CITI initial biosafety training
Which dilution of hydrogen peroxide would not be considered a broad spectrum and sporicidal disinfectant?
0.5 percent
The best response for individuals who handle biohazards who have an existing cut or open wound on their hands is?
Not work with biohazards until the cut is healed or cleared by a healthcare professional to work with the use of waterproof bandages and double gloves.
Which of the following statements is accurate regarding laboratory-acquired infections?
Not all laboratory-acquired infections have an identified route of exposure.
Which answer best describes PPE on the hierarchy of controls to protect workers?
PPE is considered the last line of defense to protect researchers on the hierarchy of controls.
Which statement regarding an autoclave and BSL3 laboratories is the most accurate?
Pass-through autoclaves are recommended for BSL3 laboratories
Which of the following is true about researchers' pets in general?
Pets or animals that are not associated with the research are not permitted in the laboratory
Which of the following is considered a high level disinfectant that would inactivate biological spores?
10 percent household bleach in water solution
What is the recommended minimum temperature setting for an autoclave to inactivate biohazards, including spores, if it is operated for the appropriate contact time?
121 ºC (250 ºF)
The length of time recommended in the emergency response module to flush your eyes in an eye wash following an ocular exposure to a biohazard is?
15 minutes
How long should researchers wash their hands with soap and water after handling biohazards, removing PPE and clothing, and before leaving the laboratory?
25 to 30 seconds
When centrifuging biohazards, what is the recommended "wait time" before opening the centrifuge to allow aerosols to settle in the event of a spill or leak from a primary tube?
3 to 5 minutes
How many Biosafety Levels have been created to outline recommended biocontainment practices and laboratory design considerations?
4 (There are four Biosafety Levels for the safe handling and containment of biohazards: Biosafety Level 1, 2, 3 and 4. Biosafety Level 1 is for those microorganisms and other materials that require the least amount of containment. BSL4 is reserved for those biohazards that require the most stringent biocontainment.)
Based on the information in this module, a biohazard spill that involves release from a centrifuge to the open lab is considered as?
A high risk biohazard release with significant potential for aerosol generation and requires immediate evacuation from the lab.
On the quick summary table of biocontainment or Biosafety Levels (BSL's), what is the lowest BSL that recommends the use of a biological safety cabinet to contain aerosols based on risk assessment?
BSL2 (BSL2 is the first biosafety level or biocontainment level that recommends the use of a biosafety cabinet to contain aerosols based on risk assessment.)
The installation of hands-free sinks is first required for which biosafety level?
BSL3
What is the lowest BSL that requires all manipulations of biohazards to take place within biological safety cabinets or other primary containment enclosures?
BSL3 (What is the lowest BSL that requires all manipulations of biohazards to take place within biological safety cabinets or other primary containment enclosures?)
Which laboratory must have an effluent decontamination system to inactivate liquid wastes?
BSL4
This BSL requires the use of a supply line respirator or all work within a sealed totally enclosed glove box.
BSL4 (BSL4 is the biocontainment level that requires either a encapsulated suit with a supply airline respirator or all work with sealed Class III Glove Boxes.)
Which of the following is not an important medical surveillance item to review with staff handling biohazards?
Biocontainment level
Which of the following is not an exposure route for a biohazard in the laboratory setting?
Contact of biohazard on hair
In a research or cell culture lab where no animals are in use, what is the best recommendation regarding sharps based on safe sharps work practices?
Eliminate all sharps and use plastic alternatives wherever feasible
Which of the following is a recommendation to follow when using a biosafety cabinet to promote safe operation?
Do not block the front or rear grilles while the cabinet is in operation
Which of the following is not part of periodic health screening for workers handling biohazards?
Document that researchers have an active life insurance policy
Which of the following statements accurately reflects the range of engineering controls used for protection against biohazards?
Engineering controls can be sophisticated devices (such as biosafety cabinets) and very simple devices (such as bench shields or biohazard waste receptacles).
Per the information provided in this module, the minimum personal protective equipment required to clean and disinfect a spill of a Risk Group 2 biohazard that requires Biosafety Level 2 containment (after allowing at least 30 minutes to allow aerosols to settle) consists of?
Gloves, a lab coat and full face protection
The number of laboratory-acquired infections is best described as?
Greater than 5,000 laboratory-acquired infections have been documented.
According to this module, which statement regarding exposures in the laboratory to biohazards is accurate?
Have led to secondary infections of those who don't work inside the laboratory.
Which of the following statements regarding laboratory-acquired infections is true?
In one major study of laboratory-acquired infections, 80 percent of the laboratory-acquired infections had an unknown route of exposure.
A clean bench is not recommended for containment of biohazards because:
It directs clean air across the work surface, then pushes air from inside the bench to outside and directly at the operator
When centrifuging biohazards, it is recommended that the safety buckets and sealed rotors be:
Loaded and unloaded within the biosafety cabinet
An effective mechanism to ensure that a risk assessment is conducted is:
Mandatory Biohazard Protocol Registration
Which of the following best describes the requirements associated with the use of a tight fitting respirator?
Medical clearance, respirator fit testing, and respiratory protection training
Based on safe sharps work practices, which of the following is not a recommendation?
Needle boxes or sharps containers can be filled to capacity or overfilled before disposal
Which answer is correct regarding sharps containers?
Needle boxes or sharps containers must be located in the immediate vicinity of use and never be allowed to overfill.
Which statement is a good work practice regarding needles and syringes?
