Gel Electrophoresis Assessment #2
Why do gel electrophoresis?
1. To determine which organisms are most closely related. 2. To determine paternity of a child. 3. For crime scene analysis.
distance education/learning
A teaching method in which the teacher and student are not in the same location.
Gel electrophoresis
A technique used to separate segments of DNA based on their size using an electric current
Third step in gel electrophoresis
An electric charge is applied to the gel. The negatively charged DNA moves toward the positive side of the gel.
Results of gel electrophoresis
DNA fragments are separated by size. Smaller fragments move the furthest while larger fragments will be closer to the loading well. These are seen as bands within the gel.
First step in gel electrophoresis
DNA is first cut using special enzymes called restriction enzymes.
Who is the baby's dad?
Dad 2
What is gel electrophoresis for?
It is used to separate DNA fragments based upon size.
Shorter/Smaller DNA fragments
Move faster through the gel and end up further from the wells .
Larger/Longer DNA fragments
Move slower through the gel and end up closer to the wells.
Second step in gel electrophoresis
The DNA sample is loaded into an agarose gel.
Wells
The spaces that are loaded with DNA samples
DNA fingerprint
Unique sequence of DNA base pairs that can be used to identify a person at the molecular level
advantage of distance learning
convenience/flexibility
Bands
lines of DNA located at a certain position in the gel (DNA ladder).