Bio-safety Course

Lakukan tugas rumah & ujian kamu dengan baik sekarang menggunakan Quizwiz!

Airflow rates into the front access opening of the biosafety cabinet are in the following range:

100 - 110 feet per minute

Animal handlers should be screened and medically cleared by the Employee Health Office or designated medical health official before working with animals.

True

NIH funded institutions conducting research with recombinant and synthetic nucleic acids must have at least this many community members on their IBC (with the requirement that community members must be from the community and not affiliated with the institution):

2

How many Biosafety Levels have been created to outline recommended biocontainment practices and laboratory design considerations?

4

How many different Risk Groups have been establishes for categorizing Pathogen severity and risk assessment starting point?

4

What is the recommended NIOSH height (in inches) for the top opening of wall mounted sharps containers?

52 to 56 inches

Which location is not appropriate for hand washing after working with human material in the laboratory?

A hand washing sink in the break room

Which of the following is not considered an action that would interfere with the airflow patterns at the front opening of the biosafety cabinet?

A researcher discarding waste within the biosafety cabinet to prevent the movement of their arms in and out of the front opening

The annual IBC report to the NIH shall include:

A roster of all Institutional Biosafety Committee members clearly indicating the Chair, contact person, Biological Safety Officer (if applicable), plant expert (if applicable), animal expert (if applicable), human gene therapy expertise or ad hoc consultant (if applicable); and biographical sketches of all Institutional Biosafety Committee members (including community members).

Which response best identifies why a surgical mask should not be used as a respiratory to protect against the inhalation of biohazards or bioaerosols?

A surgical mask may not be impervious to liquids

What does each employer that has employees with occupational exposure to human blood or OPIM need to have?

A written Exposure Control Plan that identifies who is exposed and outlines protective measures.

The following elements are associated with the pathogen in performing a risk assessment:

All of the above

The following risks should be considered in formulating safety protocols for experiments involving research animals.

All of the above

Which of the following is the best response applicable to personnel considerations as part of the risk assessment process?

All of the above

Which of the following is not required by the employer following an exposure incident to human blood or OPIM?

An annual medical physical

A health history evaluation or questionnaire is most likely completed in the following circumstance:

As a screening mechanism before workers initiate laboratory work to identify critical risk factors for the individual

This Biosafety Level requires at least two-door separation between the area where biohazards are used and the personnel not associated with the research, requires inward inflow into the lab and must have a visual mechanism to verify that air is flowing in the appropriate direction.

BSL3

Which of the following is not an important medical surveillance item to review with staff handling biohazards?

Biocontainment level

Which pathogen poses the greatest risk to a researcher handling non-human primates if they are exposed and infected with the infectious agent:

Cercopithecine Herpes virus-1 (B-virus)

Which of the following is not an exposure route for a biohazard in the laboratory setting?

Contact of biohazard on hair

Which of the following is a recommendation to follow when using a biosafety cabinet to promote safe operation?

Do not block the front or rear grilles while the cabinet is in operation

Which of the following is not part of periodic health screening for workers handling biohazards?

Document that researchers have an active life insurance policy

What response best describes the permeability of exam gloves used in laboratories?

Exam gloves are not 100 percent leak proof, as leak rates associated with manufacturing range from one to three percent.

Experiments using Risk Group 2, 3, 4 or restricted agents as host vector systems fall under which category of experiments? In other words, recombinant or synthetic nucleic acid molecules into a Risk Group 2 viral vector such as adenovirus which is used with tissue culture.

Experiments that require IBC approval before initiation (Section III-D)

Experiments involving the administration of a wild-type (NOT recombinant) animal virus to transgenic rodents created by this laboratory fall under which category of experiments?

Experiments that require IBC approval simultaneous with initiation (Section III-E)

Experiments involving the cloning of toxin molecules with LD50 < 100 ng/kg fall under which category of experiments?

Experiments that require NIH OSP and IBC approval before initiation (Section III-B)

Which is the best step to follow in the immediate aftermath of an occupational exposure incident to the eyes, nose, or mouth involving human blood or OPIM?

Immediately wash the exposed area with soap and water (or use an eye wash for 15 minutes). Then seek medical follow-up at the designated emergency healthcare location.

A clean bench is not recommended for containment of biohazards because:

It directs clean air across the work surface, then pushes air from inside the bench to outside and directly at the operator

Which of the following best describes the universal biohazard label?

Labels are fluorescent orange or orange-red with lettering and symbols in a contrasting color.

Which of the following is appropriate for the decontamination of liquid biomedical waste?

Liquid biohazardous waste can be decontaminated with household bleach diluted to a final concentration of at least 10 percent against the volume of waste that will be treated for at least 30 minutes.

An effective mechanism to ensure that a risk assessment is conducted is:

Mandatory Biohazard Protocol Registration

Which of the following best describes the requirements associated with the use of a tight fitting respirator?

Medical clearance, respirator fit testing, and respiratory protection training

As allergens are very small particles and may remain suspended in air if aerosols are generated, every effort should be applied to contain them as close to their source point as possible by using filtered cages, biological safety cabinets, and other negative pressure filtered devices

True

Consideration of the proposed research protocol procedures is one of the steps in risk assessment.

True

A formal risk assessment is NOT required if researchers will wear personal protective equipment consisting of gloves, a lab coat, and safety glasses.

False

Animal allergens can only infect workers through inhalation, or breathing in small airborne particles

False

As there are no published listings of risk groups for human pathogens anywhere in the world, Principal Investigators must start by developing a Risk Group ranking for the pathogen they propose using in research.

False

For biohazard experiments, hands-on training is not required provided researchers have successfully completed their formal biosafety training classes.

