Exercise 2 Lab

Which of the following lens can be safely used with immersion oil? a) 100X b) 40X c) 10X d) 100X and 40X e) all of the objective lens

100X

The ocular lenses of our microscopes in lab provide 10X magnification. If you are viewing cells with the 40X objectives lens what would the total magnification be? a) 4X b) 30X c) 40X d) 50X e) 400X

400X

What must be done after using a microscope? a) Have your instructor check the microscope b) Clean all of the lenses with both lens cleaner and lens paper c) Make sure the lowest objective is locked in place d) Move the stage to the top position e) All of these

All of these

The ability to see two objects as different and separate is referred to as ________. a) Parfocality b) Refractive index c) Resolution d) Magnification e) Contrast

Resolution

Which microscope has two oculars and can magnify larger molecules 4X to 50X? a) Stereomicroscope b) Compound light microscope c) Electron microscope d) None of the above

Stereomicroscope

When using a compound light microscope what happens to the field of vision when the magnification increases? a) The field of vision is not affected by increasing the magnification. b) The field of vision deceases when the magnification increases. c) The field of vision increases when the magnification increases.

The field of vision deceases when the magnification increases.

Why should you always use immersion oil when using the 100x objective? a) To protect the 100x lens from scratches. b) To prevent contamination of your sample. c) To allow the 100x lens to better glide over the microscope slide. d) To adjust the light intensity making it more comfortable to view. e) To reduce light refraction allowing better resolution.

To reduce light refraction allowing better resolution.

When would it be advantageous to use a stereomicroscope instead of a compound light microscope? a) When you want to observe larger three-dimensional objects. b) When you want to observe individual bacterial cells. c) When you need to use the highest resolution possible to observe fine details. d) When your sample is translucent and mounted on a slide.

When you want to observe larger three-dimensional objects.

Explain the benefits and limitations of performing a wet mount. (select 1 benefit and 1 limitation) a) allows for study of motility b) allows for detailed cellular visualization c) short term d) kills cells e) good contrast

a) allows for study of motility c) short term

How can you tell whether an organism is a prokaryote or a eukaryote when viewing it using a compound microscope? a) size b) visualization of the nucleus c)visualization of the ribosome type d) shape

size

Select all that apply. Describe two ways you can adjust the light intensity on your compound microscope. a) use the rheostat b) use the iris diaphragm c) use the focusing knob d) use the condensor

use the rheostat use the iris diaphragm