Bio lab practical 2?

A microtubule structure in animal centrosomes.

centriole

Where sister chromatids are attached.

centromere

Microtubule organizing center.

centrosome

A groove at the cell surface located between dividing animal cells.

cleavage furrow

A cell with two nuclei.

dikaryon

Chromosomes that have the same set of genes, one from the mother and one from the father.

homologous pair

A protein structure attached to the centromere.

kinetochores

Fusion of an egg with more than one sperm.

polyspermy

A membrane structure located between dividing plant cells.

. cell plate

If a cell in G1 has 24 chromosomes, then at the end of meiosis I there will be _________ chromosomes in each daughter cell, and __________ sister chromatids in each daughter cell.

12, 24

located within the gel; binds DNA and fluoresces

Nucleic acid stain

If a "unit" is defined as the amount of enzyme needed to digest 1μg of DNA in 1 hour at 37°C, how many units would it take to digest 10 ug of DNA in 20 min?

30

Which of the following is a palindromic restriction site?

5' CCATGG 3'

Counting up spores and asci from Sordaria, you find there are 24 asci that have a 4:4 pattern of spores and 36 asci that have a pattern of 2:4:2 or 2:2:2:2. The percent recombination is ________ and the map units between centromere and pigment gene is _________.

60, 30

If a cell in G1 has 32 chromosomes, then in G2 there will be _________ sister chromatids, and __________ chromosomes after mitosis.

64, 32

In this lab you will use Sfi l, which is:

A Restriction enzyme

maintains pH and ionic conditions required for each restriction enzyme in order to maximize its activity

Buffer

Reciprocal exchange of genetic material between non-sister chromatids.

Crossing-over

In the process of DNA purification, what is the purpose of adding detergent?

Destroys the cell plasma membrane and nuclear envelope

During ___________ the chromosomes of the cell are duplicated.

Interphase

mixed with DNA to make it more dense and visible

Loading dye

For each cell that enters this process one daughter cell is produced. (Check all that apply.)

Oogenesis

small circular DNA molecule

Plasmid

enzyme that recognizes a specific DNA nucleotide sequence and cuts the DNA at or near this site

Restriction endonuclease

When nuclear material is duplicated.

S phase

Which of the following is a diploid male cell?

Spermatogonium

overhanging single-stranded DNA

Sticky ends