Histotechnician Practice Questions

What is the ideal preparation or concentration of formaldehyde?

10% solution

All of the following choices below are fixatives of choice for electron microscopy, except: a Osmium Tetroxide b Glutaraldehyde c 100% Menthol d Paraformaldehyde

100% Menthol

What is the usual range of temperature of a water bath that removes the paraffin wax from the tissue?

45-50 degrees Celsius

Why is glutaraldehyde the preferred fixative for specimens in electron microscopy? a Glutaraldehyde is much easier to obtain than formaldehyde b Tissues fixed with glutaraldehyde stain well with dyes c Glutaraldehyde does not change the solubility of the fixed tissue d It excels at preserving the detailed structures of the cells and tissues.

It excels at preserving the detailed structures of the cells and tissues.

Which of the following statements is true about head fixation? a It does not preserve overall morphology, but it preserves internal structures. b It preserves overall morphology, but it does preserve internal structures. c It preserves overall morphology and preserves the internal structures. d It does not preserve both overall morphology and the internal structures.

It preserves overall morphology, but it does preserve internal structures.

The following are the preferred fixatives to be used on lipids except: a Frozen section b Osmium tetroxide c Glutaraldehyde d Neutral buffered formalin

Neutral buffered formalin

Calcium and other calcified material may be removed from the tissue by any of the following agents, except: a Cheleating agent b Oils c Acids d Electrophoresis

Oils

The type of fixative is preferred for the diagnosis of early degenerative processes and tissue necrosis. a Muller's Fluid b Heidenhain's Susa c Zenker-formal d Orth's Fluid

Orth's Fluid

Which of the following is not an example of a nuclear fixative? a Flemming's fluid b Orth's fluid c Newcomer's fluid d Heidenhain's Susa

Orth's fluid

It is used in fixing brain tissue for the diagnosis of rabies.

Acetone

A histotechnician receives a sample of bone marrow for biopsy. What fixative of choice is suitable for bone marrow preservation?

B-5 Fixative

This type of knife measures usually about 120 mm in length with both sides concave and it is recommended for cutting paraffin embedded section on a rotary microtome.

Biconcave knife

Which of the following tissue samples is fixed first before sectioning or grossing?

Brain

Which of the following choices is an excellent dehydrating agent? a Ethyl alcohol b Dioxane c Triethyl Phsphate d Acetone

Dioxane

Don recalls that the following items are also needed furing microtomy, aside from: a Clean slides b Permanent marker c Water bath d Drying oven

Permanent marker

What do you call the process whereby tissues are cut into uniformly thin slices or section with the aid of a machine?

Sectioning

This microtome was developed by Adams in 1789.

Sliding microtome

All of the following statements are true about why lengthwise vertical scratches are seen on the sections, except: a Dirty embedding medium b Nicks on the edge of the knife c The tilt of the knife is too great d The knife is sharp

The knife is sharp

Which of the following best described fixation? a A procedure adopted to kill, harden and preserve the tissue for microscopic study by the use of substance like mercuric chloride. b The procedure in which the ionic materials are removed from the tissue. c A procedure adopted to killed, harden and preserve the tissue for microscopic study by the use of substance like xylene. d The process of removing intracellular and extracellular H2O,

A procedure adopted to kill, harden and preserve the tissue for microscopic study by the use of substance like mercuric chloride.