Chapter 8: Mutations - Ames Test

Ames Test

A procedure using bacteria to identify potential carcinogens

After the Ames Test has been completed, one must compare the numbers of mutants to a ______ (positive or negative) control that contains _________ + __________ extract.

After the Ames Test has been completed, one must compare the numbers of mutants to a negative control that contains water + rat liver extract.

What is Ames Test used for?

Ames Test is used to screen for chemicals that may be mutagens

What is the third step in the Ames Test?

EXPOSE bacteria to the mutagen-rat liver mix

T or F: Only chemicals cause mutagenic exchange, or mutants, in the Ames Test.

False - Life causes mutagenic exchange, or mutants, in the Ames Test.

Why are mutagens mixed with rat liver extract?

In order to simulate body chemistry

What causes a significant number of mutant colonies during the Ames Test?

Mutagens cause a significant number of mutant colonies

What survives after all of the steps of the Ames Test have been completed?

Only mutants survive

What is a serious real problem of the Ames Test?

The Ames Test is way too sensitive

What is the first step in the Ames Test?

The SELECTION of the suspected mutagen

What is the second step in the Ames Test?

The selected mutagen is MIXED with rat liver extract

T or F: A way to find out if mutants survived the Ames Test procedure steps is by counting the number of mutants that survived.

True