Needles and syringes should never be recapped by hand and must be discarded directly into a sharps container or needle box located in the immediate work area.
Who is responsible for conducting the initial written risk assessment for experiments involving biohazards?
Principal Investigator
From the information provided in this module, when should emergency response training be provided?
Prior to starting work and at periodic refresher trainings
Which of the following is the best response applicable to personnel considerations as part of the risk assessment process?
Prior work experience with related biohazards Comfort with the proposed biohazard work The health of the researcher Proficiency with proposed procedures
A clean bench provides what type of protection?
Product protection
Which of the following statements regarding laboratory-acquired infections is not true?
Researchers handling human pathogens have never been infected with an agent as a result of an exposure in the laboratory
Which of the following statements is accurate?
Reusable sharps must be placed in a labeled, rigid container containing disinfectant or in a rigid container for reprocessing to protect handlers until they are decontaminated.
Salmonella and shigella are examples of which Risk Group agents?
Risk Group 2
In Risk Group Comparison Table, Gwladys Caspars' quick guide statement of "don't touch it refers to biohazards in which Risk Group?
Risk Group 2 (Gwladys Caspar uses "don't touch it" to reference the likely ways a Risk Group 2 pathogen can enter the body; through breaks in skin, or through non-intact skin and by contact of facial mucous membranes by contaminated hands (or via direct splashing to these areas).)
Back-fastening gowns are generally associated with which Risk Group?
Risk Group 3
Mycobacterium tuberculosis, a human pathogen with an inhalation route of exposure, a low infectious dose and is capable of causing severe disease, including death, is representative of which Risk Group?
Risk Group 3 (Mycobacterium tuberculosis is a Risk Group 3 pathogen, that can be transmitted via the airborne route, and has an infectious dose of 10 and can kill up to 50% of those who do not receive treatment.)
Based on the information in this module, respirators are generally recommended for researchers handling biohazards classified at which Risk Group?
Risk Group 3 or 4
Ebola virus is an example of a pathogen associated with which Risk Group?
Risk group 4 Ebola virus is a Risk Group 4 pathogen.
The following elements are associated with the pathogen in performing a risk assessment:
Routes of exposure Virulence Communicability Infectious dose
Which of the following is the best location for the placement of a sharps container?
Sharps containers shall be placed in the immediate vicinity of use to permit prompt disposal of a used sharp
What is the best reason below why snap cap tubes should not be used for the centrifugation of biohazards?
Snap cap tubes are not perfectly sealed and the forces during centrifugation may force leakage around the top of the tube and cap
Which statement best reflects the introduction of a new safe sharps device into use?
Staff must have a chance to practice using the device under safe non-hazardous conditions
Which of the factors is more related to biohazard risk assessment than the others?
The Pathogen and its risk classification (The pathogen, the proposed procedures and the personnel who will be performing the work are three core elements of risk assessment for biohazards.)
Which of the following best describes the testing and certification of biosafety cabinets?
The biosafety cabinet should be tested and certified prior to use and periodically thereafter (at least annually).
Which of the following is not a component of the medical surveillance program?
The use of a biological safety cabinet to ensure sterility of research materials
In a study reported in this module on unsafe work practices, researchers were observed doing this 2.6 times per observation on average while handling human pathogens.
Touching their eyes, nose, or mouth
If a needle had to be removed from a syringe, what would be the safest way to do so based on safe sharps work practices?
Use a needle box equipped with a needle removal device that permits the needle to fall directly into the needle box or sharps container when removed
According to the safety information in this module the preferred safe method for collecting contaminated broken glass is to?
Use tongs, forceps or other engineering controls to collect them
Which of the following elements must be a component of the BSL3 laboratory design?
Visual airflow indicator at the lab entry
What is the first step in the immediate aftermath of an exposure to a biohazard involving a percutaneous injury (through the skin)?
Wash the exposed area immediately with soap and water for 15 minutes
Which of the following would be considered good practice for cleaning up large pieces of contaminated glass in a laboratory biohazard spill?
Wear gloves, a lab coat, and face protection; use a dust pan and tongs
Which of the following would not be considered a best practice related to the use of PPE based on the information provided in this module?
Wear personal eyeglasses to protect facial mucous membranes when handling hazardous liquids on the open bench in the laboratory.
According to the emergency response module, an emergency water eye wash station should be located in the following location when biohazards have the potential to cause splash or splatter?
Within 10 seconds of the location of potential exposure.
According to this module, a high risk biohazard exposures including those that involve bloodborne pathogens should be evaluated by a healthcare professional in which time frame?
Within 2 hours or as soon as feasible after the exposure.
Only allowing the most experienced and well-trained staff to perform research with biohazards is an example of what type of control?
administrative
Which of the following is not acceptable in a laboratory?
carpeting
As there are no published listings of risk groups for human pathogens anywhere in the world, Principal Investigators must start by developing a Risk Group ranking for the pathogen they propose using in research.
false
Glove boxes that completely isolate biohazards inside a sealed box are ideal for all experiments and should be used by all laboratories working with biohazards.
false
How many core biosafety work practices (or do's and don'ts) are there, according to the National Research Council's book entitled, Prudent Practices for the Handling and Disposal of Infectious Materials?
seven
Laboratories handling biohazards should maintain a biological spill kit consisting of engineering controls to handle and safely dispose of sharps in the laboratory.
true
Potential risk to the community or those outside the laboratory is part of the formal risk assessment.
true
When considering risk from biohazard experiments involving research animals, shedding of the biohazard is an important factor.
true
