CH 2

What is the total magnification of a specimen that is being viewed with a standard ocular lens and a 40⨯ objective lens?

400x

2. You place a specimen under the microscope and notice that parts of the specimen begin to emit light immediately. These materials can be described as _____________. a. fluorescent b. phosphorescent c. transparent d. opaque

A

. What is one difference between specimen preparation for a transmission electron microscope (TEM) and preparation for a scanning electron microscope (SEM)? a. Only the TEM specimen requires sputter coating. b. Only the SEM specimen requires sputter- coating. c. Only the TEM specimen must be dehydrated. d. Only the SEM specimen must be dehydrated.

B

4. Who is the probable inventor of the compound microscope? a. Girolamo Fracastoro b. Zaccharias Janssen c. Antonie van Leeuwenhoek d. Robert Hooke

B

7. Which type of microscope would be the best choice for viewing very small surface structures of a cell? a. a transmission electron microscope b. a scanning electron microscope c. a brightfield microscope d. a darkfield microscope e. a phase-contrast microscope

B

1. Which of the following has the highest energy? a. light with a long wavelength b. light with an intermediate wavelength c. light with a short wavelength d. It is impossible to tell from the information given.

C

5. Which would be the best choice for viewing internal structures of a living protist such as a Paramecium? a. a brightfield microscope with a stain b. a brightfield microscope without a stain c. a darkfield microscope d. a transmission electron microscope

C

9. What type of microscope uses a cone of light so that light only hits the specimen indirectly, producing a darker image on a brighter background? a. a transmission electron microscope b. a scanning electron microscope c. a brightfield microscope d. a darkfield microscope e. a phase-contrast microscope

D

What mordant is used in Gram staining? a. crystal violet b. safranin c. acid-alcohol d. iodine

D

Which type of microscope is especially useful for viewing thick structures such as biofilms? a. a transmission electron microscope b. a scanning electron microscopes c. a phase-contrast microscope d. a confocal scanning laser microscope e. an atomic force microscope

D

Who was the first to describe "cells" in dead cork tissue? a. Hans Janssen b. Zaccharias Janssen c. Antonie van Leeuwenhoek d. Robert Hook

D

8. What type of microscope uses an annular stop? a. a transmission electron microscope b. a scanning electron microscope c. a brightfield microscope d. a darkfield microscope e. a phase-contrast microscope

E

The _______ is used to differentiate bacterial cells based on the components of their cell walls.

Gram stain

When you see light bend as it moves from air into water, you are observing _________.

Refraction

Ziehl-Neelsen staining, a type of _______ staining, is diagnostic for Mycobacterium tuberculosis.

acid-fast

In a(n) _______ microscope, a probe located just above the specimen moves up and down in response to forces between the atoms and the tip of the probe.

atomic force microscope

A microscope that uses multiple lenses is called a _________ microscope.

compound

Chromophores that absorb and then emit light are called __________.

fluorochromes