Biology 2051 Lab Chapter 24

Enzyme that hydrolyzes Gelatin

Gelatinase

Caseinase product

peptides

Gelatinase product

peptides

Polypeptides are comprised of _________ subunits linked by _________ bonds.

amino acids peptide

The bonds connecting amino acids in proteins are _______ ________ ________.

Covalent Peptide Bonds

Caseinase positive organisms

Bacillus subtilis

Gelatinase positive organism

Bacillus subtilis

A protein that is colloidally suspended in milk giving it its white opaque appearence

Casein

________ is the main protein of milk which gives milk its opacity. One would look for _____________ on the milk agar plate if this protein is hydrolyzed.

Casein clearing

Enzyme that hydrolyzes Casein

Caseinase

Why is gelatin not suitable as a solidifying agent in bacteriological media?

It liquifies if above 28C and many microorganisms contain gelatinase, which breaks down the gelatin

What must one do to observe gelatinase production with the plate method?

add an acid like HCl

Enzyme that hydrolyzes proteins into peptides (short chains of amino acids).

Proteases

Proteins are made of ___________.

amino acids

Gelatinase substrate

gelatin

Gelatinase media

gelatin agar

_________ causes the break down of the peptide bonds between amino acids of the protein gelatin.

gelatinase

Gelatinase reagent

hydrochloric acid

Caseinase substrate

milk

Caseinase media

milk agar

Caseinase reagent

none

Caseinase positive results

zone of hydrolysis

Gelatinase positive results

zone of hydrolysis