chapter 19 pt2
regarding gene therapy strategies in cancer: during the tumor suppressor gene activation, the transferring of tumor suppressor genes into cancer cells can cause what 2 things
- cell cycle inhibition - apoptosis being promoted
what are the 5 types of gene manipulations
1. gene replacement 2. gene knockdown 3. genome editing 4. genome modification 5. noncoding RNA modulation
what are the two types of vectors used for gene delivery into cells
1. non viral vectors 2. viral vectors
regarding gene therapy strategies in cancer: angiogenesis is an essential process for what 3 things
1. tumor progression 2. growth 3. metastasis
regarding gene therapy strategies in cancer: during anti-angiogenesis silence angiogenic inducers (VEGF, angiopoietin) are used to boost endogenous ....
angiogenic inhibitors (angiostatin, endostatin and IL-12)
regarding gene therapy strategies in cancer: during immunotherapy, what is done to the host immune system to eliminate target cancer cells
augmentation
regarding gene therapy strategies in cancer: during the tumor suppressor gene activation the cell cycle is inhibited or apoptosis is promoted by transferring what into what
by transferring tumor suppressor genes into cancer cells
in cancer, immune cells seem to be tolerant to cancer cells like their normal body cells, they cannot initiate an effective immune response against _ as they do for bacteria and viruses
for tumor cells
the advantages of using viral vectors for gene delivery into cells are that viruses may be adapted for ... (2)
may be adapted for long lasting gene therapy or transient transgene expression
which vector type has advantages such as biosafety, low immunotoxicity and reduced pathogenicity
non viral vectors
regarding gene therapy strategies in cancer: oncogenes are often over expressed in tumors, contributing to the conversion of ...
of normal cells to cancer cells
regarding gene therapy strategies in cancer: Antisense Oligonucleotides (ASOs), siRNA and miRNA molecules are used for the inhibition of what
of oncogene protein synthesis
regarding gene therapy strategies in cancer: oncogenes are often expressed how in tumors
overexpressed
what is the most important and most studied tumor suppressor gene
p53
regarding the types of gene manipulations: what is gene replacement
replacing the faulty or non working gene w a healthy copy
what is the most common viral vector used in clinical trials to treat cancer
retrovirus
regarding gene therapy strategies in cancer: during anti-angiogenesis what are used to boost endogenous angiogenic inhibitors like angiostatin, endostatin and IL-12
silence angiogenic inducers (VEGF, angiopoietin)
regarding the types of gene manipulations: what is genome editing
snipping out the faulty part of the gene and changing the cancers DNA
regarding gene therapy strategies in cancer: what is angiogenesis
the formation of new blood vessles
in cancer, immune cells seem to be tolerant to what type of cells
to cancer cells like their normal body cells
do viral or non viral vectors have an efficient gene transfer into cells
viral
