Lab Safety and Microscope Extra Credit

a, c

Goggles or appropriate safety glasses protect the eyes from (Check all that apply) a. chemical splashes b. fumes of preservatives used on anatomy specimens. c. UV exposure when using UV radiation in the laboratory d. burns from the hot plate or Bunsen burner

b

In which circumstance is food and/or beverage allowed in the laboratory? a. if containers are kept out of sight b. never c. if containers are sealed d. if containers are covered

a. 1 b. 3 c. 6 d. 2 e. 4 f. 5

Indicate the correct order of steps as you bring an object into focus under high power. a. Place slide on stage and secure swith stage clips. b. Focus specimen on low power using coarse focus adjustment. c. Readjust fine focus under high power to produce the sharpest image. d. Turn on the light and center the specimen over the light source. e. Use fine focus to sharpen the image under low power. f. Rotate high power lens into position.

b

Lab coats, gloves, face masks, and goggles are all examples of a. fashion items preferred by biology students b. personal protective equipment c. items supplied by your college or university

1. Concentrates light onto the specimen 2. After light passes through the specimen, it next enters this lens system 3. Platform that supports a microscope slide 4. Increases or decreases the light intensity 5. Causes stage (or objective lens) to move upward or downward

Match the names of the microscope parts in column A with the descriptions in column B. Place the letter of your choice in the space provided. 1. Condenser 2. Objective lens system 3. Stage 4. Iris diaphram 5. Adjustment knob

d

The objective lenses of the compound light microscope are attached to the a. stage. b. base. c. body tube. d. rotating nosepiece.

F

Using a cell phone in lab is permissible since it can be used for timing experiments. (T/F)

a, b, c

What should be done to prepare or deal with a laboratory emergency? a. Know the location of safety equipment such as the fire extinguisher. b. Notify your instructor regarding spills or non-emergency situations. c. Call 911 if an emergency occurs. d. Take antibiotics regularly before attending class in case of spills. e. Conduct all lab procedures under a fume hood.

c

When preparing a wet mount specimen for viewing, it should be covered with _______. a. clear paper b. another glass slide c. a coverslip d. transparent tape

400

When the high power (40x) objective is used the total magnification will be ___x.

100

When the low power (10x) objective is used the total magnification will be ___x.

1000

When the oil immersion (100x) objective is used the total magnification will be ___x.

40

When the scanning (4x) objective is used the total magnification will be ___x.

b, c

Which items can be discarded in a regular trash can? (Check all that apply) a. paper towels used to wipe up bacterial spills b. uncontaminated waste paper c. wrappers from swabs or from disposable pipetttes d. used glass slides

d

Which objective lens provides the highest total magnification? a. scan b. low power c. high power d. oil immersion

a, b, c, d, e

Which of the following are potential hazards which you might encounter in the laboratory? (Check all that apply) a. infectious microorganisms b. open flames c. hot surfaces d. corrosive chemicals e. sharp tools or broken glass f. gamma radiation g. magnetic fields

a

Which of the following components of microscopy will affect the area of a slide seen in the field of view? a. magnification b. contrast c. resolution

a, b, e

Which of the following items can be washed in the sink after use in the laboratory? (Check all that apply) a. dissection tools b. glassware that was not used with hazardous chemicals c. E. coli broth culture tubes d. inoculating loops e. dissection trays

a, b, c, f

Which of the following items should be disposed of in the sharps container? (Check all that apply) a. needles b. glass pipettes c. broken glass d. gloves e. agar plates f. scalpel blades

a, b, c, d

Who and/or what is protected by lab safety protocols? (Check all that apply) a. Students doing lab activities b. Lab instructors and technical assistants. c. Anyone who walks into the lab. d. The physical lab space and the equipment housed within the lab.