HOL: Biomolecular Techniques

125 bp

Based on the electrophoresis results in the image, the approximate size of Sample 2 is_____. - 40 pb - 85 pb - 125 bp - 150 pb

Study of the structure, function, and formation of nucleic acids and proteins.

Molecular biology

Biomolecular technique used to create copies of DNA regions outside of an organism.

PCR

_____ is a biomolecular technique utilizing primers, template DNA, and Taq polymerase to produce copies of DNA fragments. - PCR - Bacterial transformation - Gel electrophoresis - ELISA

PCR

Chemical reaction involving the transfer of genetic information to ribosomes where amino acids are assembled into chains.

Protein synthesis

Molecule composed of genetic material from more than one source.

Recombinant DNA

Cold _____ is used to precipitate DNA from -solution during DNA extraction. - alcohol - detergent - agarose - saline

alcohol

_____ is a biomolecular technique for cloning recombinant DNA in living cells. - PCR - Bacterial transformation - Gel electrophoresis - Tissue culturing

bacterial transformation

Gel electrophoresis uses _____ to separate DNA fragments. - solvent - light - sound - current

current

True or false: During gel electrophoresis, the largest DNA fragments move fastest through the agarose gel.

false

True or false: Extracted plant DNA appears as yellow crystals.

false

True or false: Large fragments of DNA travel the greatest distance during gel electrophoresis.

false

True or false: PCR utilizes low temperatures to denature and synthesize DNA.

false

DNA is composed of ____ nucleotides that create the genetic code for all organisms. - three - four - six - eight

four

DNA is composed of two chains of _____. - proteins - lipids - nucleotides - enzymes

nucleotides

Recombinant DNA is formed in the laboratory by cementing DNA fragments into circular _____ using DNA ligase. - bacteria - plasmids - enzymes - clones

plasmids

Enzyme that cements the ends of fragments to form a single molecule.

DNA ligase

True or False: Gel electrophoresis uses electric current to separate molecules based on size and charge.

true

True or false: Gene cutting software provides an array of restriction enzymes and cutting location options for researchers to select from when creating recombinant plasmids.

true

True or false: Molecular biologists manipulate DNA to control protein synthesis.

true

True or false: Restriction enzymes are proteins that recognize gene sequences and cut DNA at specific locations.

true