ch 12

The crime scene sample contains DNA fragments from both the victim and Suspect 1.

Why is Suspect 1 considered more likely to have committed the crime than Suspect 2?

In gel electrophoresis DNA molecules go from _____ to _______ ends of the gel

negative --> positive

How does the company raising these fish claim to prevent the genetically modified fish from breeding with wild fish?

the genetically modified fish are sterile

The state of human gene therapy today is that

there have been a small number of successes, including with the disease SCID.

A DNA fragment with the end sequence TGGCA will bind with another DNA fragment withthe sticky end sequence _______.

ACCGT

Which enzyme is used to bind DNA fragments together?

DNA ligase

What is the best definition for recombinant DNA?

DNA that includes nucleotides from two different sources

Which of the following is true

Gene therapy is generally more accepted than germline editing.

Which of the following is an example of editing the human germline?

Making a change in someone's DNA that will be passed on

production of a human protein that does not function normally

Recombinant DNA technology produces transgenic bacteria with a human gene that codes for a human protein. What problem could be associated with this process?

human gene therapy

The diagram below summarizes

-ACGT

The sticky end of the DNA restriction fragment shown here will pair with a DNA restriction fragment with the sticky end _____. Sticky end of DNA in the figure has a nucleotide sequence TGCA. The nucleotide sequence is given from the edge of the sticky end to the rest of DNA molecule; that is, from the left to the right.

What concerns do some consumer groups have about genetically modified fish?

They want more studies on the health effects that genetically modified fish may have on people who eat it. They want to prevent genetically modified fish from breeding with wild fish. They want food from genetically modified fish to be clearly labeled as genetically modified. (all of the above)

B

This is a DNA fingerprint exhibiting samples from a victim, two suspects, and the crime scene. Which of these DNA fragments is common to both the victim and Suspect 1?

C

This is a DNA fingerprint exhibiting samples from a victim, two suspects, and the crime scene. Which of these DNA fragments is common to both the victim and Suspect 2?

E (the shortest is farthest away)

WHich is the shortest

When is PCR particularly applicable?

When there are small quantities of DNA to analyze

DNA fingerprinting is used to solve paternity cases; can it also be used to study evolutionary connections?

Yes. Specific genetic markers can reveal evolutionary relationships between populations that share those markers.

The modified salmon were created by _____.

adding genetic material from a Pacific salmon and an eel-like fish

What is the definition of a "transgenic organism"?

an organism containing a gene from another species

The success of Crispr-Cas9 lies with which of the following?

destroying some of the persons DNA

You are a molecular biologist working on a genetic treatment for hypercholesterolemia using Crispr-Cas9. Who will you need permission from to have your treatment approved for use in the U.S.?

food and drug administration

For the first time, the U.S. Food and Drug Administration is considering whether to allow the sale of _____.

food from a genetically altered animal

The fish in the video have been genetically engineered to _____.

grow faster

In a PCR reaction, the strands of DNA are first separated by ___.

heat

The world's first genetically engineered pharmaceutical product was ______.

humulin

Gel electrophoresis separates DNA fragments on the basis of differences in their ____________.

length

According to the producers of the genetically modified salmon, the meat _____

looks and tastes the same as unmodified salmon

In human gene therapy ________

normal versions of genes are transferred to patients who carry a mutated allele.

What can act as a vector to introduce new genes into a cell?

plasmids

You are the CEO of a start-up that has a goal of improving the first technique used to manipulate genetic material. Which of the following is your company working on?

recombinant DNA

what is the correct sequence of events that occur in a PCR reaction?

separation of DNA strands; addition of primers; use of DNA polymerase to produce second strand of DNA

The unpaired nucleotides produced by the action of restriction enzymes are referred to as _____.

sticky ends (They are called sticky ends since they will "stick" to a complementary single-stranded sequence.)

DNA polymerase is a heat-sensitive enzyme. What is one thing that would need to be considered concerning the activity of this enzyme in PCR when the temperature is heated during each cycle to separate the DNA strands?

that the DNA polymerase could be denatured