False

Given the wide range of personal protective equipment, work practice controls and containment equipment, Principal Investigators or Institution's never have to exclude or prohibit anyone from an experiment.

False

Procedures involving sharps, such as needles, scalpels, or glass Pasteur pipettes DO NOT represent risk if the manipulations with biohazards occur within a biological safety cabinet.

False

Risk assessment and risk management determinations for biohazard research involving animals do not require additional expertise beyond the biosafety officer.

False

The use of open top cages for animals that are documented free of pathogens does not represent a risk to animal handlers.

False

In the U.S., how long are employers required to maintain their employees' medical records?

For the duration of employment plus 30 years

Which of the following statements is most accurate?

HBV vaccine is recommended for unvaccinated adults who are at risk for HBV infection.

Employees with occupational exposure to human blood, body fluids, and OPIM need training. Which of the following statements is correct regarding training for employees?

OSHA Bloodborne Pathogens Standard training is required for occupationally exposed employees prior to the initiation of work involving exposure and annually within 365 days of the previous training.

Which answer best describes PPE on the hierarchy of controls to protect workers?

PPE is considered the last line of defense to protect researchers on the hierarchy of controls.

Which of the following statements regarding routes of exposure to HBV is correct?

Percutaneous (through the skin) and mucous membranes (for example, splashes to the eyes, nose, and mouth) are primary routes of exposure to HBV.

Which item is not considered PPE?

Personal identification badge

The types of incidents that must be reported to the NIH OSP by NIH funded institutions under the NIH Guidelines include which of the following?

Personal injury involving rDNA materials

Which of the following are areas the IBC should review in research coming to the committee?

Potential risk to the environment and public health with an assessment of the containment levels, facilities, procedures, practices, training and expertise of personnel

Salmonella and Shigella are examples of biohazards that have which Risk Group as their starting point in the risk assessment process?

Risk Group 2

Salmonella and shigella are examples of which Risk Group agents?

Risk Group 2

Mycobacterium tuberculosis, a human pathogen with an inhalation route of exposure, a low infectious dose and is capable of causing severe disease, including death, is representative of which Risk Group?

Risk Group 3

Ebola virus is an example of a pathogen associated with which Risk Group?

Risk Group 4

In Risk Group Comparison Table, Gwladys Caspars' quick guide statement of "don't touch it refers to biohazards in which Risk Group?

Risk group 2

Which category of the NIH Guidelines describes experiments which are EXEMPT from the Guidelines?

Section III-F

Which of the following is not a recommended practice for sharps containers?

Sharps containers do not have to be emptied until they are completely full.

Which of the following is a true statement regarding engineering controls?

Sharps containers, biohazard bags, tongs, gauze pads, bench top shields, sealed tubes, and centrifuge safety buckets are all examples of other engineering controls that are available for worker protection.

What is the best recommendation to provide staff who have breaks in their skin, but must work with biohazards?

Staff should not work with biohazards when they have breaks in their skin.

Which of the following statements is accurate regarding U.S. biohazard regulations?

The OSHA General Duty Clause (Section 5A1) can be applied to biohazards in the research laboratory.

What is the central focus of the OSHA Bloodborne Pathogens Standard?

The standard discusses a framework to minimize exposure to bloodborne pathogens such as HIV, HBV, and HCV viruses that could be present in human blood, tissues, and body fluids.

What is the recommended schedule for the administration of the HBV vaccination?

Three shots - one each on Day 0, Day 30, and Day 180

Which of the following exposure routes may result in transmission of bloodborne pathogens?

Through skin or facial mucous membranes of eyes, nose, or mouth

When considering risk from biohazard experiments involving research animals, shedding of the biohazard is an important factor.

True

Which of the following statements is correct regarding the responsibilities of the IBC?

Upon request, the institution and IBC shall make available to the public all Institutional Biosafety Committee meeting minutes

Which of the following procedures are associated with the generation of biohazardous aerosols?

Vortexing

Which response provides the best description for proper hand washing?

Wash hands for 30 seconds in total starting by rinsing first, then washing and lathering with soap, followed by a final rinse to remove soap and residue from hands.

Which of the following is the best example of an appropriate response to a spill of human material involving broken glass or other sharps?

When responding to spills of human blood or OPIM that also involve broken glass or other sharps, a set of tongs or another device must be used to collect contaminated sharps. This eliminates the potential of exposure from direct collection by hand.

Which of the following is true regarding an exposure to human blood or OPIM for an individual who has not received the hepatitis B virus (HBV) vaccination?

Workers who have not received the HBV vaccination can be provided with hepatitis B immune globulin and start the HBV vaccine series in the immediate aftermath of an exposure incident to human materials.

Which occupational health disease is the most prevalent among animal handlers and may impact up to 30% of individuals who work with animals.

animal allergy

If an institution conducts research involving recombinant or synthetic nucleic acid molecules in rodents, the IBC is required to have members or ad hoc consultants with expertise in:

animal containment principles when experiments require Institutional Biosafety Committee approval prior to initiating the research, and implementation of Appendix M

How long do employers need to keep the records for OSHA Bloodborne Pathogens Standard training?

for at least 3 years


Set pelajaran terkait

CompTIA(220 - 1001) - Networking (Test Prep)

View Set

WEEK 2 [BSN 340] "FEEDING, EATING AND PERSONALITY DISORDERS"

View Set

Ch 9 Obversion, Conversion, and Contraposition

View Set

ATI Health Assess 2.0: Rectum and Genitourinary

View Set

Econ Final Study Guide Chapter 2

View Set

Ch 44 Drugs Acting on the Renin-Angiotensin-Aldosterone System

View Set

AP Biology Chapter 17 Study Guide

View Set