Bio 101 w/L Ch 9. Processes & Steps of DNA Replication
How many rounds of DNA Replication were needed to disprove the Conservative method?
1
There are 11 primers for the leading and lagging strand of a particular replication fork. How many Okazaki fragments will there be on one side of the replication bubble?
10 Okazaki fragments
If the DNA sequence is 3' GCCTCC 5', what is the sequence of the primer that begins replication?
5' CGGAGG 3'
What is a function of replication forks?
Allows for bidirectional replication
What kind of organism was used to investigate the method of DNA Replication?
Bacteria
Why do we call DNA an antiparallel molecule?
Because the 3' and 5' ends of each strand run in opposite directions.
In which method of DNA Replication would all parent DNA be conserved in the original molecule?
Conservative
Which is the enzyme that attaches daughter nucleotides to the parent strand during DNA replication?
DNA Polymerase
What is the cellular process in which a new copy of DNA is created?
DNA Replication
Which enzyme is responsible for binding Okazaki fragments together?
DNA ligase
Which enzyme builds the daughter strand alongside the parent strand during cell replication?
DNA polymerase
Which enzyme is responsible for laying down the daughter nucleotides during DNA replication?
DNA polymerase
Which enzyme requires an RNA primer?
DNA polymerase
Why can't DNA polymerase work continuously on both parent strands?
DNA polymerase builds a strand of DNA in the 5' to 3' direction, so it only works on the strand of DNA that runs in the 3' to 5' direction.
In which method of DNA Replication would new DNA consist of alternating parent and daughter DNA?
Dispersive
Where are hydrogen bonds located within the DNA?
In the center of the molecule linking the nitrogen bases of both strands
Why is it necessary that the DNA helicase unwinds the DNA?
It exposes the nitrogenous bases of the nucleic acid chain so that new nucleotides can be added to create the daughter strand.
Who performed the experiment that proved semi-conservative DNA replication was correct?
Matthew Meselson and Franklin Stahl
In the Meselson-Stahl experiment, why does N-15 DNA sink to a lower level than N-14 in a centrifuge tube?
N-15 DNA has a higher density than N-14 DNA.
How is the N-15 isotope different from the N-14 atom?
N-15 has one extra neutron.
What are the small components (monomers) that make up the large DNA polymer?
Nucleotides
Which is the enzyme that builds the RNA primer?
RNA Primase
Which is the strand that replicates just as quickly as the replication fork opens up?
The leading strand
What happens to the RNA primer once DNA replication is begun by DNA polymerase?
The primer is removed.
Which word part is used to designate that a molecule is an enzyme?
The suffix '-ase'
How can a mutation in one copy of a DNA sequence be created?
The wrong base pair is added during synthesis
The replication of DNA is a complex process; all of the following statements are correct, EXCEPT
There is one replication fork in one replication bubble.
Which of the following BEST explains the role of DNA polymerase?
To assemble daughter nucleotides on the parent strand.
What is the purpose of the RNA primer?
To initiate the process of DNA replication.
Using the given information, determine the correct order of the following events during the replication of the lagging strand. a. The DNA double helix unwinds. b. The Okazaki fragments are joined. c. The RNA primase builds an RNA primer on the parent strand. d. Nucleotides are added and matched to the parent strand.
a, c, d, b
What might be the result of a decrease in the number of replication bubbles?
an increase in replication time
The location where the process of DNA replication begins is called the _____.
origin of replication
Which is a feature of the semi-conservative model of DNA replication?
replicated DNA molecules have a parent strand and a daughter strand
When DNA helicase separates the two parental strands, it creates a Y-shape called a _____.
replication fork
Because hydrogen bonds are weak bonds, they create a perfect site for _____.
separating nucleic acid strands
Which of the parent DNA strands is replicated discontinuously?
the lagging strand
The Dispersive method of DNA Replication was disproved when the scientists observed...
two separate bands of DNA of different densities.
