrometer? a) Calibrated using a stage micrometer b) Circular piece of glass with a ruler that is placed inside the ocular c) Standard feature on bright field microscopes d) Used to measure the size of an object
Standard feature on bright field microscopes
Opening or closing the field diaphragm, located on the base of the microscope, controls the width of light entering the condenser diaphragm. True False
True
