CH 20 AP BIO

In recombinant methods the term "vector" refers to

A plasmid or other agent used to transfer DNA into a living cell

What two enzymes are needed to produce recombinant DNA?

A restriction enzyme and a ligase

A scientist wishing to create an organism capable of breaking down several kinds of toxic waste combines genes from several species of bacteria to create a single superbacterium. Which of the following would be needed to do this?

All of the above

Separating DNA fragments by gel electrophoresis is useful for which of the following?

All of the above

To create DNA with long-term stability, it is necessary to have which of the following in the test tube?

DNA ligase

Bacteria use restriction enzymes to

Destroy foreign DNA

A nucleic acid probe is used to

Identify genes that have been inserted into bacterial plasmids or separated by electrophoresis

In genetic engineering, "sticky end" refers to

Short bits of single-stranded DNA left at the end of DNA molecules but by restriction enzymes

DNA fingerprints are used to determine whether Sam could be the father of Becky's baby. Sam is not the father if ___ genetic fingerprint shows some bands not present in ___ genetic fingerprint.

The baby's; Sam's or Becky's

In polymerase chain reaction (PCR) technique, a heating phase and cooling phase alternate. An original sample of DNA would have to pass through how many total rounds of heating and cooling before a sample is increased eight times in quantity?

Three